Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091306392> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2091306392 endingPage "621" @default.
- W2091306392 startingPage "613" @default.
- W2091306392 abstract "Expression of both bovine adrenodoxin (ADX) and NADPH-adrenodoxin reductase (ADR) were examined in Saccharomyces cerevisiae. Three ADX and two ADR expression plasmids were constructed by inserting each of the corresponding cDNA fragments between the yeast alcohol dehydrogenase I promoter and terminator of the expression vector pAAH5N. Plasmids pAX and pMX contained the coding region for the precursor and mature ADX, respectively, while pCMX carried the mature ADX preceded by the mitochondrial signal of yeast cytochrome c oxidase subunit IV (COX IV). Similarly, pMR and pCMR coded for mature ADR without and with the mitochondrial signal of yeast COX IV, respectively. Transformed S. cerevisiae AH22[[unk]°]/pAX cells produced the ADX precursor, while AH22[[unk]°]/pMX and AH22[[unk]°]/pCMX cells produced mature ADX (mat-ADX) and modified ADX (mat-COX/ADX), respectively. Mat-ADX and mat-COX/ADX were found mainly in the cytosolic and mitochondrial fractions, respectively, and showed cytochrome c reductase activity. AH22[[unk]+]/pMR and AH22[[unk]+]/pCMR cells produced mature ADR (mat-ADR) and modified ADR (mat-COX/ADR), respectively. Mat-ADR lacking the mitochondrial signal was found in the cytosolic fraction and exhibited cytochrome c reductase activity, while mat-COX/ADR was localized in the mitochondrial fraction, but showed no reductase activity. In an in vitro reconstituted system consisting of both mat-COX/ADX- and mat-ADR-containing fractions, bovine P450scc converted cholesterol into pregnenolone. Thus mat-COX/ADX and mat-ADR produced in the yeast can transfer electrons from NADPH to P450scc." @default.
- W2091306392 created "2016-06-24" @default.
- W2091306392 creator A5006942403 @default.
- W2091306392 creator A5019561023 @default.
- W2091306392 creator A5036846494 @default.
- W2091306392 creator A5056859828 @default.
- W2091306392 creator A5062265452 @default.
- W2091306392 date "1991-10-01" @default.
- W2091306392 modified "2023-10-17" @default.
- W2091306392 title "Expression of Bovine Adrenodoxin and NADPH-Adrenodoxin Reductase cDNAs in<i>Saccharomyces cerevisiae</i>" @default.
- W2091306392 cites W1507851645 @default.
- W2091306392 cites W1602081712 @default.
- W2091306392 cites W1674435604 @default.
- W2091306392 cites W1682948375 @default.
- W2091306392 cites W1776107594 @default.
- W2091306392 cites W1825170318 @default.
- W2091306392 cites W1966183056 @default.
- W2091306392 cites W1972737942 @default.
- W2091306392 cites W1980829113 @default.
- W2091306392 cites W1982183234 @default.
- W2091306392 cites W1994803993 @default.
- W2091306392 cites W2003065309 @default.
- W2091306392 cites W2019043837 @default.
- W2091306392 cites W2023871900 @default.
- W2091306392 cites W2036216281 @default.
- W2091306392 cites W2038556205 @default.
- W2091306392 cites W2043413810 @default.
- W2091306392 cites W2046559874 @default.
- W2091306392 cites W2048952309 @default.
- W2091306392 cites W2052690462 @default.
- W2091306392 cites W2062680845 @default.
- W2091306392 cites W2087742245 @default.
- W2091306392 cites W2260884575 @default.
- W2091306392 cites W97101753 @default.
- W2091306392 doi "https://doi.org/10.1089/dna.1991.10.613" @default.
- W2091306392 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1930696" @default.
- W2091306392 hasPublicationYear "1991" @default.
- W2091306392 type Work @default.
- W2091306392 sameAs 2091306392 @default.
- W2091306392 citedByCount "28" @default.
- W2091306392 countsByYear W20913063922012 @default.
- W2091306392 countsByYear W20913063922017 @default.
- W2091306392 countsByYear W20913063922021 @default.
- W2091306392 countsByYear W20913063922023 @default.
- W2091306392 crossrefType "journal-article" @default.
- W2091306392 hasAuthorship W2091306392A5006942403 @default.
- W2091306392 hasAuthorship W2091306392A5019561023 @default.
- W2091306392 hasAuthorship W2091306392A5036846494 @default.
- W2091306392 hasAuthorship W2091306392A5056859828 @default.
- W2091306392 hasAuthorship W2091306392A5062265452 @default.
- W2091306392 hasConcept C123249941 @default.
- W2091306392 hasConcept C134651460 @default.
- W2091306392 hasConcept C153911025 @default.
- W2091306392 hasConcept C181199279 @default.
- W2091306392 hasConcept C2777576037 @default.
- W2091306392 hasConcept C2779222958 @default.
- W2091306392 hasConcept C2780768313 @default.
- W2091306392 hasConcept C2910521418 @default.
- W2091306392 hasConcept C526171541 @default.
- W2091306392 hasConcept C55493867 @default.
- W2091306392 hasConcept C80446465 @default.
- W2091306392 hasConcept C86803240 @default.
- W2091306392 hasConcept C98539663 @default.
- W2091306392 hasConceptScore W2091306392C123249941 @default.
- W2091306392 hasConceptScore W2091306392C134651460 @default.
- W2091306392 hasConceptScore W2091306392C153911025 @default.
- W2091306392 hasConceptScore W2091306392C181199279 @default.
- W2091306392 hasConceptScore W2091306392C2777576037 @default.
- W2091306392 hasConceptScore W2091306392C2779222958 @default.
- W2091306392 hasConceptScore W2091306392C2780768313 @default.
- W2091306392 hasConceptScore W2091306392C2910521418 @default.
- W2091306392 hasConceptScore W2091306392C526171541 @default.
- W2091306392 hasConceptScore W2091306392C55493867 @default.
- W2091306392 hasConceptScore W2091306392C80446465 @default.
- W2091306392 hasConceptScore W2091306392C86803240 @default.
- W2091306392 hasConceptScore W2091306392C98539663 @default.
- W2091306392 hasIssue "8" @default.
- W2091306392 hasLocation W20913063921 @default.
- W2091306392 hasLocation W20913063922 @default.
- W2091306392 hasOpenAccess W2091306392 @default.
- W2091306392 hasPrimaryLocation W20913063921 @default.
- W2091306392 hasRelatedWork W1966810776 @default.
- W2091306392 hasRelatedWork W1971276266 @default.
- W2091306392 hasRelatedWork W1982183234 @default.
- W2091306392 hasRelatedWork W1990538424 @default.
- W2091306392 hasRelatedWork W2012963288 @default.
- W2091306392 hasRelatedWork W2018991487 @default.
- W2091306392 hasRelatedWork W2024440231 @default.
- W2091306392 hasRelatedWork W2057182257 @default.
- W2091306392 hasRelatedWork W2092708805 @default.
- W2091306392 hasRelatedWork W2122507575 @default.
- W2091306392 hasVolume "10" @default.
- W2091306392 isParatext "false" @default.
- W2091306392 isRetracted "false" @default.
- W2091306392 magId "2091306392" @default.
- W2091306392 workType "article" @default.