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- W2091351608 abstract "Murine fibrosarcoma L929 cells have been used to test efficacy of proinflammatory cytokine TNFα. In the present study, we reported on protective effect of type I collagen gel used as L929 cell culture. L929 cell grew and proliferated well on collagen gel. However, the L929 cells exhibited cobblestone-like morphology which was much different from the spread fusiform shape when cultured on conventional cell dishes as well as the cells tended to aggregate. On conventional cell culture dishes, the cells treated with TNFα became round in shape and eventually died in a necroptotic manner. The cells cultured on collagen gel, however, were completely unaffected. TNFα treatment was reported to induce autophagy in L929 cells on the plastic dish, and therefore we investigated the effect of collagen gel on induction of autophagy. The results indicated that autophagy induced by TNFα treatment was much reduced when the cells were cultured on collagen gel. In conclusion, type I collagen gel protected L929 cell from TNFα-induced cell death." @default.
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- W2091351608 date "2015-02-01" @default.
- W2091351608 modified "2023-09-28" @default.
- W2091351608 title "Type I collagen gel protects murine fibrosarcoma L929 cells from TNFα-induced cell death" @default.
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- W2091351608 doi "https://doi.org/10.1016/j.bbrc.2015.01.051" @default.
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