FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091369148> ?p ?o ?g. }

• W2091369148 endingPage "29" @default.
• W2091369148 startingPage "17" @default.
• W2091369148 abstract "The RuvC protein of Escherichia coli resolves Holliday intermediates in recombination and DNA repair by a dual strand incision mechanism targeted to specific DNA sequences located symmetrically at the crossover. Two classes of amino acid substitutions are described that provide new insights into the sequence-specificity of the resolution reaction. The first includes D7N and G14S, which modify or eliminate metal binding and prevent catalysis. The second, defined by G114D, G114N, and A116T, interfere with the ability of RuvC to cleave at preferred sequences, but allow resolution at non-consensus target sites. All five mutant proteins bind junction DNA and impose an open conformation. D7N and G14S fail to induce hypersensitivity to hydroxyl radicals, a property of RuvC previously thought to reflect junction opening. A different mechanism is proposed whereby ferrous ions are co-ordinated in the complex to induce a high local concentration of radicals. The open structure imposed by wild-type RuvC in Mg2+ is similar to that observed previously using a junction with a different stacking preference. G114D and A116T impose slightly altered structures. This subtle change may be sufficient to explain the failure of these proteins to cleave the sequences normally preferred. Gly114 and Ala116 residues link two α-helices lining the wall of the catalytic cleft in each subunit of RuvC. We suggest that substitutions at these positions realign these helices and interfere with the ability to establish base-specific contacts at resolution hotspots." @default.
• W2091369148 created "2016-06-24" @default.
• W2091369148 creator A5007530037 @default.
• W2091369148 creator A5024799692 @default.
• W2091369148 creator A5025661928 @default.
• W2091369148 creator A5038898027 @default.
• W2091369148 creator A5042270506 @default.
• W2091369148 creator A5054117046 @default.
• W2091369148 creator A5073689309 @default.
• W2091369148 date "1998-08-01" @default.
• W2091369148 modified "2023-10-02" @default.
• W2091369148 title "Sequence-specificity of holliday junction resolution: identification of RuvC mutants defective in metal binding and target site recognition" @default.
• W2091369148 cites W1521689319 @default.
• W2091369148 cites W154887227 @default.
• W2091369148 cites W1553481351 @default.
• W2091369148 cites W1571933804 @default.
• W2091369148 cites W1595811992 @default.
• W2091369148 cites W1714509470 @default.
• W2091369148 cites W1819552352 @default.
• W2091369148 cites W1873199501 @default.
• W2091369148 cites W1923696985 @default.
• W2091369148 cites W1967071624 @default.
• W2091369148 cites W1972852754 @default.
• W2091369148 cites W1975329612 @default.
• W2091369148 cites W1982418584 @default.
• W2091369148 cites W1985052477 @default.
• W2091369148 cites W1986398477 @default.
• W2091369148 cites W1986799468 @default.
• W2091369148 cites W1989787656 @default.
• W2091369148 cites W1991261520 @default.
• W2091369148 cites W1999659932 @default.
• W2091369148 cites W1999746794 @default.
• W2091369148 cites W1999775190 @default.
• W2091369148 cites W2001167026 @default.
• W2091369148 cites W2002003212 @default.
• W2091369148 cites W2014570065 @default.
• W2091369148 cites W2015744531 @default.
• W2091369148 cites W2020558769 @default.
• W2091369148 cites W2028622989 @default.
• W2091369148 cites W2028844154 @default.
• W2091369148 cites W2029322879 @default.
• W2091369148 cites W2029887349 @default.
• W2091369148 cites W2045968012 @default.
• W2091369148 cites W2054341159 @default.
• W2091369148 cites W2057201443 @default.
• W2091369148 cites W2066544743 @default.
• W2091369148 cites W2074033471 @default.
• W2091369148 cites W2092073545 @default.
• W2091369148 cites W2094929824 @default.
• W2091369148 cites W2138222836 @default.
• W2091369148 cites W2147220402 @default.
• W2091369148 cites W2160393299 @default.
• W2091369148 cites W2170075515 @default.
• W2091369148 cites W2619406833 @default.
• W2091369148 doi "https://doi.org/10.1006/jmbi.1998.1934" @default.
• W2091369148 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9680472" @default.
• W2091369148 hasPublicationYear "1998" @default.
• W2091369148 type Work @default.
• W2091369148 sameAs 2091369148 @default.
• W2091369148 citedByCount "13" @default.
• W2091369148 countsByYear W20913691482012 @default.
• W2091369148 countsByYear W20913691482013 @default.
• W2091369148 countsByYear W20913691482017 @default.
• W2091369148 crossrefType "journal-article" @default.
• W2091369148 hasAuthorship W2091369148A5007530037 @default.
• W2091369148 hasAuthorship W2091369148A5024799692 @default.
• W2091369148 hasAuthorship W2091369148A5025661928 @default.
• W2091369148 hasAuthorship W2091369148A5038898027 @default.
• W2091369148 hasAuthorship W2091369148A5042270506 @default.
• W2091369148 hasAuthorship W2091369148A5054117046 @default.
• W2091369148 hasAuthorship W2091369148A5073689309 @default.
• W2091369148 hasConcept C102744134 @default.
• W2091369148 hasConcept C104317684 @default.
• W2091369148 hasConcept C12554922 @default.
• W2091369148 hasConcept C130965112 @default.
• W2091369148 hasConcept C143065580 @default.
• W2091369148 hasConcept C185581394 @default.
• W2091369148 hasConcept C185592680 @default.
• W2091369148 hasConcept C19014433 @default.
• W2091369148 hasConcept C552990157 @default.
• W2091369148 hasConcept C55493867 @default.
• W2091369148 hasConcept C71240020 @default.
• W2091369148 hasConcept C86803240 @default.
• W2091369148 hasConceptScore W2091369148C102744134 @default.
• W2091369148 hasConceptScore W2091369148C104317684 @default.
• W2091369148 hasConceptScore W2091369148C12554922 @default.
• W2091369148 hasConceptScore W2091369148C130965112 @default.
• W2091369148 hasConceptScore W2091369148C143065580 @default.
• W2091369148 hasConceptScore W2091369148C185581394 @default.
• W2091369148 hasConceptScore W2091369148C185592680 @default.
• W2091369148 hasConceptScore W2091369148C19014433 @default.
• W2091369148 hasConceptScore W2091369148C552990157 @default.
• W2091369148 hasConceptScore W2091369148C55493867 @default.
• W2091369148 hasConceptScore W2091369148C71240020 @default.
• W2091369148 hasConceptScore W2091369148C86803240 @default.
• W2091369148 hasIssue "1" @default.
• W2091369148 hasLocation W20913691481 @default.
• W2091369148 hasLocation W20913691482 @default.

• next