Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091392394> ?p ?o ?g. }
- W2091392394 endingPage "3612" @default.
- W2091392394 startingPage "3596" @default.
- W2091392394 abstract "The sesquiterpene alantolactone counteracts malignancy, an effect at least in part due to stimulation of suicidal death or apoptosis of tumor cells. Signaling of alantolactone induced apoptosis involves altered gene expression and mitochondrial depolarization. Erythrocytes lack mitochondria and nuclei but may enter suicidal death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surface. Cellular mechanisms involved in triggering of eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i) and oxidative stress. The present study explored, whether alantolactone stimulates eryptosis. To this end, erythrocyte volume was estimated from forward scatter, phosphatidylserine-exposure at the erythrocyte surface from FITC-annexin-V-binding, [Ca2+]i from Fluo3-fluorescence, ceramide abundance from binding of fluorescent antibodies, and oxidative stress from 2',7'-dichlorodihydrofluorescein-diacetate (DCFDA) fluorescence. As a result, a 48 h exposure of human erythrocytes to alantolactone (≥20 μM) significantly decreased erythrocyte forward scatter and increased the percentage of annexin-V-binding cells. Alantolactone significantly increased Fluo3 fluorescence (60 μM), ceramide abundance (60 μM) and DCFDA fluorescence (≥40 μM). The effect of alantolactone (60 μM) on annexin-V-binding was not significantly modified by removal of extracellular Ca2+. In conclusion, alantolactone stimulates suicidal erythrocyte death or eryptosis, an effect paralleled by increase of [Ca2+]i, ceramide abundance and oxidative stress." @default.
- W2091392394 created "2016-06-24" @default.
- W2091392394 creator A5014570972 @default.
- W2091392394 creator A5022548921 @default.
- W2091392394 creator A5050267245 @default.
- W2091392394 creator A5059633094 @default.
- W2091392394 creator A5084208566 @default.
- W2091392394 date "2014-12-22" @default.
- W2091392394 modified "2023-10-16" @default.
- W2091392394 title "Triggering of Programmed Erythrocyte Death by Alantolactone" @default.
- W2091392394 cites W13114315 @default.
- W2091392394 cites W1578593243 @default.
- W2091392394 cites W1964268088 @default.
- W2091392394 cites W1964416229 @default.
- W2091392394 cites W1971967354 @default.
- W2091392394 cites W1972865309 @default.
- W2091392394 cites W1977397908 @default.
- W2091392394 cites W1979055687 @default.
- W2091392394 cites W1979397159 @default.
- W2091392394 cites W1980548122 @default.
- W2091392394 cites W1982175258 @default.
- W2091392394 cites W1982745075 @default.
- W2091392394 cites W1983943582 @default.
- W2091392394 cites W1984372445 @default.
- W2091392394 cites W1984504487 @default.
- W2091392394 cites W1984765893 @default.
- W2091392394 cites W1984851725 @default.
- W2091392394 cites W1987065544 @default.
- W2091392394 cites W1988212011 @default.
- W2091392394 cites W1989482850 @default.
- W2091392394 cites W1990356838 @default.
- W2091392394 cites W1991516089 @default.
- W2091392394 cites W1996765306 @default.
- W2091392394 cites W1997035621 @default.
- W2091392394 cites W1999196238 @default.
- W2091392394 cites W2002159717 @default.
- W2091392394 cites W2003505368 @default.
- W2091392394 cites W2009868844 @default.
- W2091392394 cites W2013300597 @default.
- W2091392394 cites W2014604485 @default.
- W2091392394 cites W2015426350 @default.
- W2091392394 cites W2015817052 @default.
- W2091392394 cites W2017672080 @default.
- W2091392394 cites W2019469219 @default.
- W2091392394 cites W2021722692 @default.
- W2091392394 cites W2022307600 @default.
- W2091392394 cites W2022371308 @default.
- W2091392394 cites W2024583929 @default.
- W2091392394 cites W2026962502 @default.
- W2091392394 cites W2029665894 @default.
- W2091392394 cites W2031903488 @default.
- W2091392394 cites W2036373385 @default.
- W2091392394 cites W2036961219 @default.
- W2091392394 cites W2039430846 @default.
- W2091392394 cites W2039616271 @default.
- W2091392394 cites W2040515425 @default.
- W2091392394 cites W2043350204 @default.
- W2091392394 cites W2046126455 @default.
- W2091392394 cites W2046588089 @default.
- W2091392394 cites W2046719422 @default.
- W2091392394 cites W2048339738 @default.
- W2091392394 cites W2050609638 @default.
- W2091392394 cites W2052123857 @default.
- W2091392394 cites W2056501114 @default.
- W2091392394 cites W2059716230 @default.
- W2091392394 cites W2059888207 @default.
- W2091392394 cites W2060106509 @default.
- W2091392394 cites W2065196084 @default.
- W2091392394 cites W2068631067 @default.
- W2091392394 cites W2070841180 @default.
- W2091392394 cites W2072409837 @default.
- W2091392394 cites W2074728848 @default.
- W2091392394 cites W2076186362 @default.
- W2091392394 cites W2078779135 @default.
- W2091392394 cites W2079389145 @default.
- W2091392394 cites W2080046576 @default.
- W2091392394 cites W2083075385 @default.
- W2091392394 cites W2084883771 @default.
- W2091392394 cites W2088506278 @default.
- W2091392394 cites W2089281127 @default.
- W2091392394 cites W2090421762 @default.
- W2091392394 cites W2092267921 @default.
- W2091392394 cites W2093149218 @default.
- W2091392394 cites W2094743161 @default.
- W2091392394 cites W2097098920 @default.
- W2091392394 cites W2098000156 @default.
- W2091392394 cites W2114724623 @default.
- W2091392394 cites W2119337157 @default.
- W2091392394 cites W2123356000 @default.
- W2091392394 cites W2127883583 @default.
- W2091392394 cites W2129714969 @default.
- W2091392394 cites W2132966139 @default.
- W2091392394 cites W2136690044 @default.
- W2091392394 cites W2138834196 @default.
- W2091392394 cites W2143381934 @default.
- W2091392394 cites W2144099275 @default.
- W2091392394 cites W2146056429 @default.
- W2091392394 cites W2165294395 @default.