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- W2091424059 abstract "Microbially influenced corrosion (MIC) is being increasingly recognised as a serious problem. To investigate the role of MIC, radiotracer activity and lipid biomass measurements were performed on samples from offshore and on‐shore natural gas transmission systems. These measurements evaluated the biomass and metabolism of microbial communities residing inside transmission pipelines. Aqueous and nonaqueous hydrocarbon samples from liquid separators, sludge catchers and nodules attached to pipe walls were aseptically recovered and inoculated into anaerobic tubes for radiotracer time course experiments or preserved with chloroform‐methanol for total lipid analyses. MPN enrichments and phospholipid biomass determinations estimated microbial populations of 104—107 cells per gram in several samples. General microbial metabolism was demonstrated by [l‐14C]acetate incorporation into lipids and by [14C]CO2 production from [U‐14C]glucose. [14C]Acetate was slowly mineralised to 14CO2 without significant methane production. [14C]Acetate was produced by fermentation of [14C]glucose, [14C]palmitate and by hydrogen mediated acetogenesis in the presence of [I4C]CO2. In one location acetogenesis from hydrogen and carbon dioxide accounted for 0–7 mmol.l‐1 of acetate production per week. These results demonstrated that microorganisms could utilise natural gas impurities to produce organic acids. This activity could adversely affect the structural integrity (MIC) of high pressure natural gas pipelines." @default.
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- W2091424059 date "1991-07-01" @default.
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- W2091424059 title "Anaerobic microbial activities including hydrogen mediated acetogenesis within natural gas transmission lines" @default.
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- W2091424059 doi "https://doi.org/10.1080/08927019109378181" @default.
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