FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091466621> ?p ?o ?g. }

• W2091466621 endingPage "580" @default.
• W2091466621 startingPage "567" @default.
• W2091466621 abstract "This is the first in a series of three papers [see also Mulquiney and Kuchel (1999) Biochem. J. 342, 579-594; Mulquiney and Kuchel (1999) Biochem. J. 342, 595-602] that present a detailed mathematical model of erythrocyte metabolism which explains the regulation and control of 2,3-bisphosphoglycerate (2,3-BPG) metabolism. 2,3-BPG is a modulator of haemoglobin oxygen affinity and hence plays an important role in blood oxygen transport and delivery. This paper presents an in vivo kinetic characterization of 2,3-BPG synthase/phosphatase (BPGS/P), the enzyme that catalyses both the synthesis and degradation of 2,3-BPG. Much previous work had indicated that the behaviour of this enzyme in vitro is markedly different from that in vivo. 13C and 31P NMR were used to monitor the time courses of selected metabolites when erythrocytes were incubated with or without [U-13C]glucose. Simulations of the experimental time courses were then made. By iteratively changing the parameters of the BPGS/P part of the model until a good match between the NMR-derived data and simulations were achieved, it was possible to characterize BPGS/P kineticallyin vivo. This work revealed that: (1) the pH-dependence of the synthase activity results largely from a strong co-operative inhibition of the synthase activity by protons; (2) 3-phosphoglycerate and 2-phosphoglycerate are much weaker inhibitors of 2,3-BPG phosphatase in vivo than in vitro; (3) the Km of BPGS/P for 2,3-BPG is significantly higher than that measured in vitro; (4) the maximal activity of the phosphatase in vivo is approximately twice that in vitro, when Pi is the sole activator (second substrate); and (5) 2-phosphoglycollate appears to play no role in the activation of the phosphatase in vivo. Using the newly determined kinetic parameters, the percentage of glycolytic carbon flux that passes through the 2,3-BPG shunt in the normal in vivo steady state was estimated to be 19%." @default.
• W2091466621 created "2016-06-24" @default.
• W2091466621 creator A5002951013 @default.
• W2091466621 creator A5065404276 @default.
• W2091466621 creator A5065920375 @default.
• W2091466621 date "1999-09-05" @default.
• W2091466621 modified "2023-10-16" @default.
• W2091466621 title "Model of 2,3-bisphosphoglycerate metabolism in the human erythrocyte based on detailed enzyme kinetic equations1: in vivo kinetic characterization of 2,3-bisphosphoglycerate synthase/phosphatase using 13C and 31P NMR" @default.
• W2091466621 cites W1446846642 @default.
• W2091466621 cites W1495151785 @default.
• W2091466621 cites W1499750176 @default.
• W2091466621 cites W1543292456 @default.
• W2091466621 cites W1553901576 @default.
• W2091466621 cites W1559714449 @default.
• W2091466621 cites W1562626340 @default.
• W2091466621 cites W1567378741 @default.
• W2091466621 cites W1577040608 @default.
• W2091466621 cites W1599053831 @default.
• W2091466621 cites W1603996635 @default.
• W2091466621 cites W1607315648 @default.
• W2091466621 cites W1643490512 @default.
• W2091466621 cites W1651185880 @default.
• W2091466621 cites W172232536 @default.
• W2091466621 cites W181283077 @default.
• W2091466621 cites W1915957948 @default.
• W2091466621 cites W1964933261 @default.
• W2091466621 cites W1967579050 @default.
• W2091466621 cites W1972887240 @default.
• W2091466621 cites W1974399076 @default.
• W2091466621 cites W1983950473 @default.
• W2091466621 cites W1991559584 @default.
• W2091466621 cites W1998567323 @default.
• W2091466621 cites W1998598808 @default.
• W2091466621 cites W1998903281 @default.
• W2091466621 cites W2013744832 @default.
• W2091466621 cites W2017329508 @default.
• W2091466621 cites W2024802513 @default.
• W2091466621 cites W2032378325 @default.
• W2091466621 cites W2052782582 @default.
• W2091466621 cites W2053648350 @default.
• W2091466621 cites W2067180894 @default.
• W2091466621 cites W2068702774 @default.
• W2091466621 cites W2086558224 @default.
• W2091466621 cites W2087155387 @default.
• W2091466621 cites W2089325952 @default.
• W2091466621 cites W2094751995 @default.
• W2091466621 cites W2106742434 @default.
• W2091466621 cites W2109189469 @default.
• W2091466621 cites W2110470498 @default.
• W2091466621 cites W2171749735 @default.
• W2091466621 cites W2272998872 @default.
• W2091466621 cites W2395966321 @default.
• W2091466621 cites W2396050323 @default.
• W2091466621 cites W2411801038 @default.
• W2091466621 cites W2426069131 @default.
• W2091466621 cites W4289974761 @default.
• W2091466621 cites W47016903 @default.
• W2091466621 cites W86248895 @default.
• W2091466621 doi "https://doi.org/10.1042/bj3420567" @default.
• W2091466621 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1220498" @default.
• W2091466621 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10477268" @default.
• W2091466621 hasPublicationYear "1999" @default.
• W2091466621 type Work @default.
• W2091466621 sameAs 2091466621 @default.
• W2091466621 citedByCount "68" @default.
• W2091466621 countsByYear W20914666212012 @default.
• W2091466621 countsByYear W20914666212013 @default.
• W2091466621 countsByYear W20914666212014 @default.
• W2091466621 countsByYear W20914666212015 @default.
• W2091466621 countsByYear W20914666212016 @default.
• W2091466621 countsByYear W20914666212017 @default.
• W2091466621 countsByYear W20914666212018 @default.
• W2091466621 countsByYear W20914666212019 @default.
• W2091466621 countsByYear W20914666212020 @default.
• W2091466621 countsByYear W20914666212021 @default.
• W2091466621 countsByYear W20914666212022 @default.
• W2091466621 countsByYear W20914666212023 @default.
• W2091466621 crossrefType "journal-article" @default.
• W2091466621 hasAuthorship W2091466621A5002951013 @default.
• W2091466621 hasAuthorship W2091466621A5065404276 @default.
• W2091466621 hasAuthorship W2091466621A5065920375 @default.
• W2091466621 hasBestOaLocation W20914666212 @default.
• W2091466621 hasConcept C112243037 @default.
• W2091466621 hasConcept C150903083 @default.
• W2091466621 hasConcept C178666793 @default.
• W2091466621 hasConcept C181199279 @default.
• W2091466621 hasConcept C185592680 @default.
• W2091466621 hasConcept C202751555 @default.
• W2091466621 hasConcept C207001950 @default.
• W2091466621 hasConcept C28212737 @default.
• W2091466621 hasConcept C41183919 @default.
• W2091466621 hasConcept C55493867 @default.
• W2091466621 hasConcept C56856141 @default.
• W2091466621 hasConcept C62231903 @default.
• W2091466621 hasConcept C86803240 @default.
• W2091466621 hasConceptScore W2091466621C112243037 @default.
• W2091466621 hasConceptScore W2091466621C150903083 @default.
• W2091466621 hasConceptScore W2091466621C178666793 @default.

• next