FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091497997> ?p ?o ?g. }

• W2091497997 endingPage "21" @default.
• W2091497997 startingPage "16" @default.
• W2091497997 abstract "Here we describe a useful method for the site-directed immobilization of proteins with a DNA-binding domain (DNA-BD) on the cognate DNA-coated gold surface for surface plasmon resonance (SPR) imaging analyses. In order to assess the performance of this procedure, we utilized two DNA-BDs, yeast GAL4 DNA-BD, and bacterial LexA DNA-BD. After the immobilization of the cognate double-stranded DNAs (dsDNAs) to a gold chip surface with a monolayer of poly(l-lysine) for sequence-specific DNA–protein interaction, purified recombinant GAL4 DNA-BD:EGFP and LexA DNA-BD:RFP fusion proteins were applied to a dsDNA-spotted gold chip, and were subsequently analyzed using an SPR imaging system. Consequently, the recombinant DNA-binding proteins, GAL4 DNA-BD:EGFP and LexA DNA-BD:RFP, were shown to bind selectively to their cognate DNA sequences on the gold chip. Collectively, our results revealed that sequence-specific dsDNA microarray approach could prove useful in performing the site-directed immobilization of DNA-binding proteins onto a gold thin film in a parallel format, and thereby potentially allowing for the analysis of transcription factor binding profiling as well as for the monitoring of protein–protein interactions between target proteins with DNA-binding domain as a fusion tag and their binding partners." @default.
• W2091497997 created "2016-06-24" @default.
• W2091497997 creator A5007382572 @default.
• W2091497997 creator A5010121437 @default.
• W2091497997 creator A5032638609 @default.
• W2091497997 creator A5045332314 @default.
• W2091497997 creator A5059048952 @default.
• W2091497997 creator A5063910699 @default.
• W2091497997 creator A5087284184 @default.
• W2091497997 creator A5091371771 @default.
• W2091497997 date "2008-05-20" @default.
• W2091497997 modified "2023-10-10" @default.
• W2091497997 title "Directed immobilization of DNA-binding proteins on a cognate DNA-modified chip surface" @default.
• W2091497997 cites W1794259928 @default.
• W2091497997 cites W1969166612 @default.
• W2091497997 cites W1970156673 @default.
• W2091497997 cites W1970585823 @default.
• W2091497997 cites W1971563068 @default.
• W2091497997 cites W1973454596 @default.
• W2091497997 cites W1975712471 @default.
• W2091497997 cites W1988633531 @default.
• W2091497997 cites W1994696054 @default.
• W2091497997 cites W2005654950 @default.
• W2091497997 cites W2026848363 @default.
• W2091497997 cites W2029255421 @default.
• W2091497997 cites W2036619897 @default.
• W2091497997 cites W2063266428 @default.
• W2091497997 cites W2067193961 @default.
• W2091497997 cites W2099202583 @default.
• W2091497997 cites W2118304741 @default.
• W2091497997 cites W2128835512 @default.
• W2091497997 cites W2130636562 @default.
• W2091497997 cites W2148091352 @default.
• W2091497997 doi "https://doi.org/10.1016/j.jbiotec.2008.02.019" @default.
• W2091497997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18395923" @default.
• W2091497997 hasPublicationYear "2008" @default.
• W2091497997 type Work @default.
• W2091497997 sameAs 2091497997 @default.
• W2091497997 citedByCount "22" @default.
• W2091497997 countsByYear W20914979972012 @default.
• W2091497997 countsByYear W20914979972013 @default.
• W2091497997 countsByYear W20914979972014 @default.
• W2091497997 countsByYear W20914979972015 @default.
• W2091497997 countsByYear W20914979972016 @default.
• W2091497997 countsByYear W20914979972017 @default.
• W2091497997 countsByYear W20914979972018 @default.
• W2091497997 countsByYear W20914979972019 @default.
• W2091497997 countsByYear W20914979972021 @default.
• W2091497997 crossrefType "journal-article" @default.
• W2091497997 hasAuthorship W2091497997A5007382572 @default.
• W2091497997 hasAuthorship W2091497997A5010121437 @default.
• W2091497997 hasAuthorship W2091497997A5032638609 @default.
• W2091497997 hasAuthorship W2091497997A5045332314 @default.
• W2091497997 hasAuthorship W2091497997A5059048952 @default.
• W2091497997 hasAuthorship W2091497997A5063910699 @default.
• W2091497997 hasAuthorship W2091497997A5087284184 @default.
• W2091497997 hasAuthorship W2091497997A5091371771 @default.
• W2091497997 hasConcept C101762097 @default.
• W2091497997 hasConcept C104317684 @default.
• W2091497997 hasConcept C106847996 @default.
• W2091497997 hasConcept C123894998 @default.
• W2091497997 hasConcept C150194340 @default.
• W2091497997 hasConcept C153911025 @default.
• W2091497997 hasConcept C155672457 @default.
• W2091497997 hasConcept C158448853 @default.
• W2091497997 hasConcept C171250308 @default.
• W2091497997 hasConcept C185592680 @default.
• W2091497997 hasConcept C192562407 @default.
• W2091497997 hasConcept C32010083 @default.
• W2091497997 hasConcept C33987129 @default.
• W2091497997 hasConcept C3662595 @default.
• W2091497997 hasConcept C40767141 @default.
• W2091497997 hasConcept C413212 @default.
• W2091497997 hasConcept C5179208 @default.
• W2091497997 hasConcept C552990157 @default.
• W2091497997 hasConcept C55493867 @default.
• W2091497997 hasConcept C86339819 @default.
• W2091497997 hasConcept C86803240 @default.
• W2091497997 hasConcept C94966510 @default.
• W2091497997 hasConcept C95371953 @default.
• W2091497997 hasConceptScore W2091497997C101762097 @default.
• W2091497997 hasConceptScore W2091497997C104317684 @default.
• W2091497997 hasConceptScore W2091497997C106847996 @default.
• W2091497997 hasConceptScore W2091497997C123894998 @default.
• W2091497997 hasConceptScore W2091497997C150194340 @default.
• W2091497997 hasConceptScore W2091497997C153911025 @default.
• W2091497997 hasConceptScore W2091497997C155672457 @default.
• W2091497997 hasConceptScore W2091497997C158448853 @default.
• W2091497997 hasConceptScore W2091497997C171250308 @default.
• W2091497997 hasConceptScore W2091497997C185592680 @default.
• W2091497997 hasConceptScore W2091497997C192562407 @default.
• W2091497997 hasConceptScore W2091497997C32010083 @default.
• W2091497997 hasConceptScore W2091497997C33987129 @default.
• W2091497997 hasConceptScore W2091497997C3662595 @default.
• W2091497997 hasConceptScore W2091497997C40767141 @default.
• W2091497997 hasConceptScore W2091497997C413212 @default.
• W2091497997 hasConceptScore W2091497997C5179208 @default.
• W2091497997 hasConceptScore W2091497997C552990157 @default.

• next