Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091510734> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2091510734 abstract "A solid phase radioimmunoassay was developed for detecting the quantity of double-stranded and single-stranded DNA antibodies in patients with systemic lupus erythematosus and other connective tissue diseases. The assay system employs a solid support 96-well, flex-vinyl microtiter plate to which bovine methyl albumin is layered, followed by denatured or native calf thymus DNA. A 1:80 dilution of patients' sera was added to respective wells followed by tritiated high affinity anti-IgG, -IgA, or IgM. Denatured DNA (single-stranded DNA) bound to methylated bovine serum albumin had less than 5% reannealment to the double-stranded form and provided a better substrate for Ab binding than double-stranded DNA, producing a linear binding curve. Of 58 patients diagnosed as having systemic lupus erythematosus (SLE), only 11 having active SLE had IgG antibody levels of greater than 5.0 microgram/ml to single-strand DNA. Renal involvement of some degree was found in all 11 with the high concentrations of IgG antibodies to DNA correlating with severe involvement. Patients with IgM antibodies to DNA alone had more benign types of SLE with little renal involvement. No abnormal levels of IgA Ab to either single-strand DNA or double-strand DNA were found in SLE patients' sera. Corticosteroid and/or immunosuppressant treatment caused a marked drop in the IgM Ab level to DNA within 10 days while IgG Ab to DNA remained high for up to 30 days. Quantitation of IgG and IgM Ab to single-strand DNA provides a useful method for diagnosing severe SLE with possible renal involvement and monitoring the course of the disease during therapy." @default.
- W2091510734 created "2016-06-24" @default.
- W2091510734 creator A5002893700 @default.
- W2091510734 creator A5017569436 @default.
- W2091510734 creator A5062301665 @default.
- W2091510734 creator A5087862216 @default.
- W2091510734 date "1979-02-01" @default.
- W2091510734 modified "2023-09-26" @default.
- W2091510734 title "Significance of Levels of Specific Immunoglobulins to DNA in SLE Patients' Sera Detected by Solid Phase Radioimmunoassay" @default.
- W2091510734 cites W133025572 @default.
- W2091510734 cites W1568220449 @default.
- W2091510734 cites W1592800207 @default.
- W2091510734 cites W1871490883 @default.
- W2091510734 cites W1905837124 @default.
- W2091510734 cites W1918431092 @default.
- W2091510734 cites W1968396257 @default.
- W2091510734 cites W1982587463 @default.
- W2091510734 cites W2014049252 @default.
- W2091510734 cites W2014950129 @default.
- W2091510734 cites W2047009337 @default.
- W2091510734 cites W2056877069 @default.
- W2091510734 cites W2063507221 @default.
- W2091510734 cites W2077498343 @default.
- W2091510734 cites W2090759148 @default.
- W2091510734 cites W2091581918 @default.
- W2091510734 cites W2316085552 @default.
- W2091510734 cites W2414941567 @default.
- W2091510734 cites W2419489441 @default.
- W2091510734 cites W2429983321 @default.
- W2091510734 cites W1792464683 @default.
- W2091510734 doi "https://doi.org/10.1111/1523-1747.ep12530242" @default.
- W2091510734 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/422867" @default.
- W2091510734 hasPublicationYear "1979" @default.
- W2091510734 type Work @default.
- W2091510734 sameAs 2091510734 @default.
- W2091510734 citedByCount "9" @default.
- W2091510734 crossrefType "journal-article" @default.
- W2091510734 hasAuthorship W2091510734A5002893700 @default.
- W2091510734 hasAuthorship W2091510734A5017569436 @default.
- W2091510734 hasAuthorship W2091510734A5062301665 @default.
- W2091510734 hasAuthorship W2091510734A5087862216 @default.
- W2091510734 hasBestOaLocation W20915107341 @default.
- W2091510734 hasConcept C153911025 @default.
- W2091510734 hasConcept C159654299 @default.
- W2091510734 hasConcept C176685330 @default.
- W2091510734 hasConcept C185592680 @default.
- W2091510734 hasConcept C203014093 @default.
- W2091510734 hasConcept C552990157 @default.
- W2091510734 hasConcept C55493867 @default.
- W2091510734 hasConcept C86803240 @default.
- W2091510734 hasConcept C86807702 @default.
- W2091510734 hasConceptScore W2091510734C153911025 @default.
- W2091510734 hasConceptScore W2091510734C159654299 @default.
- W2091510734 hasConceptScore W2091510734C176685330 @default.
- W2091510734 hasConceptScore W2091510734C185592680 @default.
- W2091510734 hasConceptScore W2091510734C203014093 @default.
- W2091510734 hasConceptScore W2091510734C552990157 @default.
- W2091510734 hasConceptScore W2091510734C55493867 @default.
- W2091510734 hasConceptScore W2091510734C86803240 @default.
- W2091510734 hasConceptScore W2091510734C86807702 @default.
- W2091510734 hasLocation W20915107341 @default.
- W2091510734 hasLocation W20915107342 @default.
- W2091510734 hasOpenAccess W2091510734 @default.
- W2091510734 hasPrimaryLocation W20915107341 @default.
- W2091510734 hasRelatedWork W1532638724 @default.
- W2091510734 hasRelatedWork W1680937152 @default.
- W2091510734 hasRelatedWork W1992169430 @default.
- W2091510734 hasRelatedWork W2003278209 @default.
- W2091510734 hasRelatedWork W2046288616 @default.
- W2091510734 hasRelatedWork W2063299633 @default.
- W2091510734 hasRelatedWork W2085963900 @default.
- W2091510734 hasRelatedWork W2207936381 @default.
- W2091510734 hasRelatedWork W2395101467 @default.
- W2091510734 hasRelatedWork W4253483473 @default.
- W2091510734 isParatext "false" @default.
- W2091510734 isRetracted "false" @default.
- W2091510734 magId "2091510734" @default.
- W2091510734 workType "article" @default.