FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091531693> ?p ?o ?g. }

• W2091531693 endingPage "36" @default.
• W2091531693 startingPage "23" @default.
• W2091531693 abstract "The efficacy of cancer immunotherapy can be improved by treatment with full-length tumor antigen and by combining several antigens. This approach allows the induction of a broad immune response irrespective of the patient's HLA type which at the same time challenges immune monitoring. Also, the number of available lymphocytes is most often limited and minimal in vitro restimulations of the lymphocytes should maintain information about the actual in vivo situation. To overcome these hurdles, we developed a method to measure the CD8+ and CD4+ T-cell responses directly ex vivo. Skin biopsies taken from dendritic cell (DC)-induced DTH reactions from melanoma patients participating in a DC-clinical trial served as lymphocyte source. Antigen-specificity of skin infiltrating lymphocytes was investigated by coculture with antigen-presenting autologous B cells and assessed for CD137 upregulation and enhanced cytokine secretion. Using this approach we could detect treatment-specific CD8+ T-cells without restimulation in vitro. Upregulation of the activation marker CD137 correlated with the upregulation of the lytic marker CD107a. CD137 upregulation by treatment-specific CD4+ lymphocytes however was more pronounced after antigen-specific in vitro restimulation. Both CD8+ and CD4+ lymphocytes could be further expanded using the same B cells as for screening allowing characterization of the recognized antigenic region. In addition, this technique can be extended to detect a broader array of T-cell functions and to monitor a large cohort of patients. We believe that this approach of direct ex vivo monitoring, irrespective of the patient's HLA-type or the recognized peptide, and using a limited number of lymphocytes is a valuable tool in the immune monitoring of current cellular immunotherapies." @default.
• W2091531693 created "2016-06-24" @default.
• W2091531693 creator A5033241663 @default.
• W2091531693 creator A5034808697 @default.
• W2091531693 creator A5042550529 @default.
• W2091531693 creator A5046458252 @default.
• W2091531693 creator A5048170721 @default.
• W2091531693 creator A5070959446 @default.
• W2091531693 creator A5075569536 @default.
• W2091531693 creator A5077888286 @default.
• W2091531693 creator A5082449165 @default.
• W2091531693 date "2012-03-01" @default.
• W2091531693 modified "2023-09-23" @default.
• W2091531693 title "Epitope and HLA-type independent monitoring of antigen-specific T-cells after treatment with dendritic cells presenting full-length tumor antigens" @default.
• W2091531693 cites W1494281090 @default.
• W2091531693 cites W1515298765 @default.
• W2091531693 cites W1521418395 @default.
• W2091531693 cites W1569979927 @default.
• W2091531693 cites W1939433055 @default.
• W2091531693 cites W1967684158 @default.
• W2091531693 cites W1969256234 @default.
• W2091531693 cites W1969475094 @default.
• W2091531693 cites W1985278235 @default.
• W2091531693 cites W1994023904 @default.
• W2091531693 cites W1994971348 @default.
• W2091531693 cites W1999502843 @default.
• W2091531693 cites W2004020904 @default.
• W2091531693 cites W2004037261 @default.
• W2091531693 cites W2008783725 @default.
• W2091531693 cites W2017631687 @default.
• W2091531693 cites W2024794399 @default.
• W2091531693 cites W2052012700 @default.
• W2091531693 cites W2059467017 @default.
• W2091531693 cites W2063667712 @default.
• W2091531693 cites W2068034404 @default.
• W2091531693 cites W2070177252 @default.
• W2091531693 cites W2070668733 @default.
• W2091531693 cites W2072723772 @default.
• W2091531693 cites W2074572506 @default.
• W2091531693 cites W2089401318 @default.
• W2091531693 cites W2090866819 @default.
• W2091531693 cites W2096554723 @default.
• W2091531693 cites W2103291317 @default.
• W2091531693 cites W2103959341 @default.
• W2091531693 cites W2117077226 @default.
• W2091531693 cites W2117503715 @default.
• W2091531693 cites W2124034595 @default.
• W2091531693 cites W2125416460 @default.
• W2091531693 cites W2133866634 @default.
• W2091531693 cites W2135087025 @default.
• W2091531693 cites W2135288013 @default.
• W2091531693 cites W2138064172 @default.
• W2091531693 cites W2139517175 @default.
• W2091531693 cites W2144797760 @default.
• W2091531693 cites W2152093797 @default.
• W2091531693 cites W2156253561 @default.
• W2091531693 cites W2170955899 @default.
• W2091531693 cites W2327804995 @default.
• W2091531693 cites W2496378471 @default.
• W2091531693 cites W4235084121 @default.
• W2091531693 doi "https://doi.org/10.1016/j.jim.2011.12.010" @default.
• W2091531693 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22269772" @default.
• W2091531693 hasPublicationYear "2012" @default.
• W2091531693 type Work @default.
• W2091531693 sameAs 2091531693 @default.
• W2091531693 citedByCount "24" @default.
• W2091531693 countsByYear W20915316932012 @default.
• W2091531693 countsByYear W20915316932013 @default.
• W2091531693 countsByYear W20915316932014 @default.
• W2091531693 countsByYear W20915316932015 @default.
• W2091531693 countsByYear W20915316932016 @default.
• W2091531693 countsByYear W20915316932017 @default.
• W2091531693 countsByYear W20915316932020 @default.
• W2091531693 crossrefType "journal-article" @default.
• W2091531693 hasAuthorship W2091531693A5033241663 @default.
• W2091531693 hasAuthorship W2091531693A5034808697 @default.
• W2091531693 hasAuthorship W2091531693A5042550529 @default.
• W2091531693 hasAuthorship W2091531693A5046458252 @default.
• W2091531693 hasAuthorship W2091531693A5048170721 @default.
• W2091531693 hasAuthorship W2091531693A5070959446 @default.
• W2091531693 hasAuthorship W2091531693A5075569536 @default.
• W2091531693 hasAuthorship W2091531693A5077888286 @default.
• W2091531693 hasAuthorship W2091531693A5082449165 @default.
• W2091531693 hasConcept C104317684 @default.
• W2091531693 hasConcept C127561419 @default.
• W2091531693 hasConcept C147483822 @default.
• W2091531693 hasConcept C150903083 @default.
• W2091531693 hasConcept C154317977 @default.
• W2091531693 hasConcept C167672396 @default.
• W2091531693 hasConcept C202751555 @default.
• W2091531693 hasConcept C203014093 @default.
• W2091531693 hasConcept C207001950 @default.
• W2091531693 hasConcept C26291073 @default.
• W2091531693 hasConcept C2776090121 @default.
• W2091531693 hasConcept C2777701055 @default.
• W2091531693 hasConcept C2780249625 @default.
• W2091531693 hasConcept C502942594 @default.
• W2091531693 hasConcept C55493867 @default.

• next