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- W2091539999 abstract "A radioimmunoassay for the measurement of prednisone in serum has been developed. The method is accurate, precise, specific, and very sensitive. Using a primary antibody elicited against prednisone 21-hemisuccinate-bovine serum albumin and a chemical precipitation step, the assay is capable of detecting 6.25 picograms of prednisone in 0.1 ml of unextracted diluted serum or plasma. The specificity of the assay is influenced by the carbonyl groups at position 11 and 20, and the double bond at the 1-position of the steroid nucleus. Physiological levels of endogenous steroid interfered only slightly with the primary antibody. Measurement of serum concentrations of prednisone in man and dog were accomplished following the administration of prednisone (Deltasone®). This assay, used in conjunction with a published radioimmunoassay for prednisolone (1), can be used to determine the interconversion of these two drug products following prednisone or prednisolone administration." @default.
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- W2091539999 date "1974-07-01" @default.
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- W2091539999 title "Radioimmunoassay for prednisone" @default.
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- W2091539999 doi "https://doi.org/10.1016/0039-128x(74)90048-8" @default.
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