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• W2091567124 abstract "The T cell receptor (TCR) determines the cellular response to antigens, which are presented on the surface of target cells in the form of a peptide bound to a product of the major histocompatibility complex (pepMHC). The response of the T cell depends on the affinity of the TCR for the pepMHC, yet many TCRs have been shown to be of low affinity, and some naturally occurring T cell responses are poor due to low affinities. Accordingly, engineering the TCR for increased affinity for pepMHC, particularly tumor-associated antigens, has become an increasingly desirable goal, especially with the advent of adoptive T cell therapies. For largely technical reasons, to date there have been only a handful of TCRs engineered in vitro for higher affinity using well established methods of protein engineering. Here we report the use of a T cell display system, using a retroviral vector, for generating a high-affinity TCR from the mouse T cell clone 2C. The method relies on the display of the TCR, in its normal, signaling competent state, as a CD3 complex on the T cell surface. A library in the CDR3alpha of the 2C TCR was generated in the MSCV retroviral vector and transduced into a TCR-negative hybridoma. Selection of a high-affinity, CD8-independent TCR was accomplished after only two rounds of flow cytometric sorting using the pepMHC SIYRYYGL/Kb (SIY/Kb). The selected TCR contained a sequence motif in the CDR3alpha with characteristics of several other TCRs previously selected by yeast display. In addition, it was possible to directly use the selected T cell hybridoma in functional assays without the need for sub-cloning, revealing that the selected TCR was capable of mediating CD8-independent activity. The method may be useful in the direct isolation and characterization of TCRs that could be used in therapies with adoptive transferred T cells." @default.
• W2091567124 created "2016-06-24" @default.
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• W2091567124 creator A5067522153 @default.
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• W2091567124 date "2008-12-01" @default.
• W2091567124 modified "2023-10-18" @default.
• W2091567124 title "Engineering higher affinity T cell receptors using a T cell display system" @default.
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• W2091567124 cites W1933637003 @default.
• W2091567124 cites W1963704859 @default.
• W2091567124 cites W1969797226 @default.
• W2091567124 cites W1971596340 @default.
• W2091567124 cites W1974559250 @default.
• W2091567124 cites W1979971440 @default.
• W2091567124 cites W1983412131 @default.
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• W2091567124 cites W2007439292 @default.
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• W2091567124 cites W2020012742 @default.
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• W2091567124 doi "https://doi.org/10.1016/j.jim.2008.09.016" @default.
• W2091567124 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2680719" @default.
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