FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091569473> ?p ?o ?g. }

• W2091569473 endingPage "2575" @default.
• W2091569473 startingPage "2569" @default.
• W2091569473 abstract "Frog alpha-macroglobulin was purified to apparent homogeneity by Ni2+ chelate affinity chromatography. Frog alpha-macroglobulin migrated as an alpha 1-globulin in cellulose acetate electrophoresis. A molecular weight of 730 000 was obtained by equilibrium sedimentation, and in sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), the protein migrated as a single band of Mr approximately 360 000 before reduction and Mr approximately 180 000 after reduction. Treatment with trypsin resulted in subunit cleavage to yield a fragment of Mr approximately 90 000. After being heated, the protein fragmented, migrating in SDS-PAGE as two bands of Mr approximately 120 000 and 60 000. This fragmentation was inhibited by prior reaction of the protein with methylamine. In native pore-limit electrophoresis the protein exhibited the characteristic slow to fast conformational change of protease-treated alpha-macroglobulins. In contrast, typical slow to fast conformational change was not observed in native PAGE with this preparation. Moreover, the protein incorporated approximately 2 mol of [14C]methylamine/mol of inhibitor without demonstrating a change in mobility in native PAGE. In circular dichroism studies, the protein exhibited a spectrum similar to that of human alpha 2M. Reaction with trypsin resulted in a broadening and decrease in the magnitude of the spectrum. Reaction with methylamine resulted in similar changes, but of smaller magnitude. The inhibitor bound approximately 0.7 mol of trypsin in both radiolabeled protease binding and amidolytic titration studies. 125I-Labeled native frog alpha 1M was removed slowly from the circulation of mice with a t1/2 greater than 2h.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W2091569473 created "2016-06-24" @default.
• W2091569473 creator A5006964107 @default.
• W2091569473 creator A5090928660 @default.
• W2091569473 date "1985-05-07" @default.
• W2091569473 modified "2023-09-26" @default.
• W2091569473 title "Purification and characterization of frog .alpha.-macroglobulin: receptor recognition of an amphibian glycoprotein" @default.
• W2091569473 cites W1461662986 @default.
• W2091569473 cites W1470416536 @default.
• W2091569473 cites W1514143259 @default.
• W2091569473 cites W1516050685 @default.
• W2091569473 cites W1519994409 @default.
• W2091569473 cites W1527563327 @default.
• W2091569473 cites W1529295649 @default.
• W2091569473 cites W154570453 @default.
• W2091569473 cites W1580023435 @default.
• W2091569473 cites W1582833331 @default.
• W2091569473 cites W1608191411 @default.
• W2091569473 cites W1625660792 @default.
• W2091569473 cites W1631168844 @default.
• W2091569473 cites W1720735718 @default.
• W2091569473 cites W1758096854 @default.
• W2091569473 cites W1775749144 @default.
• W2091569473 cites W1790158792 @default.
• W2091569473 cites W1845041229 @default.
• W2091569473 cites W1919809459 @default.
• W2091569473 cites W1952851700 @default.
• W2091569473 cites W1953834341 @default.
• W2091569473 cites W1968834637 @default.
• W2091569473 cites W1971865056 @default.
• W2091569473 cites W1975299458 @default.
• W2091569473 cites W1981874508 @default.
• W2091569473 cites W1996643248 @default.
• W2091569473 cites W2001621831 @default.
• W2091569473 cites W2003297361 @default.
• W2091569473 cites W2012839797 @default.
• W2091569473 cites W2019524205 @default.
• W2091569473 cites W2027041860 @default.
• W2091569473 cites W2028759964 @default.
• W2091569473 cites W2030905982 @default.
• W2091569473 cites W2031853505 @default.
• W2091569473 cites W2033556591 @default.
• W2091569473 cites W2036212754 @default.
• W2091569473 cites W2044914819 @default.
• W2091569473 cites W2047903470 @default.
• W2091569473 cites W2054941870 @default.
• W2091569473 cites W2068037889 @default.
• W2091569473 cites W2068934482 @default.
• W2091569473 cites W2072609742 @default.
• W2091569473 cites W2074631079 @default.
• W2091569473 cites W2074799652 @default.
• W2091569473 cites W2095029703 @default.
• W2091569473 cites W2105598359 @default.
• W2091569473 cites W2119460480 @default.
• W2091569473 cites W2125748383 @default.
• W2091569473 cites W2137391369 @default.
• W2091569473 cites W2220760489 @default.
• W2091569473 cites W2329890782 @default.
• W2091569473 cites W2344409942 @default.
• W2091569473 cites W2401830315 @default.
• W2091569473 cites W99799268 @default.
• W2091569473 doi "https://doi.org/10.1021/bi00331a026" @default.
• W2091569473 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2410015" @default.
• W2091569473 hasPublicationYear "1985" @default.
• W2091569473 type Work @default.
• W2091569473 sameAs 2091569473 @default.
• W2091569473 citedByCount "25" @default.
• W2091569473 crossrefType "journal-article" @default.
• W2091569473 hasAuthorship W2091569473A5006964107 @default.
• W2091569473 hasAuthorship W2091569473A5090928660 @default.
• W2091569473 hasConcept C181199279 @default.
• W2091569473 hasConcept C185592680 @default.
• W2091569473 hasConcept C2776714187 @default.
• W2091569473 hasConcept C2778969155 @default.
• W2091569473 hasConcept C2780340462 @default.
• W2091569473 hasConcept C2781054842 @default.
• W2091569473 hasConcept C43617362 @default.
• W2091569473 hasConcept C52543561 @default.
• W2091569473 hasConcept C55493867 @default.
• W2091569473 hasConcept C71240020 @default.
• W2091569473 hasConceptScore W2091569473C181199279 @default.
• W2091569473 hasConceptScore W2091569473C185592680 @default.
• W2091569473 hasConceptScore W2091569473C2776714187 @default.
• W2091569473 hasConceptScore W2091569473C2778969155 @default.
• W2091569473 hasConceptScore W2091569473C2780340462 @default.
• W2091569473 hasConceptScore W2091569473C2781054842 @default.
• W2091569473 hasConceptScore W2091569473C43617362 @default.
• W2091569473 hasConceptScore W2091569473C52543561 @default.
• W2091569473 hasConceptScore W2091569473C55493867 @default.
• W2091569473 hasConceptScore W2091569473C71240020 @default.
• W2091569473 hasIssue "10" @default.
• W2091569473 hasLocation W20915694731 @default.
• W2091569473 hasLocation W20915694732 @default.
• W2091569473 hasOpenAccess W2091569473 @default.
• W2091569473 hasPrimaryLocation W20915694731 @default.
• W2091569473 hasRelatedWork W105318187 @default.
• W2091569473 hasRelatedWork W1716222573 @default.
• W2091569473 hasRelatedWork W1762439951 @default.

• next