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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091569698> ?p ?o ?g. }

• W2091569698 endingPage "2046" @default.
• W2091569698 startingPage "2041" @default.
• W2091569698 abstract "ABSTRACT Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10 1 to 10 6 copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China." @default.
• W2091569698 created "2016-06-24" @default.
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• W2091569698 date "2005-05-01" @default.
• W2091569698 modified "2023-10-11" @default.
• W2091569698 title "Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets" @default.
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• W2091569698 doi "https://doi.org/10.1128/jcm.43.5.2041-2046.2005" @default.
• W2091569698 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1153763" @default.
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