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• W2091628426 abstract "Nucleotide insertion/deletion polymorphisms (indels) in ApoE gene were precisely genotyped using artificial ribonucleases and MALDI-TOF MS. The RNA fragments for MS analysis were prepared by treating RNA specimens with our artificial ribonucleases, which consist of LuCl3 (molecular scissors) and oligonucleotides bearing two acridine groups (RNA-activator for site-selective scission). RNA scission by Lu(III) ion always occurred at the phosphodiester linkages in front of the two acridines, even when the RNA specimens involved consecutive cytidine sequences of different lengths. Thus, even complicated mixtures of these indel specimens were completely genotyped by using only one acridine-bearing oligonucleotide and by subjecting the reaction mixture to single MS measurement. Moreover, single nucleotide polymorphism (SNP) in the consecutive sequences could be genotyped simultaneously with the indels." @default.
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• W2091628426 date "2006-01-01" @default.
• W2091628426 modified "2023-10-03" @default.
• W2091628426 title "Simultaneous genotyping of indels and SNPs by mass spectroscopy" @default.
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• W2091628426 doi "https://doi.org/10.1016/j.jasms.2005.08.016" @default.
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