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- W2091650308 abstract "1. Ox brain triphosphoinositide was incubated with a solubilized preparation of ox brain phosphoinositide phosphodiesterase and at different time intervals the fatty acid compositions of the hydrolysis product, diglyceride, and the residual substrate were examined. The fatty acid compositions of both fractions at all times were essentially constant indicating no high degree of selectivity of particular molecular species of triphosphoinositide by the enzyme. Since the liberated diglycerides largely reflected the original composition of the substrate, it was concluded that the major product of phosphoinositide hydrolysis was 1-stearoyl-2-arachidonoyl-sn-glycerol. 2. Mono-, di- and triphosphoinositides from rat brain were rich in stearate and arachidonate, which were in equimolar proportions and accounted for about 80% of the total fatty acids. Similarly, the total diglycerides of rat brain contained about 30% each of stearate and arachidonate, while, in contrast, mono- and triglycerides had very little arachidonate. 3. The rat brain diglycerides were acetylated and separated and quantitated by argentation-thin-layer chromatography and gas-liquid chromatography. The tetraene species greatly predominated and consisted largely of stearate paired with arachidonate. Hydrolysis of rat brain diglycerides with pancreatic lipase showed that arachidonate was located in the 2-position and stearate mostly in the 1(3)-position. The diglycerides were converted to phosphatidylphenols, which were hydrolysed with snake venom phospholipase A2. About 90% of the phosphatidylphenols were converted to lysophosphatidylphenols and all the arachidonate was liberated as free fatty acid. The collective evidence suggests that almost all the brain diglyceride is 1,2-diacyl-sn-glycerol and that 1-stearoyl-2-arachidonoyl-sn-glycerol accounts for about 60% of this fraction. 4. The possibility is raised that the pool of arachidonoyl diglyceride in brain may be derived, in part, from hydrolysis of phosphoinositides by the phospho-diesterase." @default.
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- W2091650308 date "1972-07-01" @default.
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- W2091650308 title "A possible relation between phosphoinositides and the diglyceride pool in rat brain" @default.
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- W2091650308 doi "https://doi.org/10.1016/0005-2760(72)90198-1" @default.
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