Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091716002> ?p ?o ?g. }
- W2091716002 endingPage "490" @default.
- W2091716002 startingPage "483" @default.
- W2091716002 abstract "Abnormal tissue factor (TF) expression on vascular endothelial cells may account for thrombotic events associated with cardiovascular disease. The transcription factor nuclear factor-κB (NF-κB) activation plays a key role in endothelial cell injury and TF expression. Disruption of NF-κB activation in endothelial cells may inhibit TF expression and be protective in thrombosis. The purpose of the study was to determine whether NF-κB transcription factor decoy (TFD) could block TF expression. NF-κB TFD was transferred into cultured human umbilical cord vein endothelial cells (HUVEC) by liposomes, and the transfection efficiency was detected by flow cytometry. The effect of NF-κB TFD on TF mRNA levels was determined by reverse transcription-polymerase chain reaction. The expression of surface TF antigen was analyzed by flow cytometry. TF activity was studied by measuring enzymatic activation of factor X by the TF–activated factor VII complex. The results suggested that NF-κB decoy could be successfully transferred into HUVEC by liposome. The NF-κB TFD competed with the endogenous κB site sequence in the TF promoter for binding to transcription factor NF-κB in tumor necrosis factor-α-stimulated HUVEC, which could block the tumor necrosis factor-α-induced increase in TF mRNA levels, the upregulation of surface TF antigen and TF activity. This study demonstrated that NF-κB decoy could block HUVEC TF gene expression. Targeted genetic disruption of endothelial TF expression by NF-κB decoy may provide a possible therapeutic method for cardiovascular and thrombosis disease." @default.
- W2091716002 created "2016-06-24" @default.
- W2091716002 creator A5017397726 @default.
- W2091716002 creator A5019707913 @default.
- W2091716002 creator A5027780282 @default.
- W2091716002 creator A5047359455 @default.
- W2091716002 creator A5080506792 @default.
- W2091716002 date "2004-08-01" @default.
- W2091716002 modified "2023-10-01" @default.
- W2091716002 title "An oligonucleotide decoy for nuclear factor-κB inhibits tumor necrosis factor-α-induced human umbilical cord vein endothelial cell tissue factor expression in vitro" @default.
- W2091716002 cites W1486918734 @default.
- W2091716002 cites W156329827 @default.
- W2091716002 cites W1588051837 @default.
- W2091716002 cites W1860367266 @default.
- W2091716002 cites W1994144487 @default.
- W2091716002 cites W1998640392 @default.
- W2091716002 cites W2029602474 @default.
- W2091716002 cites W2038197749 @default.
- W2091716002 cites W2076042509 @default.
- W2091716002 cites W2079925739 @default.
- W2091716002 cites W2082301173 @default.
- W2091716002 cites W2126762495 @default.
- W2091716002 cites W2142840964 @default.
- W2091716002 cites W2157603347 @default.
- W2091716002 cites W2168592519 @default.
- W2091716002 cites W2169312205 @default.
- W2091716002 cites W2340008042 @default.
- W2091716002 cites W2400229313 @default.
- W2091716002 cites W3194355700 @default.
- W2091716002 cites W4251527986 @default.
- W2091716002 cites W4361865076 @default.
- W2091716002 cites W1512144932 @default.
- W2091716002 doi "https://doi.org/10.1097/00001721-200408000-00007" @default.
- W2091716002 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15311157" @default.
- W2091716002 hasPublicationYear "2004" @default.
- W2091716002 type Work @default.
- W2091716002 sameAs 2091716002 @default.
- W2091716002 citedByCount "6" @default.
- W2091716002 countsByYear W20917160022013 @default.
- W2091716002 countsByYear W20917160022021 @default.
- W2091716002 crossrefType "journal-article" @default.
- W2091716002 hasAuthorship W2091716002A5017397726 @default.
- W2091716002 hasAuthorship W2091716002A5019707913 @default.
- W2091716002 hasAuthorship W2091716002A5027780282 @default.
- W2091716002 hasAuthorship W2091716002A5047359455 @default.
- W2091716002 hasAuthorship W2091716002A5080506792 @default.
- W2091716002 hasConcept C104317684 @default.
- W2091716002 hasConcept C123012128 @default.
- W2091716002 hasConcept C126322002 @default.
- W2091716002 hasConcept C153911025 @default.
- W2091716002 hasConcept C170493617 @default.
- W2091716002 hasConcept C17991360 @default.
- W2091716002 hasConcept C186738567 @default.
- W2091716002 hasConcept C202751555 @default.
- W2091716002 hasConcept C203014093 @default.
- W2091716002 hasConcept C2777411675 @default.
- W2091716002 hasConcept C2778382381 @default.
- W2091716002 hasConcept C2779179475 @default.
- W2091716002 hasConcept C502942594 @default.
- W2091716002 hasConcept C553184892 @default.
- W2091716002 hasConcept C55493867 @default.
- W2091716002 hasConcept C71924100 @default.
- W2091716002 hasConcept C86339819 @default.
- W2091716002 hasConcept C86803240 @default.
- W2091716002 hasConceptScore W2091716002C104317684 @default.
- W2091716002 hasConceptScore W2091716002C123012128 @default.
- W2091716002 hasConceptScore W2091716002C126322002 @default.
- W2091716002 hasConceptScore W2091716002C153911025 @default.
- W2091716002 hasConceptScore W2091716002C170493617 @default.
- W2091716002 hasConceptScore W2091716002C17991360 @default.
- W2091716002 hasConceptScore W2091716002C186738567 @default.
- W2091716002 hasConceptScore W2091716002C202751555 @default.
- W2091716002 hasConceptScore W2091716002C203014093 @default.
- W2091716002 hasConceptScore W2091716002C2777411675 @default.
- W2091716002 hasConceptScore W2091716002C2778382381 @default.
- W2091716002 hasConceptScore W2091716002C2779179475 @default.
- W2091716002 hasConceptScore W2091716002C502942594 @default.
- W2091716002 hasConceptScore W2091716002C553184892 @default.
- W2091716002 hasConceptScore W2091716002C55493867 @default.
- W2091716002 hasConceptScore W2091716002C71924100 @default.
- W2091716002 hasConceptScore W2091716002C86339819 @default.
- W2091716002 hasConceptScore W2091716002C86803240 @default.
- W2091716002 hasIssue "6" @default.
- W2091716002 hasLocation W20917160021 @default.
- W2091716002 hasLocation W20917160022 @default.
- W2091716002 hasOpenAccess W2091716002 @default.
- W2091716002 hasPrimaryLocation W20917160021 @default.
- W2091716002 hasRelatedWork W1483760162 @default.
- W2091716002 hasRelatedWork W2012048925 @default.
- W2091716002 hasRelatedWork W2023521016 @default.
- W2091716002 hasRelatedWork W2062115483 @default.
- W2091716002 hasRelatedWork W2256945679 @default.
- W2091716002 hasRelatedWork W2380549790 @default.
- W2091716002 hasRelatedWork W2460292361 @default.
- W2091716002 hasRelatedWork W4231498531 @default.
- W2091716002 hasRelatedWork W4232984710 @default.
- W2091716002 hasRelatedWork W4254280018 @default.
- W2091716002 hasVolume "15" @default.