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- W2091778526 abstract "A cleanup method based on anion-exchange solid-phase extraction (SPE) was developed to render biological extracts suitable for the analysis of hexose phosphates with a modified anion-exchange chromatography method and pulsed amperometric detection. The method was applied to cell extracts ofSaccharomyces cerevisiaeobtained by using cold methanol as quenching agent and chloroform as extraction solvent. It was shown that pretreatment of the cell extract with SPE markedly improved the quality of the liquid chromatography analysis with recoveries of the sugar phosphates close to 100%. Furthermore, the method allowed for sample enrichment and the original extraction procedure could be simplified by implementing SPE early in the extraction protocol." @default.
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- W2091778526 date "1998-07-01" @default.
- W2091778526 modified "2023-10-01" @default.
- W2091778526 title "Cleanup and Analysis of Sugar Phosphates in Biological Extracts by Using Solid-Phase Extraction and Anion-Exchange Chromatography with Pulsed Amperometric Detection" @default.
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- W2091778526 doi "https://doi.org/10.1006/abio.1998.2714" @default.
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