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- W2091784900 abstract "We developed a novel protein complex isolation method using a single-chain variable fragment (scFv) based phage display library in a two-step purification procedure. We adapted the antibody-based phage display technology which has been developed for single target proteins to a protein mixture containing about 300 proteins, mostly subunits of Thermoplasma acidophilum complexes. T. acidophilum protein specific phages were selected and corresponding scFvs were expressed in Escherichia coli. E. coli cell lysate containing the expressed His-tagged scFv specific against one antigen protein and T. acidophilum crude cell lysate containing intact target protein complexes were mixed, incubated and subjected to protein purification using affinity and size exclusion chromatography steps. This method was confirmed to isolate intact particles of thermosome and proteasome suitable for electron microscopy analysis and provides a novel protein complex isolation strategy applicable to organisms where no genetic tools are available." @default.
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- W2091784900 date "2014-03-01" @default.
- W2091784900 modified "2023-09-27" @default.
- W2091784900 title "Protein complex purification from Thermoplasma acidophilum using a phage display library" @default.
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- W2091784900 doi "https://doi.org/10.1016/j.mimet.2013.12.010" @default.
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