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- W2091796104 abstract "The development of pycnotic nuclei in irradiated thymus cells during incubation at 37 °C in vitro is minimal at low pH values and is accelerated at high pH values. In contrast, the development of other signs of cell degeneration, i.e., loss of intracellular potassium and absorption of erythrosin stain by intact cells, was minimal at pH values near 6.2 and was accelerated either at lower or at higher pH values. Pycnosis at pH 7.5 was also prevented by respiratory inhibitors, e.g. dinitrophenol, cyanide, arsenite, iodoacetamide, high doses of X-radiation, or cationic condensing agents. From these results, it was concluded that pycnosis represents a physical dispersion of nuclear components which can be prevented either by treatments which irreversibly denature the nuclear proteins or by treatments which cause a reversible gelation of the nucleoplasm due to increased acidity. The lysis of cell nuclei is reportedly inhibited by the same treatments [26]. The absence of nuclear pycnosis does not necessarily indicate absence of cell damage. In most but not all of the experimental conditions tested, potassium loss was correlated with erythrosin staining but it developed at an earlier stage of cell degeneration. Of the three criteria, the loss of intracellular potassium appeared to provide the most reliable index of damage to the thymus cells." @default.
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- W2091796104 date "1965-05-01" @default.
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- W2091796104 title "Prevention of pycnosis in rat thymocytes" @default.
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- W2091796104 doi "https://doi.org/10.1016/0014-4827(65)90409-x" @default.
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