FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091836249> ?p ?o ?g. }

• W2091836249 endingPage "287" @default.
• W2091836249 startingPage "277" @default.
• W2091836249 abstract "The aim of the present study was to investigate the role of the entitled neutral, sphingomyelinase in the non-lysosomal pathway of sphingomyelin degradation by intact cells (Spence et al. (1983) J. Biol. Chem. 258, 8595-8600; Levade et al. (1991) J. Biol. Chem. 266, 13519-13529). The uptake and degradation of sphingomyelin by intact living cells was studied using cell lines exhibiting a wide range of activity levels of acid, lysosomal and neutral sphingomyelinases as determined in vitro on cell homogenates by their respective standard assays. For this purpose, neuroblastoma, skin fibroblasts, lymphoid and leukemic cell lines, some of them derived from patients with Niemann-Pick disease (deficient in the acid, lysosomal sphingomyelinase) were incubated with radioactive, [oleoyl-3H]sphingomyelin or fluorescent, pyrene-sulfonylaminoundecanoyl-sphingomyelin. Either compound was taken up by a pathway which was not receptor-mediated and hydrolyzed by all intact cells, including those derived from Niemann-Pick disease patients. Moreover, their degradation by the intact cells was not inhibited by treatment with chloroquine, indicating hydrolysis by a non-lysosomal sphingomyelinase. The intracellular sphingomyelin degradation rates showed no correlation with the activity of the 'classical' neutral sphingomyelinase as determined in vitro. In particular, fibroblasts derived from Niemann-Pick patients lacking the lysosomal sphingomyelinase, and having no detectable in vitro activity of the 'classical' neutral sphingomyelinase, were able to degrade the exogenously supplied sphingomyelins. Indeed, in vitro these cells were shown to exhibit neutral, magnesium- and dithiothreitol-dependent sphingomyelinase activities, that might contribute to the non-lysosomal pathway for sphingomyelin degradation to ceramide in intact cells." @default.
• W2091836249 created "2016-06-24" @default.
• W2091836249 creator A5009713793 @default.
• W2091836249 creator A5011401228 @default.
• W2091836249 creator A5040785436 @default.
• W2091836249 creator A5073545182 @default.
• W2091836249 creator A5074211447 @default.
• W2091836249 creator A5076590438 @default.
• W2091836249 date "1995-10-01" @default.
• W2091836249 modified "2023-09-27" @default.
• W2091836249 title "Degradation of fluorescent and radiolabelled sphingomyelins in intact cells by a non-lysosomal pathway" @default.
• W2091836249 cites W1423470542 @default.
• W2091836249 cites W1491611834 @default.
• W2091836249 cites W1493153573 @default.
• W2091836249 cites W1497397052 @default.
• W2091836249 cites W1513792874 @default.
• W2091836249 cites W1542941723 @default.
• W2091836249 cites W1544997431 @default.
• W2091836249 cites W1557233561 @default.
• W2091836249 cites W1563888013 @default.
• W2091836249 cites W1566441785 @default.
• W2091836249 cites W1572050650 @default.
• W2091836249 cites W1577542502 @default.
• W2091836249 cites W1577946620 @default.
• W2091836249 cites W1578044243 @default.
• W2091836249 cites W1605902541 @default.
• W2091836249 cites W1607687470 @default.
• W2091836249 cites W1635474458 @default.
• W2091836249 cites W1680920356 @default.
• W2091836249 cites W1823818251 @default.
• W2091836249 cites W1912410317 @default.
• W2091836249 cites W1964967794 @default.
• W2091836249 cites W1965651743 @default.
• W2091836249 cites W1982975958 @default.
• W2091836249 cites W1986841583 @default.
• W2091836249 cites W1993950559 @default.
• W2091836249 cites W2000823512 @default.
• W2091836249 cites W2006721039 @default.
• W2091836249 cites W2009263073 @default.
• W2091836249 cites W2009316968 @default.
• W2091836249 cites W200967821 @default.
• W2091836249 cites W2009993180 @default.
• W2091836249 cites W2014664200 @default.
• W2091836249 cites W2017393429 @default.
• W2091836249 cites W2017729786 @default.
• W2091836249 cites W2018716777 @default.
• W2091836249 cites W2037628996 @default.
• W2091836249 cites W2041576624 @default.
• W2091836249 cites W2048453877 @default.
• W2091836249 cites W2049108649 @default.
• W2091836249 cites W2049165095 @default.
• W2091836249 cites W2051061198 @default.
• W2091836249 cites W2053858732 @default.
• W2091836249 cites W2055041264 @default.
• W2091836249 cites W2056500157 @default.
• W2091836249 cites W2061574494 @default.
• W2091836249 cites W2069370714 @default.
• W2091836249 cites W2070005836 @default.
• W2091836249 cites W2076889165 @default.
• W2091836249 cites W2080071635 @default.
• W2091836249 cites W2085364060 @default.
• W2091836249 cites W2091536410 @default.
• W2091836249 cites W2092330976 @default.
• W2091836249 cites W2116716030 @default.
• W2091836249 cites W2130706989 @default.
• W2091836249 cites W2147278939 @default.
• W2091836249 cites W2157978273 @default.
• W2091836249 cites W2168526937 @default.
• W2091836249 cites W2253042815 @default.
• W2091836249 cites W2342086544 @default.
• W2091836249 cites W2342329062 @default.
• W2091836249 cites W4232534952 @default.
• W2091836249 cites W4236874274 @default.
• W2091836249 cites W965722434 @default.
• W2091836249 doi "https://doi.org/10.1016/0005-2760(95)00132-v" @default.
• W2091836249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7548198" @default.
• W2091836249 hasPublicationYear "1995" @default.
• W2091836249 type Work @default.
• W2091836249 sameAs 2091836249 @default.
• W2091836249 citedByCount "16" @default.
• W2091836249 countsByYear W20918362492013 @default.
• W2091836249 crossrefType "journal-article" @default.
• W2091836249 hasAuthorship W2091836249A5009713793 @default.
• W2091836249 hasAuthorship W2091836249A5011401228 @default.
• W2091836249 hasAuthorship W2091836249A5040785436 @default.
• W2091836249 hasAuthorship W2091836249A5073545182 @default.
• W2091836249 hasAuthorship W2091836249A5074211447 @default.
• W2091836249 hasAuthorship W2091836249A5076590438 @default.
• W2091836249 hasConcept C181199279 @default.
• W2091836249 hasConcept C185592680 @default.
• W2091836249 hasConcept C190283241 @default.
• W2091836249 hasConcept C202751555 @default.
• W2091836249 hasConcept C2777851122 @default.
• W2091836249 hasConcept C2778163477 @default.
• W2091836249 hasConcept C2779489717 @default.
• W2091836249 hasConcept C2780216420 @default.
• W2091836249 hasConcept C2781070647 @default.
• W2091836249 hasConcept C2781457462 @default.

• next