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• W2091938644 endingPage "11121" @default.
• W2091938644 startingPage "11117" @default.
• W2091938644 abstract "Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes." @default.
• W2091938644 created "2016-06-24" @default.
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• W2091938644 date "1999-09-28" @default.
• W2091938644 modified "2023-10-18" @default.
• W2091938644 title "Single-copy transgenic wheat generated through the resolution of complex integration patterns" @default.
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• W2091938644 doi "https://doi.org/10.1073/pnas.96.20.11117" @default.
• W2091938644 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/17996" @default.
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