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• W2091948805 abstract "The investigation of single-neuron parameters is of great interest because many aspects in the behavior and communication of neuronal networks still remain unidentified. However, the present available techniques for single-cell measurements are slow and do not allow for a high-throughput approach. We present here a CMOS compatible microelectrode array with 84 electrodes (with diameters ranging from 1.2 to 4.2 μm) that are smaller than the size of cell, thereby supporting single-cell addressability. We show controllable electroporation of a single cell by an underlying electrode while monitoring changes in the intracellular membrane potential. Further, by applying a localized electrical field between two electrodes close to a neuron while recording changes in the intracellular calcium concentration, we demonstrate activation of a single cell (∼270%, DF/F0), followed by a network response of the neighboring cells. The technology can be easily scaled up to larger electrode arrays (theoretically up to 137,000 electrodes/mm2) with active CMOS electronics integration able to perform high-throughput measurements on single cells." @default.
• W2091948805 created "2016-06-24" @default.
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• W2091948805 date "2010-12-01" @default.
• W2091948805 modified "2023-10-18" @default.
• W2091948805 title "Localized electrical stimulation of in vitro neurons using an array of sub-cellular sized electrodes" @default.
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• W2091948805 doi "https://doi.org/10.1016/j.bios.2010.07.086" @default.
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