FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2091979452> ?p ?o ?g. }

• W2091979452 endingPage "1144" @default.
• W2091979452 startingPage "1142" @default.
• W2091979452 abstract "To the Editor: Cantagalo virus (CTGV) is a strain of vaccinia virus (VACV; Poxviridae) that was isolated from pustular lesions on dairy cattle and dairy workers in Rio de Janeiro State, Brazil, in 1999 (1). Subsequently, similar lesions caused by poxviruses have been reported in cattle and humans in all 4 states of the southeast region of Brazil and in Goias State in central-western Brazil (Technical Appendix, panel A, (2–7). Etiologic agents were VACV strains, most of which were genetically related to CTGV, such as Aracatuba and Passatempo viruses (2,4), with the exception of Guarani P1 virus, which was isolated in Minas Gerais State in 2001 and is phylogenetically close to VACV strain WR (5). Reisolation of Guarani P1 virus has not been reported. All VACV isolates related to CTGV share 2 molecular signatures: an 18-nt deletion in the A56R gene, which encodes viral hemagglutinin (1–7), and a 15-nt deletion in K2L gene, which encodes serine protease inhibitor-3 (8,9). Other VACV strains unrelated to CTGV were isolated from rodents in Brazil before 1999, but reisolation of these viruses has not been described (8). Although CTGV-like disease has not been reported in the northern, northeastern, and southern regions of the country, rapid interstate spread of CTGV infection is of concern. We report an episode of CTGV infection in Tocantins State, northern Brazil (Technical Appendix, panel A).In September 2008, teat and udder lesions were found on 15 of 356 febrile (39.5°C–40°C) cattle on a dairy farm in the municipality of Muricilândia. Small papules progressed to vesicles and pustules (Technical Appendix, panel B), which usually healed in 3–4 weeks. New lesions subsequently appeared on previously healthy cows on the same farm, and muzzle lesions developed on suckling calves. Dairy workers reported fever and lesions on their hands and neck. The farm was quarantined for 3 weeks until the condition was diagnosed.Four scab samples were sent for virus identification by PCR. Parts of the samples were used to infect BSC-40 cells and for DNA isolation by phenol-chloroform extraction, as described (6). After 48 hours, a strong cytopathic effect suggested poxvirus infection. The PCR used unambiguously differentiates CTGV-related infections from other orthopoxvirus diseases, including cowpox virus and several VACV strains (6). The reverse primer targets nucleotide sequences flanking the deletion signature of the hemagglutinin gene from CTGV-related viruses. Therefore, a specific annealing site for the reverse primer is produced when these external sequences are contiguous, as occurs in CTGV (6).The full-length hemagglutinin gene (≈900 bp) was detected in all clinical isolates and in the control DNA samples from CTGV, VACV strain WR, and cowpox virus strain Brighton-red (Technical Appendix, panel C). Nevertheless, when we used the primers specific for CTGV detection, only CTGV and the 4 isolates were positive, generating 714-bp fragments, which indicated CTGV as the etiologic agent. In late November, the disease was reported in 9 cattle in Santa Fe do Araguaia, a municipality 12 km west of Muricilândia. Those samples were also positive for CTGV by PCR (data not shown).For phylogenetic inference, we used DNA from the isolate MU-07 to sequence the genes A56R (927 bp), C7L (453 bp) that encodes a host-range virulence factor, and K2L (1095 bp); primers aligned externally to the open reading frames. PCR and sequencing were performed as described elsewhere (1). Sequences were deposited in GenBank (accession nos. {type:entrez-nucleotide,attrs:{text:FJ545689,term_id:242347664,term_text:FJ545689}}FJ545689, {type:entrez-nucleotide,attrs:{text:FJ545688,term_id:242347662,term_text:FJ545688}}FJ545688, and {type:entrez-nucleotide,attrs:{text:FJ545687,term_id:242347660,term_text:FJ545687}}FJ545687, respectively). Nucleotide identities in relation to CTGV sequences were 99.8% (A56R), 100% (C7L), and 100% (K2L). Both A56R and K2L genes had deletions considered to be molecular signatures for Brazilian VACV related to CTGV. Phylogenetic inference of the concatenated nucleotide dataset of 27 orthopoxviruses shows that the causative agent grouped with other Brazilian VACV related to CTGV (Technical Appendix, panel D).We consider the etiologic agent of the infection in Tocantins State to be a CTGV isolate, indicating spread of CTGV infection to northern Brazil. This spread could reflect interstate propagation of the virus due to movement of animals or people, which is particularly intense at the southern border with Goias State (location of the nearest CTGV outbreak) (3). Nevertheless, no epidemiologic data are available to support a relationship between these episodes. The Agency for Animal Health Defense of Tocantins State has not been previously notified of clinical suspicion of poxvirus infection in dairy herds.Another concern is spread of the virus to water buffalo, which account for a growing farming industry in Brazil, specifically in the northern states (www.ibge.gov.br/english). Infected buffalo have not yet been reported in Brazil, but the establishment of VACV strains in buffaloes has long been reported in India; economic losses have been substantial (9). Therefore, a careful survey should be conducted to evaluate dissemination of the virus to other states and species in the Amazon region." @default.
• W2091979452 created "2016-06-24" @default.
• W2091979452 creator A5012834081 @default.
• W2091979452 creator A5023581603 @default.
• W2091979452 creator A5025402163 @default.
• W2091979452 creator A5073115609 @default.
• W2091979452 creator A5083680078 @default.
• W2091979452 date "2009-07-01" @default.
• W2091979452 modified "2023-09-24" @default.
• W2091979452 title "Spread of Cantagalo Virus to Northern Brazil" @default.
• W2091979452 cites W1860559338 @default.
• W2091979452 cites W1985338141 @default.
• W2091979452 cites W2042700226 @default.
• W2091979452 cites W2073522251 @default.
• W2091979452 cites W2077283859 @default.
• W2091979452 cites W2080039093 @default.
• W2091979452 cites W2153423532 @default.
• W2091979452 cites W2153490477 @default.
• W2091979452 cites W2170866541 @default.
• W2091979452 doi "https://doi.org/10.3201/eid1507.081702" @default.
• W2091979452 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2744230" @default.
• W2091979452 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19624947" @default.
• W2091979452 hasPublicationYear "2009" @default.
• W2091979452 type Work @default.
• W2091979452 sameAs 2091979452 @default.
• W2091979452 citedByCount "40" @default.
• W2091979452 countsByYear W20919794522012 @default.
• W2091979452 countsByYear W20919794522013 @default.
• W2091979452 countsByYear W20919794522014 @default.
• W2091979452 countsByYear W20919794522015 @default.
• W2091979452 countsByYear W20919794522016 @default.
• W2091979452 countsByYear W20919794522017 @default.
• W2091979452 countsByYear W20919794522018 @default.
• W2091979452 countsByYear W20919794522019 @default.
• W2091979452 countsByYear W20919794522020 @default.
• W2091979452 countsByYear W20919794522021 @default.
• W2091979452 countsByYear W20919794522022 @default.
• W2091979452 countsByYear W20919794522023 @default.
• W2091979452 crossrefType "journal-article" @default.
• W2091979452 hasAuthorship W2091979452A5012834081 @default.
• W2091979452 hasAuthorship W2091979452A5023581603 @default.
• W2091979452 hasAuthorship W2091979452A5025402163 @default.
• W2091979452 hasAuthorship W2091979452A5073115609 @default.
• W2091979452 hasAuthorship W2091979452A5083680078 @default.
• W2091979452 hasBestOaLocation W20919794521 @default.
• W2091979452 hasConcept C159047783 @default.
• W2091979452 hasConcept C205649164 @default.
• W2091979452 hasConcept C2522874641 @default.
• W2091979452 hasConcept C86803240 @default.
• W2091979452 hasConceptScore W2091979452C159047783 @default.
• W2091979452 hasConceptScore W2091979452C205649164 @default.
• W2091979452 hasConceptScore W2091979452C2522874641 @default.
• W2091979452 hasConceptScore W2091979452C86803240 @default.
• W2091979452 hasIssue "7" @default.
• W2091979452 hasLocation W20919794521 @default.
• W2091979452 hasLocation W20919794522 @default.
• W2091979452 hasLocation W20919794523 @default.
• W2091979452 hasLocation W20919794524 @default.
• W2091979452 hasLocation W20919794525 @default.
• W2091979452 hasOpenAccess W2091979452 @default.
• W2091979452 hasPrimaryLocation W20919794521 @default.
• W2091979452 hasRelatedWork W2016551366 @default.
• W2091979452 hasRelatedWork W2056772893 @default.
• W2091979452 hasRelatedWork W2121676840 @default.
• W2091979452 hasRelatedWork W2123163123 @default.
• W2091979452 hasRelatedWork W2138564085 @default.
• W2091979452 hasRelatedWork W2142062823 @default.
• W2091979452 hasRelatedWork W2188759708 @default.
• W2091979452 hasRelatedWork W2331480949 @default.
• W2091979452 hasRelatedWork W2400613564 @default.
• W2091979452 hasRelatedWork W2406270448 @default.
• W2091979452 hasVolume "15" @default.
• W2091979452 isParatext "false" @default.
• W2091979452 isRetracted "false" @default.
• W2091979452 magId "2091979452" @default.
• W2091979452 workType "article" @default.