Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092017658> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2092017658 endingPage "381" @default.
- W2092017658 startingPage "371" @default.
- W2092017658 abstract "To transfer genes into airway epithelial cells, we have generated auxotrophic dap Escherichia coli BM2710 mutant that expresses the invasin of Yersinia pseudotuberculosis and the listeriolysin of Listeria monocytogenes. E. coli BM2710 harboring a plasmid carrying the gfp gene was incubated with immortalized normal or cystic fibrosis (CF) airway epithelial cells or with primary bronchial epithelial cells grown as an explant-outgrowth cell culture model. Approximately 2% of immortalized cells expressed GFP. Few primary cells were transfected that were always poorly differentiated and located at the edge of the outgrowth. This was consistent with the expression of beta1-integrins only on these cells and with the required interaction for cell entry of E. coli expressing the invasin with beta1-integrins. The subsequent intracellular trafficking of E. coli BM2710 studied by confocal and electronic microscopy showed that the E. coli-containing phagosomes rapidly matured into phagolysosomes. This is the first demonstration that recombinant bacteria are able to transfer genes into primary airway epithelial cells, provided that they are able to invade the cells." @default.
- W2092017658 created "2016-06-24" @default.
- W2092017658 creator A5013358596 @default.
- W2092017658 creator A5030654528 @default.
- W2092017658 creator A5035108761 @default.
- W2092017658 creator A5042426737 @default.
- W2092017658 creator A5056720235 @default.
- W2092017658 creator A5073987241 @default.
- W2092017658 creator A5078175618 @default.
- W2092017658 date "2004-06-01" @default.
- W2092017658 modified "2023-10-17" @default.
- W2092017658 title "Recombinant Escherichia coli as a gene delivery vector into airway epithelial cells" @default.
- W2092017658 cites W1520821663 @default.
- W2092017658 cites W1966811207 @default.
- W2092017658 cites W1974540875 @default.
- W2092017658 cites W1974541422 @default.
- W2092017658 cites W1981569852 @default.
- W2092017658 cites W1992229324 @default.
- W2092017658 cites W2005644246 @default.
- W2092017658 cites W2016767984 @default.
- W2092017658 cites W2019662066 @default.
- W2092017658 cites W2020250787 @default.
- W2092017658 cites W2039599894 @default.
- W2092017658 cites W2048602230 @default.
- W2092017658 cites W2057625458 @default.
- W2092017658 cites W2065606748 @default.
- W2092017658 cites W2079439306 @default.
- W2092017658 cites W2083337385 @default.
- W2092017658 cites W2166627321 @default.
- W2092017658 cites W2324487154 @default.
- W2092017658 doi "https://doi.org/10.1016/j.jconrel.2004.03.025" @default.
- W2092017658 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15196763" @default.
- W2092017658 hasPublicationYear "2004" @default.
- W2092017658 type Work @default.
- W2092017658 sameAs 2092017658 @default.
- W2092017658 citedByCount "30" @default.
- W2092017658 countsByYear W20920176582012 @default.
- W2092017658 countsByYear W20920176582013 @default.
- W2092017658 countsByYear W20920176582017 @default.
- W2092017658 countsByYear W20920176582018 @default.
- W2092017658 countsByYear W20920176582020 @default.
- W2092017658 countsByYear W20920176582021 @default.
- W2092017658 countsByYear W20920176582023 @default.
- W2092017658 crossrefType "journal-article" @default.
- W2092017658 hasAuthorship W2092017658A5013358596 @default.
- W2092017658 hasAuthorship W2092017658A5030654528 @default.
- W2092017658 hasAuthorship W2092017658A5035108761 @default.
- W2092017658 hasAuthorship W2092017658A5042426737 @default.
- W2092017658 hasAuthorship W2092017658A5056720235 @default.
- W2092017658 hasAuthorship W2092017658A5073987241 @default.
- W2092017658 hasAuthorship W2092017658A5078175618 @default.
- W2092017658 hasConcept C104317684 @default.
- W2092017658 hasConcept C142613039 @default.
- W2092017658 hasConcept C153911025 @default.
- W2092017658 hasConcept C2781350384 @default.
- W2092017658 hasConcept C523546767 @default.
- W2092017658 hasConcept C54009773 @default.
- W2092017658 hasConcept C54355233 @default.
- W2092017658 hasConcept C547475151 @default.
- W2092017658 hasConcept C55493867 @default.
- W2092017658 hasConcept C81885089 @default.
- W2092017658 hasConcept C86803240 @default.
- W2092017658 hasConcept C89423630 @default.
- W2092017658 hasConcept C95444343 @default.
- W2092017658 hasConceptScore W2092017658C104317684 @default.
- W2092017658 hasConceptScore W2092017658C142613039 @default.
- W2092017658 hasConceptScore W2092017658C153911025 @default.
- W2092017658 hasConceptScore W2092017658C2781350384 @default.
- W2092017658 hasConceptScore W2092017658C523546767 @default.
- W2092017658 hasConceptScore W2092017658C54009773 @default.
- W2092017658 hasConceptScore W2092017658C54355233 @default.
- W2092017658 hasConceptScore W2092017658C547475151 @default.
- W2092017658 hasConceptScore W2092017658C55493867 @default.
- W2092017658 hasConceptScore W2092017658C81885089 @default.
- W2092017658 hasConceptScore W2092017658C86803240 @default.
- W2092017658 hasConceptScore W2092017658C89423630 @default.
- W2092017658 hasConceptScore W2092017658C95444343 @default.
- W2092017658 hasIssue "2" @default.
- W2092017658 hasLocation W20920176581 @default.
- W2092017658 hasLocation W20920176582 @default.
- W2092017658 hasOpenAccess W2092017658 @default.
- W2092017658 hasPrimaryLocation W20920176581 @default.
- W2092017658 hasRelatedWork W119215873 @default.
- W2092017658 hasRelatedWork W1491710477 @default.
- W2092017658 hasRelatedWork W1599991822 @default.
- W2092017658 hasRelatedWork W2285828597 @default.
- W2092017658 hasRelatedWork W2377277576 @default.
- W2092017658 hasRelatedWork W2469005932 @default.
- W2092017658 hasRelatedWork W2496396261 @default.
- W2092017658 hasRelatedWork W2622810228 @default.
- W2092017658 hasRelatedWork W2730826456 @default.
- W2092017658 hasRelatedWork W2963664721 @default.
- W2092017658 hasVolume "97" @default.
- W2092017658 isParatext "false" @default.
- W2092017658 isRetracted "false" @default.
- W2092017658 magId "2092017658" @default.
- W2092017658 workType "article" @default.