FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092018737> ?p ?o ?g. }

• W2092018737 endingPage "5067" @default.
• W2092018737 startingPage "5058" @default.
• W2092018737 abstract "The TSH receptor (TSHR) hinge region, the least well understood component, bridges the leucine-rich repeat and transmembrane domains. We report data on clusters of hinge charged residues the mutation of which to Ala is compatible with cell surface expression and normal, or near normal, TSH binding affinity yet with a relative reduction in receptor activation. Mutation to Ala of E409 at the junction with the transmembrane domain was the most potent in uncoupling TSH binding and signal transduction (~22-fold less sensitive than the wild-type TSHR) and was unique among the residues studied in reducing both the amplitude and the sensitivity of the ligand-induced signal. Unexpectedly, a dual E409A/D410A mutation partially corrected the major suppressive effect of TSHR-E409A. The combined Ala substitution of a cluster of positively charged hinge residues (K287, K290, K291, R293; termed K3R1) synergistically reduced sensitivity to TSH stimulation approximately 21-fold without altering the TSH binding affinity. Simultaneous Ala substitutions of a cluster of acidic hinge residues D392, E394, and D395 (termed DE392-5A) partially uncoupled TSH binding from signal transduction (4.4-fold reduction in sensitivity), less than for E409A and K3R1A. Remarkably, the combination of the K3R1A and DE392-5A mutations was not additive but ameliorated the major uncoupling effect of K3R1A. This lack of additivity suggests that these two clusters contribute to a common signaling pathway. In summary, we identify several TSHR hinge residues involved in signal transmission. Our data support the concept that the hinge regions of the TSHR (and other glycoprotein hormone receptors) act as surrogate ligands for receptor activation." @default.
• W2092018737 created "2016-06-24" @default.
• W2092018737 creator A5018820715 @default.
• W2092018737 creator A5022600211 @default.
• W2092018737 creator A5044929199 @default.
• W2092018737 creator A5055826150 @default.
• W2092018737 date "2012-10-01" @default.
• W2092018737 modified "2023-09-25" @default.
• W2092018737 title "The Thyrotropin Receptor Hinge Region as a Surrogate Ligand: Identification of Loci Contributing to the Coupling of Thyrotropin Binding and Receptor Activation" @default.
• W2092018737 cites W1505110999 @default.
• W2092018737 cites W1512361626 @default.
• W2092018737 cites W1825353903 @default.
• W2092018737 cites W1970749700 @default.
• W2092018737 cites W1971032649 @default.
• W2092018737 cites W1975740938 @default.
• W2092018737 cites W1980962873 @default.
• W2092018737 cites W1981685546 @default.
• W2092018737 cites W1986447682 @default.
• W2092018737 cites W1989226450 @default.
• W2092018737 cites W1995900037 @default.
• W2092018737 cites W1998387943 @default.
• W2092018737 cites W2007702516 @default.
• W2092018737 cites W2012527285 @default.
• W2092018737 cites W2012681252 @default.
• W2092018737 cites W2019972073 @default.
• W2092018737 cites W2021759177 @default.
• W2092018737 cites W2031854799 @default.
• W2092018737 cites W2037941418 @default.
• W2092018737 cites W2047924793 @default.
• W2092018737 cites W2062007063 @default.
• W2092018737 cites W2062754157 @default.
• W2092018737 cites W2069320780 @default.
• W2092018737 cites W2070413673 @default.
• W2092018737 cites W2078190500 @default.
• W2092018737 cites W2078357110 @default.
• W2092018737 cites W2079739758 @default.
• W2092018737 cites W2091089626 @default.
• W2092018737 cites W2092202249 @default.
• W2092018737 cites W2096929449 @default.
• W2092018737 cites W2100479663 @default.
• W2092018737 cites W2104477830 @default.
• W2092018737 cites W2104961861 @default.
• W2092018737 cites W2108844645 @default.
• W2092018737 cites W2115185128 @default.
• W2092018737 cites W2120205802 @default.
• W2092018737 cites W2122967721 @default.
• W2092018737 cites W2140740343 @default.
• W2092018737 cites W2142365569 @default.
• W2092018737 cites W2149963584 @default.
• W2092018737 cites W2152853006 @default.
• W2092018737 cites W2167857702 @default.
• W2092018737 cites W4248519364 @default.
• W2092018737 cites W4249432920 @default.
• W2092018737 doi "https://doi.org/10.1210/en.2012-1376" @default.
• W2092018737 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3512008" @default.
• W2092018737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23002040" @default.
• W2092018737 hasPublicationYear "2012" @default.
• W2092018737 type Work @default.
• W2092018737 sameAs 2092018737 @default.
• W2092018737 citedByCount "9" @default.
• W2092018737 countsByYear W20920187372012 @default.
• W2092018737 countsByYear W20920187372013 @default.
• W2092018737 countsByYear W20920187372015 @default.
• W2092018737 countsByYear W20920187372017 @default.
• W2092018737 countsByYear W20920187372018 @default.
• W2092018737 countsByYear W20920187372020 @default.
• W2092018737 countsByYear W20920187372023 @default.
• W2092018737 crossrefType "journal-article" @default.
• W2092018737 hasAuthorship W2092018737A5018820715 @default.
• W2092018737 hasAuthorship W2092018737A5022600211 @default.
• W2092018737 hasAuthorship W2092018737A5044929199 @default.
• W2092018737 hasAuthorship W2092018737A5055826150 @default.
• W2092018737 hasBestOaLocation W20920187371 @default.
• W2092018737 hasConcept C104317684 @default.
• W2092018737 hasConcept C116569031 @default.
• W2092018737 hasConcept C118892022 @default.
• W2092018737 hasConcept C12554922 @default.
• W2092018737 hasConcept C126322002 @default.
• W2092018737 hasConcept C134018914 @default.
• W2092018737 hasConcept C143065580 @default.
• W2092018737 hasConcept C170493617 @default.
• W2092018737 hasConcept C185592680 @default.
• W2092018737 hasConcept C2776652619 @default.
• W2092018737 hasConcept C2777608072 @default.
• W2092018737 hasConcept C501734568 @default.
• W2092018737 hasConcept C526584372 @default.
• W2092018737 hasConcept C55493867 @default.
• W2092018737 hasConcept C62478195 @default.
• W2092018737 hasConcept C71924100 @default.
• W2092018737 hasConcept C86803240 @default.
• W2092018737 hasConceptScore W2092018737C104317684 @default.
• W2092018737 hasConceptScore W2092018737C116569031 @default.
• W2092018737 hasConceptScore W2092018737C118892022 @default.
• W2092018737 hasConceptScore W2092018737C12554922 @default.
• W2092018737 hasConceptScore W2092018737C126322002 @default.
• W2092018737 hasConceptScore W2092018737C134018914 @default.
• W2092018737 hasConceptScore W2092018737C143065580 @default.
• W2092018737 hasConceptScore W2092018737C170493617 @default.

• next