FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092028051> ?p ?o ?g. }

• W2092028051 endingPage "197" @default.
• W2092028051 startingPage "192" @default.
• W2092028051 abstract "We describe a simple and expedited microneutralization assay for human metapneumovirus virus (HMPV) based on a recombinant HMPV expressing the enhanced green fluorescent protein (rHMPV-GFP). Test serum dilutions were incubated with fixed amounts of rHMPV-GFP and inoculated onto Vero cells, and the growth of non-neutralized rHMPV-GFP was visualized by fluorescent microscopy of living cells. A preliminary titer could be determined following 3 days of incubation. GFP expression was sufficient to be read by an automated scanner after 4–5 days of incubation, which also provided a permanent record. In comparison, the conventional serum neutralization assay requires a longer incubation time plus the additional steps of fixation and staining or immunostaining. rHMPV-GFP-based titers could be determined by the 50% infectivity endpoint method of Reed and Muench [Reed, L.J., Muench, H., 1938. A simple method of estimating fifty per cent endpoint. Am. J. Hyg. 27, 493–497], or by automated scanning and non-linear regression to determine the 50% endpoint of GFP fluorescence. The latter method was two- to three-fold more sensitive. This assay also permits automation and up-scaling, making it suitable for broad HMPV seroepidemiology studies and experiments that require large scale serology, such as vaccine studies." @default.
• W2092028051 created "2016-06-24" @default.
• W2092028051 creator A5020113753 @default.
• W2092028051 creator A5027582911 @default.
• W2092028051 creator A5034229509 @default.
• W2092028051 creator A5054805608 @default.
• W2092028051 creator A5057228985 @default.
• W2092028051 creator A5062733502 @default.
• W2092028051 date "2005-09-01" @default.
• W2092028051 modified "2023-10-17" @default.
• W2092028051 title "Rapid human metapneumovirus microneutralization assay based on green fluorescent protein expression" @default.
• W2092028051 cites W1676502913 @default.
• W2092028051 cites W1969779507 @default.
• W2092028051 cites W1973211512 @default.
• W2092028051 cites W1980812064 @default.
• W2092028051 cites W2014258559 @default.
• W2092028051 cites W2024563102 @default.
• W2092028051 cites W2029394530 @default.
• W2092028051 cites W2043370923 @default.
• W2092028051 cites W2057992117 @default.
• W2092028051 cites W2070086650 @default.
• W2092028051 cites W2071301221 @default.
• W2092028051 cites W2127802424 @default.
• W2092028051 cites W2142792374 @default.
• W2092028051 cites W2143018999 @default.
• W2092028051 cites W2149337298 @default.
• W2092028051 cites W2155091877 @default.
• W2092028051 cites W2170380549 @default.
• W2092028051 cites W2170881661 @default.
• W2092028051 doi "https://doi.org/10.1016/j.jviromet.2005.05.005" @default.
• W2092028051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15955576" @default.
• W2092028051 hasPublicationYear "2005" @default.
• W2092028051 type Work @default.
• W2092028051 sameAs 2092028051 @default.
• W2092028051 citedByCount "77" @default.
• W2092028051 countsByYear W20920280512012 @default.
• W2092028051 countsByYear W20920280512013 @default.
• W2092028051 countsByYear W20920280512014 @default.
• W2092028051 countsByYear W20920280512015 @default.
• W2092028051 countsByYear W20920280512016 @default.
• W2092028051 countsByYear W20920280512017 @default.
• W2092028051 countsByYear W20920280512018 @default.
• W2092028051 countsByYear W20920280512019 @default.
• W2092028051 countsByYear W20920280512020 @default.
• W2092028051 countsByYear W20920280512021 @default.
• W2092028051 countsByYear W20920280512022 @default.
• W2092028051 countsByYear W20920280512023 @default.
• W2092028051 crossrefType "journal-article" @default.
• W2092028051 hasAuthorship W2092028051A5020113753 @default.
• W2092028051 hasAuthorship W2092028051A5027582911 @default.
• W2092028051 hasAuthorship W2092028051A5034229509 @default.
• W2092028051 hasAuthorship W2092028051A5054805608 @default.
• W2092028051 hasAuthorship W2092028051A5057228985 @default.
• W2092028051 hasAuthorship W2092028051A5062733502 @default.
• W2092028051 hasConcept C104317684 @default.
• W2092028051 hasConcept C105702510 @default.
• W2092028051 hasConcept C106358424 @default.
• W2092028051 hasConcept C142613039 @default.
• W2092028051 hasConcept C142724271 @default.
• W2092028051 hasConcept C143701427 @default.
• W2092028051 hasConcept C153911025 @default.
• W2092028051 hasConcept C159047783 @default.
• W2092028051 hasConcept C184112429 @default.
• W2092028051 hasConcept C203014093 @default.
• W2092028051 hasConcept C204232928 @default.
• W2092028051 hasConcept C204787440 @default.
• W2092028051 hasConcept C2522874641 @default.
• W2092028051 hasConcept C25642318 @default.
• W2092028051 hasConcept C2776012195 @default.
• W2092028051 hasConcept C2780475896 @default.
• W2092028051 hasConcept C32611913 @default.
• W2092028051 hasConcept C40767141 @default.
• W2092028051 hasConcept C4224716 @default.
• W2092028051 hasConcept C534529494 @default.
• W2092028051 hasConcept C55493867 @default.
• W2092028051 hasConcept C71924100 @default.
• W2092028051 hasConcept C86803240 @default.
• W2092028051 hasConcept C89138240 @default.
• W2092028051 hasConceptScore W2092028051C104317684 @default.
• W2092028051 hasConceptScore W2092028051C105702510 @default.
• W2092028051 hasConceptScore W2092028051C106358424 @default.
• W2092028051 hasConceptScore W2092028051C142613039 @default.
• W2092028051 hasConceptScore W2092028051C142724271 @default.
• W2092028051 hasConceptScore W2092028051C143701427 @default.
• W2092028051 hasConceptScore W2092028051C153911025 @default.
• W2092028051 hasConceptScore W2092028051C159047783 @default.
• W2092028051 hasConceptScore W2092028051C184112429 @default.
• W2092028051 hasConceptScore W2092028051C203014093 @default.
• W2092028051 hasConceptScore W2092028051C204232928 @default.
• W2092028051 hasConceptScore W2092028051C204787440 @default.
• W2092028051 hasConceptScore W2092028051C2522874641 @default.
• W2092028051 hasConceptScore W2092028051C25642318 @default.
• W2092028051 hasConceptScore W2092028051C2776012195 @default.
• W2092028051 hasConceptScore W2092028051C2780475896 @default.
• W2092028051 hasConceptScore W2092028051C32611913 @default.
• W2092028051 hasConceptScore W2092028051C40767141 @default.
• W2092028051 hasConceptScore W2092028051C4224716 @default.
• W2092028051 hasConceptScore W2092028051C534529494 @default.

• next