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- W2092034021 abstract "Usingpara-nitrophenyl phosphate (pNPP) as a substrate for enzymatic activity, we sought to identify CaN inParamecium.We isolated three differentpNPP-phosphatases from the soluble fraction ofParameciumcells by anion-exchange and affinity column chromatographies. One,pNPP-phosphatasePeak I,is very similar to mammalian CaN. Divalent cation dependency, inhibition by calmodulin (CaM) antagonists (trifluoperazine, calmidazolium), and insensitivity to various phosphatase inhibitors (heparin, okadaic acid, sodium vanadate, etc.) show similarity to mammalian CaN rather than to any otherParamecium pNPP-hydrolyzing enzymes tested. Polyclonal antibodies against bovine brain CaN recognizing subunits A (61 or 58 kDa) and B (17 kDa) of brain CaN cross-reacted with a 63-kDa protein in fractions containingPeak I pNPP-phosphatase activity and coeluted calmodulin. Overlay assays using biotinylated brain calmodulin indicated Ca2+-dependent CaM-binding by the 63-kDa protein. A Ca2+-binding protein with the same electrophoretic mobility as CaN B (17 kDa) was also present, though in other fractions from DEAE–cellulose chromatography. This finding strongly suggests that, in the absence of Ca2+, both subunits, A and B, were separated either before or during chromatographic processing. Our data support the existence of both subunits of a CaN-like phosphatase inParameciumcells." @default.
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- W2092034021 date "1997-08-01" @default.
- W2092034021 modified "2023-09-25" @default.
- W2092034021 title "Occurrence of apara-Nitrophenyl Phosphate-Phosphatase with Calcineurin-like Characteristics inParamecium tetraurelia" @default.
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- W2092034021 doi "https://doi.org/10.1006/abbi.1997.0208" @default.
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