FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092080320> ?p ?o ?g. }

• W2092080320 endingPage "29" @default.
• W2092080320 startingPage "1" @default.
• W2092080320 abstract "This paper reports a new technique for reducing resistance to stagnant mobile phase mass transfer without sacrificing high adsorbent capacity or necessitating extremely high pressure operation. The technique involves the flow of liquid through a porous chromatographic particle, and has thus been termed perfusion chromatography. This is accomplished with 6000-8000 A pores which transect the particle. Data from electron microscopy, column efficiency, frontal analysis and theoretical modelling all suggest that mobile phase will flow through these large pores. In this manner, solutes enter the interior of the particles through a combination of convective and diffusional transport, with convection dominating for Peclet numbers greater than one. The implications of flow through particles on bandspreading, resolution and dynamic loading capacity are examined. It is shown that the rate of solute transport is strongly coupled to mobile phase velocity such that bandspreading, resolution of proteins and dynamic loading capacity are unaffected by increases in mobile phase velocity up to several thousand centimeters per hour. The surface area of this very large-pore diameter material is enhanced by using a network of smaller, 500-1500 A interconnecting pores between the throughpores. Scanning electron micrographs show that the pore network is continuous and that no point in the matrix is more than 5000-10,000 A from a through-pore. As a consequence, diffusional path lengths are minimized and the large porous particles take on the transport characteristics of much smaller particles but with a fraction of the pressure drop. Capacity and resolution studies show that these materials bind and separate an amount of protein equivalent to that of conventional high-performance liquid chromatography as well as low performance agarose-based media at greater than 10-100 times higher mobile phase velocity with no loss in resolution." @default.
• W2092080320 created "2016-06-24" @default.
• W2092080320 creator A5014043715 @default.
• W2092080320 creator A5020309429 @default.
• W2092080320 creator A5022438900 @default.
• W2092080320 creator A5075538307 @default.
• W2092080320 creator A5078222979 @default.
• W2092080320 creator A5081563992 @default.
• W2092080320 creator A5082362552 @default.
• W2092080320 date "1990-10-01" @default.
• W2092080320 modified "2023-10-18" @default.
• W2092080320 title "Flow-through particles for the high-performance liquid chromatographic separation of biomolecules: perfusion chromatography" @default.
• W2092080320 cites W1487260360 @default.
• W2092080320 cites W1522451648 @default.
• W2092080320 cites W1582613326 @default.
• W2092080320 cites W1965103059 @default.
• W2092080320 cites W1982416046 @default.
• W2092080320 cites W1991724766 @default.
• W2092080320 cites W1993048802 @default.
• W2092080320 cites W2013664498 @default.
• W2092080320 cites W2016256960 @default.
• W2092080320 cites W2030088577 @default.
• W2092080320 cites W2030163787 @default.
• W2092080320 cites W2040152738 @default.
• W2092080320 cites W2064680209 @default.
• W2092080320 cites W2072432503 @default.
• W2092080320 cites W2080099496 @default.
• W2092080320 cites W2087564501 @default.
• W2092080320 cites W2088411468 @default.
• W2092080320 cites W2091549903 @default.
• W2092080320 cites W2128478738 @default.
• W2092080320 cites W2203017712 @default.
• W2092080320 cites W2321537879 @default.
• W2092080320 cites W4238580586 @default.
• W2092080320 cites W4251632537 @default.
• W2092080320 cites W1981756423 @default.
• W2092080320 doi "https://doi.org/10.1016/0021-9673(90)85132-f" @default.
• W2092080320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2077042" @default.
• W2092080320 hasPublicationYear "1990" @default.
• W2092080320 type Work @default.
• W2092080320 sameAs 2092080320 @default.
• W2092080320 citedByCount "498" @default.
• W2092080320 countsByYear W20920803202012 @default.
• W2092080320 countsByYear W20920803202013 @default.
• W2092080320 countsByYear W20920803202014 @default.
• W2092080320 countsByYear W20920803202015 @default.
• W2092080320 countsByYear W20920803202016 @default.
• W2092080320 countsByYear W20920803202017 @default.
• W2092080320 countsByYear W20920803202018 @default.
• W2092080320 countsByYear W20920803202019 @default.
• W2092080320 countsByYear W20920803202020 @default.
• W2092080320 countsByYear W20920803202021 @default.
• W2092080320 countsByYear W20920803202022 @default.
• W2092080320 countsByYear W20920803202023 @default.
• W2092080320 crossrefType "journal-article" @default.
• W2092080320 hasAuthorship W2092080320A5014043715 @default.
• W2092080320 hasAuthorship W2092080320A5020309429 @default.
• W2092080320 hasAuthorship W2092080320A5022438900 @default.
• W2092080320 hasAuthorship W2092080320A5075538307 @default.
• W2092080320 hasAuthorship W2092080320A5078222979 @default.
• W2092080320 hasAuthorship W2092080320A5081563992 @default.
• W2092080320 hasAuthorship W2092080320A5082362552 @default.
• W2092080320 hasConcept C111368507 @default.
• W2092080320 hasConcept C113196181 @default.
• W2092080320 hasConcept C114088122 @default.
• W2092080320 hasConcept C121332964 @default.
• W2092080320 hasConcept C127313418 @default.
• W2092080320 hasConcept C127413603 @default.
• W2092080320 hasConcept C138268822 @default.
• W2092080320 hasConcept C147789679 @default.
• W2092080320 hasConcept C154945302 @default.
• W2092080320 hasConcept C159985019 @default.
• W2092080320 hasConcept C172120300 @default.
• W2092080320 hasConcept C178790620 @default.
• W2092080320 hasConcept C185592680 @default.
• W2092080320 hasConcept C187530423 @default.
• W2092080320 hasConcept C192562407 @default.
• W2092080320 hasConcept C26771246 @default.
• W2092080320 hasConcept C2778517922 @default.
• W2092080320 hasConcept C41008148 @default.
• W2092080320 hasConcept C42360764 @default.
• W2092080320 hasConcept C43617362 @default.
• W2092080320 hasConcept C44280652 @default.
• W2092080320 hasConcept C51038369 @default.
• W2092080320 hasConcept C57879066 @default.
• W2092080320 hasConcept C6648577 @default.
• W2092080320 hasConceptScore W2092080320C111368507 @default.
• W2092080320 hasConceptScore W2092080320C113196181 @default.
• W2092080320 hasConceptScore W2092080320C114088122 @default.
• W2092080320 hasConceptScore W2092080320C121332964 @default.
• W2092080320 hasConceptScore W2092080320C127313418 @default.
• W2092080320 hasConceptScore W2092080320C127413603 @default.
• W2092080320 hasConceptScore W2092080320C138268822 @default.
• W2092080320 hasConceptScore W2092080320C147789679 @default.
• W2092080320 hasConceptScore W2092080320C154945302 @default.
• W2092080320 hasConceptScore W2092080320C159985019 @default.
• W2092080320 hasConceptScore W2092080320C172120300 @default.
• W2092080320 hasConceptScore W2092080320C178790620 @default.

• next