FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092108820> ?p ?o ?g. }

• W2092108820 endingPage "3328" @default.
• W2092108820 startingPage "3319" @default.
• W2092108820 abstract "The stability of nonviral vectors during freeze-drying has been well-studied, and it has been established that sugars can protect lipoplexes during freeze-drying. However low levels of damage are often observed after freeze-drying, and this damage is more evident in dilute lipoplex preparations. By investigating the stability of lipoplexes after each step in the freeze-drying cycle (i.e., freezing, primary drying, and secondary drying), we strive to understand the mechanisms responsible for damage and identify improved stabilization strategies. N-(1-(2,3-dioleoyloxy)propyl)-N,N,N-trimethylammonium chloride (DOTAP)–cholesterol/plasmid DNA lipoplexes were prepared at an equimolar DOTAP–cholesterol ratio, and a 3:1 DOTAP+–DNA− charge ratio. Our experiments indicate that despite sufficient levels of “stabilizing” sugars, significant damage is still evident when dilute lipoplex preparations are subjected to freeze-drying. Analysis of the different stages of freeze-drying suggests that significant damage occurs during freezing, and that sugars have a limited capacity to protect against this freezing-induced damage. Similar effects have been observed in studies with proteins, and surfactants have been employed in protein formulations to protect against surface-induced damage, for example, at the ice crystal, solid, air, or sugar glass surfaces. However, the use of surfactants in a lipid-based formulation is inherently risky due to the potential for altering/solubilizing the lipid delivery vehicle. Our data indicate that judicious use of surfactants can reduce surface-induced damage and result in better preservation of lipoplex size and transfection activity after freeze-drying. © 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 98:3319–3328, 2009" @default.
• W2092108820 created "2016-06-24" @default.
• W2092108820 creator A5017340976 @default.
• W2092108820 creator A5036952731 @default.
• W2092108820 date "2009-09-01" @default.
• W2092108820 modified "2023-09-25" @default.
• W2092108820 title "Synergistic effects of surfactants and sugars on lipoplex stability during freeze-drying and rehydration" @default.
• W2092108820 cites W1603523234 @default.
• W2092108820 cites W1988089792 @default.
• W2092108820 cites W1992736089 @default.
• W2092108820 cites W2002975534 @default.
• W2092108820 cites W2006231275 @default.
• W2092108820 cites W2007897007 @default.
• W2092108820 cites W2011989381 @default.
• W2092108820 cites W2018310031 @default.
• W2092108820 cites W2018892922 @default.
• W2092108820 cites W2024186992 @default.
• W2092108820 cites W2031189480 @default.
• W2092108820 cites W2037385269 @default.
• W2092108820 cites W2039683568 @default.
• W2092108820 cites W2066662804 @default.
• W2092108820 cites W2068931116 @default.
• W2092108820 cites W2074762435 @default.
• W2092108820 cites W2075171273 @default.
• W2092108820 cites W2077171402 @default.
• W2092108820 cites W2084064231 @default.
• W2092108820 cites W20847613 @default.
• W2092108820 cites W2086877095 @default.
• W2092108820 cites W2086919915 @default.
• W2092108820 cites W2091444231 @default.
• W2092108820 cites W2095151181 @default.
• W2092108820 cites W2124018243 @default.
• W2092108820 cites W2131461139 @default.
• W2092108820 cites W2132678460 @default.
• W2092108820 cites W2142987628 @default.
• W2092108820 cites W2164202115 @default.
• W2092108820 cites W224574185 @default.
• W2092108820 cites W2572979827 @default.
• W2092108820 cites W4240268819 @default.
• W2092108820 cites W48033824 @default.
• W2092108820 doi "https://doi.org/10.1002/jps.21564" @default.
• W2092108820 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2721014" @default.
• W2092108820 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18781626" @default.
• W2092108820 hasPublicationYear "2009" @default.
• W2092108820 type Work @default.
• W2092108820 sameAs 2092108820 @default.
• W2092108820 citedByCount "23" @default.
• W2092108820 countsByYear W20921088202012 @default.
• W2092108820 countsByYear W20921088202013 @default.
• W2092108820 countsByYear W20921088202015 @default.
• W2092108820 countsByYear W20921088202017 @default.
• W2092108820 countsByYear W20921088202018 @default.
• W2092108820 countsByYear W20921088202019 @default.
• W2092108820 countsByYear W20921088202020 @default.
• W2092108820 countsByYear W20921088202021 @default.
• W2092108820 crossrefType "journal-article" @default.
• W2092108820 hasAuthorship W2092108820A5017340976 @default.
• W2092108820 hasAuthorship W2092108820A5036952731 @default.
• W2092108820 hasBestOaLocation W20921088202 @default.
• W2092108820 hasConcept C127413603 @default.
• W2092108820 hasConcept C143425029 @default.
• W2092108820 hasConcept C185592680 @default.
• W2092108820 hasConcept C2777108408 @default.
• W2092108820 hasConcept C31903555 @default.
• W2092108820 hasConcept C42360764 @default.
• W2092108820 hasConcept C43617362 @default.
• W2092108820 hasConcept C552990157 @default.
• W2092108820 hasConcept C55493867 @default.
• W2092108820 hasConcept C84199377 @default.
• W2092108820 hasConceptScore W2092108820C127413603 @default.
• W2092108820 hasConceptScore W2092108820C143425029 @default.
• W2092108820 hasConceptScore W2092108820C185592680 @default.
• W2092108820 hasConceptScore W2092108820C2777108408 @default.
• W2092108820 hasConceptScore W2092108820C31903555 @default.
• W2092108820 hasConceptScore W2092108820C42360764 @default.
• W2092108820 hasConceptScore W2092108820C43617362 @default.
• W2092108820 hasConceptScore W2092108820C552990157 @default.
• W2092108820 hasConceptScore W2092108820C55493867 @default.
• W2092108820 hasConceptScore W2092108820C84199377 @default.
• W2092108820 hasIssue "9" @default.
• W2092108820 hasLocation W20921088201 @default.
• W2092108820 hasLocation W20921088202 @default.
• W2092108820 hasLocation W20921088203 @default.
• W2092108820 hasLocation W20921088204 @default.
• W2092108820 hasOpenAccess W2092108820 @default.
• W2092108820 hasPrimaryLocation W20921088201 @default.
• W2092108820 hasRelatedWork W2023462805 @default.
• W2092108820 hasRelatedWork W2033517638 @default.
• W2092108820 hasRelatedWork W2349583355 @default.
• W2092108820 hasRelatedWork W2748952813 @default.
• W2092108820 hasRelatedWork W3033815264 @default.
• W2092108820 hasRelatedWork W3044812762 @default.
• W2092108820 hasRelatedWork W3088489642 @default.
• W2092108820 hasRelatedWork W3143852607 @default.
• W2092108820 hasRelatedWork W3196280815 @default.
• W2092108820 hasRelatedWork W4236524246 @default.
• W2092108820 hasVolume "98" @default.
• W2092108820 isParatext "false" @default.

• next