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- W2092127837 abstract "Bovine herpesvirus-1 (BHV-1) glycoprotein D (gD), an envelope glycoprotein, engenders mucosal and systemic immunity protecting cattle from viral infection. Production of gD with authentic immunogenicity is required for a subunit vaccine. We placed the truncated BHV-1 gD gene, lacking its putative transmembrane and cytoplasmic domains, under the control of the methanol-inducible AOX1 promoter in the yeast Pichia pastoris. Truncated BHV-1 gD (tgD) was efficiently secreted into the culture medium as a 68 kDa protein using either the yeast alpha prepro or native BHV-1 gD signal sequences. The yeast-secreted tgD had N-linked glycosylation and appears to have authentic conformational structure and immunogenicity based on the following observations. A panel of monoclonal antibodies recognizing five neutralizing epitopes reacted with yeast tgD. Sera from yeast tgD-immunized mice immunoprecipitated native BHV-1 gD and neutralized BHV-1 infection in vitro. Yeast tgD competitively blocked all reaction between native gD and monospecific gD polyclonal sera from cattle. Based on these data, yeast-derived BHV-1 tgD is an excellent candidate for a subunit vaccine." @default.
- W2092127837 created "2016-06-24" @default.
- W2092127837 creator A5068183457 @default.
- W2092127837 creator A5068299250 @default.
- W2092127837 creator A5072737251 @default.
- W2092127837 date "1997-04-01" @default.
- W2092127837 modified "2023-09-26" @default.
- W2092127837 title "Yeast-secreted bovine herpesvirus type 1 glycoprotein D has authentic conformational structure and immunogenicity" @default.
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- W2092127837 doi "https://doi.org/10.1016/s0264-410x(96)00234-4" @default.
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