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• W2092136345 abstract "Article6 October 2005free access Phosphorylation of ephrin-B1 via the interaction with claudin following cell–cell contact formation Masamitsu Tanaka Masamitsu Tanaka Growth Factor Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan Search for more papers by this author Reiko Kamata Reiko Kamata Growth Factor Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan Search for more papers by this author Ryuichi Sakai Corresponding Author Ryuichi Sakai Growth Factor Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan Search for more papers by this author Masamitsu Tanaka Masamitsu Tanaka Growth Factor Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan Search for more papers by this author Reiko Kamata Reiko Kamata Growth Factor Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan Search for more papers by this author Ryuichi Sakai Corresponding Author Ryuichi Sakai Growth Factor Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan Search for more papers by this author Author Information Masamitsu Tanaka1, Reiko Kamata1 and Ryuichi Sakai 1 1Growth Factor Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan *Corresponding author. Growth Factor Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan. Tel.: +81 3 3542 2511; Fax: +81 3 3542 8170; E-mail: [email protected] The EMBO Journal (2005)24:3700-3711https://doi.org/10.1038/sj.emboj.7600831 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The interaction of the Eph family of receptor protein tyrosine kinase and its ligand ephrin family induces bidirectional signaling via the cell–cell contacts. Although most previous studies have focused on the function of Eph–ephrin pathways in the neural system and endothelial cells, this process also occurs in epithelial and cancer cells, of which the biological involvement is poorly understood. We show that ephrin-B1 creates an in vivo complex with adjacent claudin1 or claudin4 via the extracellular domains of these proteins. The cytoplasmic domain of ephrin-B1 was phosphorylated on tyrosine residues upon the formation of cell–cell contacts, possibly recognizing an intercellular adhesion of claudins. Phosphorylation of ephrin-B1 induced by claudins was abolished by the treatment with 4-amino-5-(4-chlorophenyl)-7–(t-butyl)pyrazolo[3,4-d]pyrimidine, an inhibitor of the Src family kinases. Moreover, overexpression of ephrin-B1 triggered consequent change in the level of cell–cell adhesion depending on its phosphorylation. These results suggest that ephrin-B1 mediated the cell–cell adhesion of epithelial and cancer cells via a novel Eph receptor-independent mechanism. Introduction The members of the Eph receptor family can be classified into two groups based on their sequence similarity and preferential binding to a subset of ligands tethered to the cell surface either by a glycosylphosphatidyl inositol anchor (ephrin-A) or a transmembrane domain (ephrin-B). The transmembrane ligand ephrin-B1 has been shown to be phosphorylated on tyrosine residues following contact with the corresponding Eph receptor ectodomain, and to have a receptor-like intrinsic signaling potential leading to transduce reverse signaling (Holland et al, 1996; Bruckner et al, 1997). At present, two major mechanisms are known to transduce signaling from ephrin-B1 (Kullander and Klein, 2002; Murai and Pasquale, 2003; Poliakov et al, 2004). The receptor-induced tyrosine phosphorylation of ephrin-B1 recruits Grb4 SH-adaptor protein. Since Grb4 has three SH3 domains, it could link ephrin-B1 to a vast array of signaling molecules (Cowan and Henkemeyer, 2001). Ephrin-B1 also contains PDZ-binding motif at the carboxyl-terminus, which mediates signals by association with proteins, including some phosphatase and GTPase-activating protein of heterotrimeric G proteins (Torres et al, 1998; Lin et al, 1999; Lu et al, 2001; Palmer et al, 2002). Although Eph receptors and ephrins play essential roles for proper axon pathfinding in the nervous system, some members of the Eph receptors and their ligands are expressed in epithelial cells. For example, EphB2, EphB3 and ephrin-B1 are reciprocally expressed along the crypt–villus axis in the small intestine, which controls the cell positioning along the crypt axis (Batlle et al, 2002). Moreover, the overexpression of Ephs and ephrins is reported in various tumors of epithelial origin (Brantley-Sieders et al, 2004; Surawska et al, 2004). However, the biological significance of Eph/ephrin in epithelial cells or carcinomas is still poorly understood. When ephrin-B1 is overexpressed in the embryos of Xenopus laevis at an early developmental stage, the cell–cell adhesion of the blastomeres was significantly reduced, leading to fatal effects for the embryos (Jones et al, 1998). However, precise identification of the adhesion molecules involved in this phenomenon is still not clear. In order to examine whether ephrin-B1 mediates cell–cell adhesion in the mammalian epithelial cells as well, we established an MDCK cell line, showing the inducible expression of ephrin-B1 through the addition of doxycycline. The MDCK cells expressing ephrin-B1 at a high level tend to loosen the cell–cell adhesion when the cells are compared without expressing ephrin-B1. These observations led us to focus on assessing whether ephrin-B1 could be a mediator of cell–cell adhesion in epithelial cells. While analyzing the proteins involved in the complex formation with ephrin-B1, we identified claudins, which are the major constituents of the tight junctions of epithelial cells. Tight junctions locate in the most apical parts of the lateral membrane and serve as paracellular barriers to restrict the movement of ions and proteins across cell boundaries. Claudins are tetraspan transmembrane proteins comprising a multi-gene family with more than 20 members, and creating a complex with ZO-1, ZO-2 and ZO-3, which represent plaque structures underlying plasma membranes (Morita et al, 1999; Tsukita et al, 2001). Moreover, claudins are frequently overexpressed in malignant cells, in which the mature tight-junction strands are not present. However, claudin's functions in such naive-contact carcinoma cells are not clear. In this study, we describe the physical interaction of ephrin-B1 with claudins. Although ephrin-B1 interacts with claudins on the same cell surface in cis, the tyrosine phosphorylation of the cytoplasmic region of ephrin-B1 was found to be markedly enhanced by the cell–cell contact formation in a manner dependent on claudin. The expression of ephrin-B1 stimulates the paracellular permeability in MDCK cells, which depends on the tyrosine phosphorylation of ephrin-B1. This is the first report to show that the extracellular region of ephrin-B1 associates with a protein other than its receptor in the Eph family kinases. These observations provide further evidence for the possibility that ephrin-B1 inhibits the formation of the tight cell–cell adhesion in a wide variety of epithelial and cancer cells regardless of the existence of cognate Eph receptors. Results Claudin interacts with the extracellular domain (ECD) of ephrin-B1 In the attempt to identify the proteins associating with ephrin-B1, we initially screened cDNA library constructed from Xenopus embryos at an early stage of development by the procedure described in Figure 1A. After several rounds of screening of total 5 × 104 cDNAs, we isolated several independent cDNAs, which encode proteins coimmunoprecipitated with ephrin-B1. Among them, one clone (#12; Figure 1A) was almost identical (97% identical in amino acids) to Xenopus claudin4 (CLD4)L1 through the partial DNA sequencing, which is most closely related to CLD4 in mouse and human (Fujita et al, 2002). Therefore, we focused our study to examine the interaction of ephrin-B1 with CLD4 and also with claudin1 (CLD1), which is most widely expressed in mammalian tissues among the members of claudin family. Figure 1.Ephrin-B1 forms a complex with CLD1 and CLD4: (A) Schematic outline of cloning procedure for the cDNAs encoding proteins associating with ephrin-B1. A detailed explanation is provided in Materials and methods. (Bottom): A plasmid encoding the cDNA (#12) was transiently transfected into COS1 cells together with or without ephrin-B1. The cell lysates were immunoprecipitated (IP) with an anti-ephrin-B1 antibody, and then subjected to IB with anti-myc antibody. It should be noted that six copies of the myc-epitope tagged with the cDNA increases the protein size around 8 kDa. (B) COS1 cells were transiently transfected with a plasmid encoding ephrin-B1 with that encoding flag-tagged CLD1 (lanes 1–4) or CLD4 (lanes 5–8). Cells were lysed and immunoprecipitated with an anti-flag (lanes 1, 5), mouse IgG1 (lanes 2, 6), anti-ephrin-B1 (C18 rabbit, lanes 3, 7) or normal rabbit serum (NRS) (lanes 4, 8). The precipitates were subjected to IB with anti-ephrin-B1 (C18 rabbit) or anti-claudin as indicated. The most upper band of co-precipitated ephrin-B1 at 48 kDa was partly overlapped with the band of immunoglobulin heavy chain (lane 5). The smaller-sized bands recognized with this antibody may be degradated products of ephrin-B1. (C) HT29 cells were lysed and immunoprecipitated (IP) with anti-ephrin-B1 (goat), anti-CLD1, anti-CLD4, NGS, and NRS or mouse IgG1, respectively, as indicated above the lanes. The precipitates were subjected to IB with anti-CLD1, anti-CLD4 or anti-ephrin-B1 (goat). Co-precipitated ephrin-B1 and claudins are indicated by arrowheads. (D) HT29 cells (left) or MDCK ephrin-B1 Tet-ON cells (right) were immunostained with the indicated antibodies. The corresponding computer-reconstructed vertical sections in the X–Z plane are shown. Download figure Download PowerPoint To confirm the physical association between claudins with ephrin-B1, coexpression and immunoprecipitation (IP) analysis was first performed in COS1 cells. It was revealed that ephrin-B1 was co-precipitated with CLD1 by the specific antibody (Figure 1B, lane 1), but not by the normal mouse IgG1 (Figure 1B, lane 2). This result was further confirmed through experiments using the antibodies in reverse order. CLD1 was co-precipitated with ephrin-B1 (Figure 1B, lanes 3 and 4, arrowhead). In addition to CLD1, the stable association of CLD4 with ephrin-B1 was demonstrated using similar experiments (Figure 1B, lanes 5–8). We also examined the association between endogenous claudins and ephrin-B1 using the HT29 colon cancer cell line, where ephrin-B1, CLD1 and CLD4 are highly expressed. CLD1 and CLD4 were co-precipitated with ephrin-B1 from the extract of HT29 cells using the specific antibody, but not by normal goat serum (NGS) (Figure 1C, lanes 1, 2, 5 and 6). Furthermore, ephrin-B1 was co-precipitated with CLD1 or CLD4 using the specific antibodies in HT29 cells, confirming a stable level of interaction among these molecules (Figure 1C, lanes 3, 4, 7, 8). In HT29 cells, claudins and ephrin-B1 were diffusely overlapped in the lateral membrane. On the other hand, in ephrin-B1-expressing MDCK cells, the localization of CLD1 was restricted to the tight junctions where ZO-1 was expressed, while ephrin-B1 showed overlapping, but a wider level of expression along the entire region in the lateral membrane (Figure 1D). In order to determine the region within CLD1, required for the interaction with ephrin-B1, we generated deletion mutants of CLD1. Difference in the binding affinity of the mutants mainly localized at the cell membrane indicates that the cytoplasmic regions of CLD1 were not involved in the interaction with ephrin-B1 (Figure 2A, #1, 6 and 7). Within mutants localized both in the cytoplasm and cell membrane, the ones possessing the first ECD (ECD1) were tightly bound to ephrin-B1 (Figure 2A, #3 and 8), whereas mutants lacking this domain failed to associate with ephrin-B1 (Figure 2A, #2, 4 and 5), suggesting that ECD1 domain of CLD1 is responsible for binding to ephrin-B1. We also generated the same sets of CLD4 mutants with those of CLD1, and revealed that the same domain of CLD4 was necessary for the association with ephrin-B1 (data not shown). These results were further confirmed by the in vitro analysis using GST-tagged CLD1 mutants. The GST-fusion proteins, including each extracellular or cytoplamic domain, were incubated with the lysate of cells expressing ephrin-B1, and the association of the claudin mutant and ephrin-B1 was analyzed. The interaction between ephrin-B1 and CLD1 mutant containing ECD1 (aa 1–103) was confirmed (Figure 2B, GST-ECD1). A similar experiment for GST-tagged ephrin-B1 mutants was also performed. CLD1 interacted with the ECD of ephrin-B1 (ephrin-B1 ECD), but not the transmembrane and cytoplasmic domains (ephrin-B1 cyto) (Figure 2B). Figure 2.ECD of ephrin-B1 and claudins are required for their association: (A) A schematic diagram of the wild-type and deletion mutants of CLD1 used in this study. Proteins are depicted to scale: ECD1 and ECD2, the first and the second ECD; C1 and C2, the first and the second cytoplasmic domain. COS1 cells were transiently transfected with ephrin-B1, with flag-tagged deletion mutants of CLD1. The numbers indicated above the lanes correspond to those in the scheme. The cell lysates were immunoprecipitated (IP) with anti-flag and subjected to IB with anti ephrin-B1 (C18). The localization of each mutant in COS1 cells was examined by immunostaining, and depicted as M (cell membrane) and C (cytoplasm). The mutant #9 was mostly localized in the cytoplasm. (B) The lysates from COS1 cells transiently transfected with ephrin-B1 (top) or CLD1 (bottom) were incubated with GST-fusion protein of CLD1 or ephrin-B1 mutants described in Supplementary data 1. GST-fusion proteins were pulled with glutathione-sepharose beads, and the co-precipitated ephrin-B1 or CLD1 was detected by immunoblot. The input of GST-fusion proteins was shown by rehybridization of the filters with anti-GST antibody. The lower band at 25 kDa was observed in some GST-fusion proteins, which might be the degradated product. (C) COS1 cells were transiently transfected with flag-tagged CLD11−103 with a wild-type or deletion mutant of ephrin-B1. The cell lysates were immunoprecipitated with anti-flag and then immunoblotted with anti-ephrin-B1 (C18) antibody. (D) L ephrin-B1 were either infected with retrovirus containing recombinant CLD4 plasmid (CLD4+) or mock plasmid (CLD4−), and treated with EphB2–Fc or control Fc at 10 μg/ml for 5 min as indicated. After washing, the cells were either lysed for subjecting to the IB with anti-mouse IgG to detect EphB2–Fc, or immunostained with anti-mouse Fc antibody and TOTO-3 iodide (left and right panels, respectively). Download figure Download PowerPoint Within the ECD of ephrin-B1, the most amino-terminal region (aa 1–166), ephrin-B1–N was required for the interaction with CLD1, because CLD1 fragment containing ECD1 (CLD11−103, corresponding to #8 in Figure 2A) binds to ephrin-B1Δ167−236, which lacks a residual region of the ECD of ephrin-B1 (Figure 2C). Moreover, the association of ephrin-B1Δ167−236 and CLD4 fragment containing ECD1 (CLD41−103) was also observed by similar experiments (data not shown). Therefore, we concluded that ephrin-B1–N did interact with the amino-terminal region of claudins, including the first ECD. As ephrin-B1–N also contains a region essential for interaction with EphB receptors (Himanen et al, 2001), we further examined whether EphB2 and claudin competitively bind to ephrin-B1 using EphB2–Fc, which is a fusion protein of the ECD of EphB2 with the Fc region of mouse immunoglobulin. Parent L cells do not express detectable claudins (Furuse et al, 1998); therefore, CLD4 was retrovirally transduced in L cells stably expressing ephrin-B1 (L ephrin-B1). When EphB2–Fc was added in the cell culture medium, expression of CLD4 reduced the amount of membrane-bound EphB2–Fc, which was detected by both immunoblotting (IB) analysis and immunostaining (Figure 2D). Moreover, in L cells expressing both ephrin-B1 and CLD1 (L ephrin-B1 CLD1), ephrin-B1, but not CLD1, was pulled by EphB2–Fc, while CLD1 was coimmunoprecipitated with ephrin-B1 from the same cell lysate (data not shown). Therefore, CLD1-bound ephrin-B1 does not seem to associate with EphB2–Fc. These results suggest that EphB2 and claudins may competitively associate with the ECD of ephrin-B1. Ephrin-B1 is phosphorylated on tyrosine residues in the cytoplasmic domain by the interaction with claudin When ephrin-B1 is stimulated using EphB2, the cytoplasmic domain of ephrin-B1 is highly phosphorylated on tyrosine residues. As the association of claudin and ephrin-B1 depends on their extracellular regions, we subsequently examined whether the phosphorylation of ephrin-B1 was triggered by the interaction with claudin. The coexpression of ephrin-B1 and CLD1 or CLD4 leads to the tyrosine phosphorylation of ephrin-B1 in COS1 cells (Figure 3A, lanes 2 and 5). However, overexpression of CLD1 mutant, which lacks the binding site with ephrin-B1 (CLD1 ΔECD1, corresponds to #2 in Figure 2A), was not able to induce the phosphorylation of ephrin-B1 (Figure 3A, lane 3). Similarly, the corresponding mutant of CLD4 (CLD4 ΔECD1) did not affect the phosphorylation of ephrin-B1 (Figure 3A, lane 6). Moreover, the same result was also obtained using another cell line, HEK 293 cells (Figure 3B). The phosphorylation of ephrin-B1 through interaction with claudins was also confirmed by the reconstitution experiments using L cells, in which ephrin-B1 is endogenously expressed at a low level. The tyrosine phosphorylation of ephrin-B1 was clearly induced in L cells stably expressing CLD1 (L CLD1) or CLD4 (L CLD4) when compared to the parent L cells (Figure 3C). However, ephrin-B1 was not phosphorylated in L cells stably expressing claudins lacking in the first ECD (ΔECD1) or only the ECD1 of CLD1 or CLD4 (corresponding to #8 in Figure 2A). These results indicate that the association of claudin with ephrin-B1 is required for its phosphorylation, but the binding region located in the ECD1 of claudin was insufficient to induce the phosphorylation of ephrin-B1. The phosphorylation of ephrin-B1 by claudin took place on tyrosine residues in its cytoplasmic region, because the phosphorylation was almost completely abolished when four tyrosine residues within the cytoplasmic domain of ephrin-B1 were mutated (ephrin-B1 4YF; Figure 4A). Two tyrosine residues located at the carboxyl-terminus of ephrin-B1 (Tyr 343, 344) were not the major target of claudin-induced phosphorylation, because the phosphorylation level of ephrin-B1 remained almost unchanged following the mutation of these two tyrosines (data not shown). Figure 3.Ephrin-B1 is tyrosine phosphorylated by coexpression with claudins: (A) COS1 cells were transiently transfected with ephrin-B1 together with the wild type or mutant lacking the ΔECD1 of CLD1 (lanes 2, 3) or CLD4 (lanes 5, 6). Cell lysates were immunoprecipitated with anti-ephrin-B1 (goat) and subjected to IB with anti-phosphotyrosine (4G10). (B) HEK293 cells were transiently transfected with the plasmids as indicated. Cell lysates were subjected for the IP and IB as described in (A). (C) The cell lysates from either parent L cell, L cell lines stably expressing the wild type, ΔECD1 or the ECD1 of CLD1 or CLD4 were immunoprecipitated with anti-ephrin-B1 (goat) and subjected to IB with 4G10. Representative results of two independent clones (#1, 2) each are shown. Download figure Download PowerPoint Figure 4.PP2 inhibits the phosphorylation of ephrin-B1 induced by claudins. (A) COS1 cells were transfected with the indicated plasmids. In (B), transfected cells were treated with PP2 or PP3 as described in Materials and methods. Ephrin-B1 was immunoprecipitated from individual cell lysate, and subjected to IB with 4G10. The amino-acid sequence of the carboxyl-tail of human ephrin-B1 is shown at the bottom of (A). The cell lysates were immunoblotted with anti-ephrin-B1 (goat) or anti-claudin antibodies to detect the expression level (bottom). Download figure Download PowerPoint We next examined whether the claudin-induced phosphorylation of ephrin-B1 was caused by Src family kinases (SFK), known to be responsible for the tyrosine phosphorylation of ephrin-B1 by the cognate Eph receptor stimulation. The treatment of the cells with 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2), an inhibitor of the SFK, was seen to almost completely abolish the tyrosine phosphorylation of ephrin-B1 induced by the coexpression of CLD1 or CLD4 (Figure 4B). However, the control 4-amino-7-phenylpyrazol[3,4-d]pyrimidine (PP3) did not exhibit any effect on the tyrosine phosphorylation of ephrin-B1 in claudin-overexpressed cells (Figure 4B). Tyrosine phosphorylation of ephrin-B1 in stable lines of L CLD1 or L CLD4 was also inhibited by the treatment with PP2 (data not shown). These results suggest that ephrin-B1 is phosphorylated on tyrosine residues most probably via the activity of SFK following interaction with claudin. As interaction with EphB2 induces tyrosine phosphorylation of ephrin-B1, which in turn serves as the docking site for the Src homology 2 domain (SH2 domain) of Grb4 (Cowan and Henkemeyer, 2001), we examined whether claudin-induced phosphorylation of ephrin-B1 also induces association with Grb4. The GST fusion containing the SH2 domain of Grb4 was recruited to ephrin-B1 when ephrin-B1 was phosphorylated through coexpression with CLD1 or CLD4 (Supplementary data 2). Phosphorylation of ephrin-B1 is significantly enhanced by cell–cell contact formation The effect of cell–cell contacts on the phosphorylation of ephrin-B1 was then analyzed through the overlay coculture method using stable lines of L cells. L ephrin-B1 were overlaid on the parent cells or either CLD1- or CLD4-expressing cells, while, as a control, L ephrin-B1 cells were overlaid on the EphB2-expressing cells. Although ephrin-B1 was highly phosphorylated through the coculture with cells expressing EphB2, there was no significant phosphorylation of ephrin-B1 by the coculture with L CLD1 or L CLD4 (Supplementary data 3A). These results indicate that claudins do not stimulate the phosphorylation of ephrin-B1 through the mechanism of trans, intercellular association between the two proteins. On the other hand, ephrin-B1 was phosphorylated in L ephrin-B1 CLD1 when cocultured onto CLD1-expressing cells (Supplementary data 3B). Therefore, in response to the intercellular adhesion of claudins, ephrin-B1 may be phosphorylated through the interaction with adjacent claudins in the same cell membrane. We then tested the possibility of ephrin-B1 interacting with claudin in cis on the same cells. In L CLD4, ephrin-B1 associated with CLD4 even when they were plated as scattered single cells (Figure 5A, lane 4). The tyrosine phosphorylation of ephrin-B1 was seen to be greatly influenced by the cell density. Ephrin-B1 was highly phosphorylated when L CLD4 were plated at a high density, while it was reduced when the cells were plated as scattered single cells (Figure 5A, comparing lanes 4 and 6). On the other hand, the phosphorylation level of ephrin-B1 in the parent L cells was low and apparently unchanged by the cell density (Figure 5A, bottom, right). In order to examine the effect of the claudin–claudin trans adhesion accompanied by the cellular contact on the phosphorylation of ephrin-B1, the phosphorylation level of ephrin-B1 was evaluated during the dynamic change of the intercellular adhesion. When cell–cell interaction was completely disrupted by the depletion of calcium in the medium, the tyrosine phosphorylation of ephrin-B1 was also abolished (Figure 5B). Similar results were obtained in L CLD1 (data not shown). On the other hand, the cell dissociation did not modify the phosphorylation level of ephrin-B1 in the parent L cells (Figure 5B, bottom). In order to exclude the possibility that intracellular concentration of calcium may also have been decreased by the cell incubation with ethylene glycol bis (β-aminoethyl ether) N,N,N′,N′-tetraacetic acid (EGTA) and have affected the phosphorylation of ephrin-B1, we performed a control experiment where cells were treated with nifedipine, a blocker of the calcium channel. The decrease in the level of intracellular calcium caused by nifedipine did not affect the status of the association of ephrin-B1 with claudin, nor the phosphorylation of ephrin-B1 (data not shown). Therefore, these results suggest that the phosphorylation of ephrin-B1 through the interaction of claudin was significantly induced by cell–cell contact. Figure 5.The phosphorylation of ephrin-B1 correlates with the cell–cell interactions. (A) Parent L cells (lane 1) or L cells stably expressing CLD4 (L CLD4, lanes 2–7), which were indicated as CLD4 (−) or (+) above the lanes, were grown until confluent. The cells were detached by EGTA, and replated on new dishes either at a low density as scattered single cells or at a high density. Cells were lysed for IP with anti-ephrin-B1 or NGS) which were then subject to IB with 4G10 (pTyr). The membrane was re-hybridized with anti-ephrin-B1 (goat) or anti-CLD4 antibody. The expression of CLD4 in each cell lysate was confirmed by IB (bottom, left). Bottom, right: The tyrosine phosphorylation of ephrin-B1 in parent L cells was examined when the cells were plated as single cells or at a high cell density. (B) L CLD4 (top) or parent L cells (bottom) were grown until confluent. The cells were incubated in HBSS− containing 2 mM EGTA until the majority of the cell–cell contacts were lost, but not detached from the substrate. After washing, monolayers were incubated in the cell culture medium for 4 h. The cells were lysed before (confluent) and after calcium depletion (EGTA), and after changing to the normal cell culture medium for 4 h (restoration) to analyze the phosphorylation of ephrin-B1 as described in (A). Download figure Download PowerPoint The previous conclusion was further supported by the examination of the intracellular localization of tyrosine-phosphorylated ephrin-B1, with the phospho-specific antibody generated against Tyr317 of ephrin-B1. Tyr317 of ephrin-B1 (corresponding to Tyr305 of mouse ephrin-B1) is phosphorylated when stimulated with EphB2, and is a critical requirement for interaction with Grb4 (Bong et al, 2004). In the L ephrin-B1, tyrosine phosphorylation of ephrin-B1 was not observed, regardless of the cell density involved (Figure 6, columns A and C). On the other hand, the L ephrin-B1 CLD1 showed the tyrosine phosphorylation of ephrin-B1 clearly at the cell–cell contact sites, although it was not detected in single isolated cells (Figure 6, columns B and D). The same results were obtained when L CLD4 were used (data not shown), while these observations were further supported by the same analysis using MDCK ephrin-B1 Tet-ON cells, where CLD1 and CLD4 were endogenously expressed (Supplementary data 4). Figure 6.The intercellular interaction of L CLD induced tyrosine phosphorylation of ephrin-B1. (A–D) L ephrin-B1 (columns A, C) or L ephrin-B1 CLD1 (columns B, D) were plated either at a low (columns A, B) or high (columns C, D) cell density. The cells were immunostained with anti-ephrin-B1 (red) and anti-pTyr ephrin-B1 (green) antibodies while the nucleus was stained with TOTO-3 iodide. (E) The expression of cludin1 in L ephrin-B1 CLD1 cells is shown. Download figure Download PowerPoint In the L and MDCK cell lines used in this study, there was no detectable expression of EphB2 exposed through immunoblot analysis (data not shown). The enhancement of the tyrosine phosphorylation of EphB2 was not detected after these cells were treated with ephrin-B1–Fc (Supplementary data 5A). Moreover, when L or MDCK cells were incubated with ephrin-B1–Fc, the membrane-bound ephrin-B1–Fc was not detected following staining with anti-Fc antibody (Supplementary data 5B). The treatment of the cells with an excess volume of soluble ephrin-B1 blocks receptors to interact with membrane-bound ephrin-B1 in trans, thereby shutting off the reverse signaling. On the other hand, the ephrin-B1 phosphorylation level was not attenuated in the L and MDCK cell lines through the addition of ephrin-B1–Fc (Supplementary data 5C), indicating that receptors stimulating ephrin-B1 are not detected in these cells. Phosphorylation of ephrin-B1 is enhanced by ECD of CLD1 In order to evaluate the possibility that claudin–claudin trans interaction enhances the tyrosine phosphorylation of ephrin-B1, CLD1–Fc fusion protein was prepared. For mimicking the trans interaction between claudins mediated by the intercell contacts, the first and the second ECDs of CLD1, which were linked by flag-epitope tag, were fused to the Fc region of immunoglobulin. Preclustered CLD1–Fc attached to HT29 cell membrane, and at least a part of CLD1 in the cell membrane was colocalized with the CLD1–Fc (Figure 7A). Moreover, the endogenous CLD1 protein was co-precipitated with CLD1–Fc (Figure 7B, top). These results indicate that the CLD1–Fc fusion protein effectively binds to the cellular CLD1 at the cell surface. The induction of ephrin-B1 phosphorylation was not evident by adding the preclustered soluble CLD1–Fc in the culture medium of L ephrin-B1 CLD1 (data not shown). On the" @default.
• W2092136345 created "2016-06-24" @default.
• W2092136345 creator A5019744439 @default.
• W2092136345 creator A5039262445 @default.
• W2092136345 creator A5076707760 @default.
• W2092136345 date "2005-10-06" @default.
• W2092136345 modified "2023-09-27" @default.
• W2092136345 title "Phosphorylation of ephrin-B1 via the interaction with claudin following cell–cell contact formation" @default.
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