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• W2092138304 abstract "In this work, we present a novel surface and assay for the simultaneous detection of DNA and protein analytes on a surface plasmon resonance (SPR) imaging sensor. A mixed DNA/oligo (ethylene glycol) (OEG) self-assembled monolayer (SAM) is created using a microarrayer. Thiol-modified single-stranded DNA sequences are spotted onto a gold-coated glass substrate. Backfilling with an OEG-modified alkanethiol creates a protein-resistant surface background. Antibodies conjugated to complementary single-stranded DNA sequences are immobilized on the surface through DNA hybridization. By converting only part of the DNA array into a protein array, simultaneous detections of DNA and protein analytes are possible. A model system of two cDNA sequences and two human pregnancy hormones are used to demonstrate the assay. No cross-reactivity was observed between DNA or protein analytes and nontargeted immobilized cDNA sequence or antibodies. A response from a detection of a single analyte in a mixture of protein and DNA analytes corresponds well with that of a single-analyte solution." @default.
• W2092138304 created "2016-06-24" @default.
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• W2092138304 date "2008-05-06" @default.
• W2092138304 modified "2023-10-17" @default.
• W2092138304 title "Hybrid Surface Platform for the Simultaneous Detection of Proteins and DNAs Using a Surface Plasmon Resonance Imaging Sensor" @default.
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• W2092138304 doi "https://doi.org/10.1021/ac800263j" @default.
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