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• W2092138836 endingPage "270" @default.
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• W2092138836 abstract "Buccal micronucleus cytome (BMCyt) assay monitors genetic damage, cell proliferation and cell death in humans exposed to occupational and environmental agents. BMCyt is used as an indicator of genotoxic exposure, since it is associated with chromosomal instability. There is little research on the occupational exposure among road construction workers for genotoxicity testing. In the present study, we evaluated MN frequencies and other nuclear changes, karyorrhexis (KR), karyolysis (KL), broken egg (BE), binucleate (BN), condensed chromatin cell (CCC), and picnotic cell (PC) in buccal mucosa cells of 40 road construction workers (twenty smokers and twenty non-smokers) and 40 control groups consisting of healthy persons (twenty smokers and twenty non-smokers). Microscopic observation was performed of 2000 cells per individual in both road construction workers and control group. In control and worker groups, for each person repair index (RI) was calculated via formula KR+L/BE+MN. The results showed a statistically significant increase in the frequency of MN in buccal epithelial cells of exposed group compared with control group (p<0.001). There is no significant difference between smokers and non-smokers for incidence of MN or nuclear changes and value of RI in exposed group. In road construction workers, RI is lower than the control group. There is a significant difference between workers and control group (p<0.001) for RI. Our data reveal that asphalt fumes during road paving operations are absorbed by workers and that asphalt fume exposure is able to significantly induce cytogenetic damage in buccal mucosa cells of workers after controlling some possible confounding factors, such as age, sex and smoking habits. In addition to determination of nuclear changes and the micronucleus, the determination of RI value presents a new approach to genotoxic bio-monitoring assessment studies of occupationally exposed population." @default.
• W2092138836 created "2016-06-24" @default.
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• W2092138836 date "2013-06-01" @default.
• W2092138836 modified "2023-10-18" @default.
• W2092138836 title "Bio-monitoring for the genotoxic assessment in road construction workers as determined by the buccal micronucleus cytome assay" @default.
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• W2092138836 doi "https://doi.org/10.1016/j.ecoenv.2013.01.030" @default.
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