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• W2092139739 abstract "Plasminogen activation is observed in the human epidermis during re‐epithelialization of epidermal defects. The activation reaction depends on plasminogen activators (PAs) associated with re‐epithelializing keratinocytes. PA inhibitor type 2 (PAI‐2) is thought to be a major epidermal PA inhibitor in keratinocytes. However, no data are available on the expression of PAI‐2 in keratinocytes during epidermal regeneration. We have therefore analysed PAI‐2 at the mRNA and protein level in keratinocyte cultures as well as in epidermal lesions in which re‐epithelializing keratinocytes were apparent. We found that PAI‐2 expression at the mRNA and protein level was negatively correlated with the cell density in regular keratinocyte cultures. In organotypic cocultures, in which the transition from a re‐epithelializing to a sedentary phenotype can be studied, PAI‐2 was most strongly expressed in early cultures prior to formation of a differentiated epidermis‐like structure. We found a strong expression of PAI‐2 in keratinocytes that re‐epithelialized dermal burn wounds or lesions caused by the autoimmune blistering disease pemphigus vulgaris. Our results suggest that not only PAs, but also a major PA inhibitor, PAI‐2, are expressed in keratinocytes that are actively involved in re‐epithelialization." @default.
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• W2092139739 date "1998-01-01" @default.
• W2092139739 modified "2023-09-27" @default.
• W2092139739 title "Plasminogen activator inhibitor type 2 is expressed in keratinocytes during re-epithelialization of epidermal defects" @default.
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• W2092139739 doi "https://doi.org/10.1046/j.1365-2133.1998.02021.x" @default.
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