FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092153005> ?p ?o ?g. }

• W2092153005 endingPage "43" @default.
• W2092153005 startingPage "35" @default.
• W2092153005 abstract "The spirolides are marine toxins that belong to a new class of macrocyclic imines produced by dinoflagellates. In this study a previously described solid-phase receptor-based assay for the detection of spirolides was optimized for high-throughput screening and prevalidated. This method is based on the competition between 13-desmethyl spirolide C and biotin-α-bungarotoxin immobilized on a streptavidin-coated surface, for binding to nicotinic acetylcholine receptors. In this inhibition assay the amount of nAChR bound to the well surface is quantified using a specific antibody, followed by a second anti-mouse IgG antibody labeled with horseradish peroxidase (HRP). The assay protocol was optimized for 384-well microplates, which allowed a reduction of the amount of reagents per sample and an increase of the number of samples per plate versus previously published receptor-based assays. The sensitivity of the assay for 13-desmethyl spirolide C ranged from 5 to 150 ng mL−1. The performance of the assay in scallop extracts was adequate, with an estimated detection limit for 13-desmethyl spirolide C of 50 μg kg−1 of shellfish meat. The recovery rate of 13-desmethyl spirolide C for spiked samples with this assay was 80% and the inter-assay coefficient of variation was 8%. This 384-well microplate, chemiluminescence method can be used as a high-throughput screening assay to detect 13-desmethyl spirolide C in shellfish meat in order to reduce the number of samples to be processed through bioassays or analytical methods." @default.
• W2092153005 created "2016-06-24" @default.
• W2092153005 creator A5010568577 @default.
• W2092153005 creator A5030884506 @default.
• W2092153005 creator A5041762507 @default.
• W2092153005 creator A5058883724 @default.
• W2092153005 creator A5077293613 @default.
• W2092153005 date "2013-12-01" @default.
• W2092153005 modified "2023-10-17" @default.
• W2092153005 title "High-throughput receptor-based assay for the detection of spirolides by chemiluminescence" @default.
• W2092153005 cites W1967943171 @default.
• W2092153005 cites W1979930136 @default.
• W2092153005 cites W1982362768 @default.
• W2092153005 cites W1982626639 @default.
• W2092153005 cites W1985601577 @default.
• W2092153005 cites W1986703633 @default.
• W2092153005 cites W1988266357 @default.
• W2092153005 cites W2010768663 @default.
• W2092153005 cites W2011088407 @default.
• W2092153005 cites W2022256286 @default.
• W2092153005 cites W2025158924 @default.
• W2092153005 cites W2029922432 @default.
• W2092153005 cites W2030938378 @default.
• W2092153005 cites W2033750834 @default.
• W2092153005 cites W2034818688 @default.
• W2092153005 cites W2039851274 @default.
• W2092153005 cites W2042890168 @default.
• W2092153005 cites W2043616279 @default.
• W2092153005 cites W2047349482 @default.
• W2092153005 cites W2047584744 @default.
• W2092153005 cites W2049866908 @default.
• W2092153005 cites W2067691944 @default.
• W2092153005 cites W2069855932 @default.
• W2092153005 cites W2070521358 @default.
• W2092153005 cites W2082320022 @default.
• W2092153005 cites W2094562639 @default.
• W2092153005 cites W2103369617 @default.
• W2092153005 cites W2105613642 @default.
• W2092153005 cites W2110879032 @default.
• W2092153005 cites W2120584765 @default.
• W2092153005 cites W2145763588 @default.
• W2092153005 cites W2322316455 @default.
• W2092153005 doi "https://doi.org/10.1016/j.toxicon.2013.06.015" @default.
• W2092153005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23827412" @default.
• W2092153005 hasPublicationYear "2013" @default.
• W2092153005 type Work @default.
• W2092153005 sameAs 2092153005 @default.
• W2092153005 citedByCount "14" @default.
• W2092153005 countsByYear W20921530052014 @default.
• W2092153005 countsByYear W20921530052015 @default.
• W2092153005 countsByYear W20921530052017 @default.
• W2092153005 countsByYear W20921530052021 @default.
• W2092153005 countsByYear W20921530052022 @default.
• W2092153005 crossrefType "journal-article" @default.
• W2092153005 hasAuthorship W2092153005A5010568577 @default.
• W2092153005 hasAuthorship W2092153005A5030884506 @default.
• W2092153005 hasAuthorship W2092153005A5041762507 @default.
• W2092153005 hasAuthorship W2092153005A5058883724 @default.
• W2092153005 hasAuthorship W2092153005A5077293613 @default.
• W2092153005 hasConcept C104488531 @default.
• W2092153005 hasConcept C119128265 @default.
• W2092153005 hasConcept C145802702 @default.
• W2092153005 hasConcept C170493617 @default.
• W2092153005 hasConcept C185592680 @default.
• W2092153005 hasConcept C2777367657 @default.
• W2092153005 hasConcept C2777477808 @default.
• W2092153005 hasConcept C2780837634 @default.
• W2092153005 hasConcept C2910844273 @default.
• W2092153005 hasConcept C43617362 @default.
• W2092153005 hasConcept C54355233 @default.
• W2092153005 hasConcept C55493867 @default.
• W2092153005 hasConcept C86803240 @default.
• W2092153005 hasConceptScore W2092153005C104488531 @default.
• W2092153005 hasConceptScore W2092153005C119128265 @default.
• W2092153005 hasConceptScore W2092153005C145802702 @default.
• W2092153005 hasConceptScore W2092153005C170493617 @default.
• W2092153005 hasConceptScore W2092153005C185592680 @default.
• W2092153005 hasConceptScore W2092153005C2777367657 @default.
• W2092153005 hasConceptScore W2092153005C2777477808 @default.
• W2092153005 hasConceptScore W2092153005C2780837634 @default.
• W2092153005 hasConceptScore W2092153005C2910844273 @default.
• W2092153005 hasConceptScore W2092153005C43617362 @default.
• W2092153005 hasConceptScore W2092153005C54355233 @default.
• W2092153005 hasConceptScore W2092153005C55493867 @default.
• W2092153005 hasConceptScore W2092153005C86803240 @default.
• W2092153005 hasLocation W20921530051 @default.
• W2092153005 hasLocation W20921530052 @default.
• W2092153005 hasLocation W20921530053 @default.
• W2092153005 hasLocation W20921530054 @default.
• W2092153005 hasOpenAccess W2092153005 @default.
• W2092153005 hasPrimaryLocation W20921530051 @default.
• W2092153005 hasRelatedWork W2004407634 @default.
• W2092153005 hasRelatedWork W2019871631 @default.
• W2092153005 hasRelatedWork W2042078005 @default.
• W2092153005 hasRelatedWork W2049866908 @default.
• W2092153005 hasRelatedWork W2077592736 @default.
• W2092153005 hasRelatedWork W2158591239 @default.
• W2092153005 hasRelatedWork W2355703899 @default.

• next