FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092163049> ?p ?o ?g. }

• W2092163049 endingPage "7032" @default.
• W2092163049 startingPage "7028" @default.
• W2092163049 abstract "A system for molecular cloning in Streptococcus pneumoniae was developed. The multicopy plasmids pMV158 (5.4 kilobases) and pLS1 (4.3 kilobases), which confer tetracycline resistance, were used as vectors to clone chromosomal genes of S. pneumoniae in host cells of this species. A 3.3-kilobase restriction fragment containing the malM gene, which codes for amylomaltase, was cloned in a deletion mutant lacking chromosomal homology with the fragment. The recombinant plasmid pLS70, could transform over 50% of a recipient population to maltose utilization. Amylomaltase constituted up to 10% of the protein of cells containing pLS70. A derivative with a deletion, pLS69, appeared to gain a selective advantage by producing less enzyme. A 10-kilobase restriction fragment containing the sul-d gene for sulfonamide resistance was cloned in the presence of the homologous chromosomal gene. De novo establishment of a recombinant plasmid was just as frequent as transformation in an endogenous plasmid. Despite the processing of DNA during uptake in the transformation of S. pneumoniae, recombinant plasmids can be introduced. Models for the reconstruction of recombinant DNA in cells of S. pneumoniae and Bacillus subtilis are considered and compared." @default.
• W2092163049 created "2016-06-24" @default.
• W2092163049 creator A5001922918 @default.
• W2092163049 creator A5015474178 @default.
• W2092163049 creator A5029735094 @default.
• W2092163049 creator A5066711727 @default.
• W2092163049 date "1981-11-01" @default.
• W2092163049 modified "2023-09-29" @default.
• W2092163049 title "Cloning of chromosomal genes in Streptococcus pneumoniae." @default.
• W2092163049 cites W1501133092 @default.
• W2092163049 cites W1517610680 @default.
• W2092163049 cites W1630678156 @default.
• W2092163049 cites W1672321129 @default.
• W2092163049 cites W1968404152 @default.
• W2092163049 cites W1972531948 @default.
• W2092163049 cites W1972741181 @default.
• W2092163049 cites W1979170668 @default.
• W2092163049 cites W1979197786 @default.
• W2092163049 cites W1986722371 @default.
• W2092163049 cites W1989293863 @default.
• W2092163049 cites W1990604707 @default.
• W2092163049 cites W1995814439 @default.
• W2092163049 cites W1999485221 @default.
• W2092163049 cites W2013672165 @default.
• W2092163049 cites W2019410656 @default.
• W2092163049 cites W2040928665 @default.
• W2092163049 cites W2043860143 @default.
• W2092163049 cites W2049445962 @default.
• W2092163049 cites W2051079268 @default.
• W2092163049 cites W2079347416 @default.
• W2092163049 cites W2125520361 @default.
• W2092163049 cites W308347375 @default.
• W2092163049 doi "https://doi.org/10.1073/pnas.78.11.7028" @default.
• W2092163049 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/349187" @default.
• W2092163049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6273903" @default.
• W2092163049 hasPublicationYear "1981" @default.
• W2092163049 type Work @default.
• W2092163049 sameAs 2092163049 @default.
• W2092163049 citedByCount "103" @default.
• W2092163049 countsByYear W20921630492012 @default.
• W2092163049 countsByYear W20921630492013 @default.
• W2092163049 countsByYear W20921630492014 @default.
• W2092163049 countsByYear W20921630492015 @default.
• W2092163049 countsByYear W20921630492017 @default.
• W2092163049 countsByYear W20921630492018 @default.
• W2092163049 countsByYear W20921630492019 @default.
• W2092163049 countsByYear W20921630492021 @default.
• W2092163049 crossrefType "journal-article" @default.
• W2092163049 hasAuthorship W2092163049A5001922918 @default.
• W2092163049 hasAuthorship W2092163049A5015474178 @default.
• W2092163049 hasAuthorship W2092163049A5029735094 @default.
• W2092163049 hasAuthorship W2092163049A5066711727 @default.
• W2092163049 hasBestOaLocation W20921630491 @default.
• W2092163049 hasConcept C104317684 @default.
• W2092163049 hasConcept C121050878 @default.
• W2092163049 hasConcept C128319531 @default.
• W2092163049 hasConcept C150194340 @default.
• W2092163049 hasConcept C153911025 @default.
• W2092163049 hasConcept C19924922 @default.
• W2092163049 hasConcept C199360897 @default.
• W2092163049 hasConcept C204241405 @default.
• W2092163049 hasConcept C22744801 @default.
• W2092163049 hasConcept C40767141 @default.
• W2092163049 hasConcept C41008148 @default.
• W2092163049 hasConcept C54355233 @default.
• W2092163049 hasConcept C552990157 @default.
• W2092163049 hasConcept C86803240 @default.
• W2092163049 hasConceptScore W2092163049C104317684 @default.
• W2092163049 hasConceptScore W2092163049C121050878 @default.
• W2092163049 hasConceptScore W2092163049C128319531 @default.
• W2092163049 hasConceptScore W2092163049C150194340 @default.
• W2092163049 hasConceptScore W2092163049C153911025 @default.
• W2092163049 hasConceptScore W2092163049C19924922 @default.
• W2092163049 hasConceptScore W2092163049C199360897 @default.
• W2092163049 hasConceptScore W2092163049C204241405 @default.
• W2092163049 hasConceptScore W2092163049C22744801 @default.
• W2092163049 hasConceptScore W2092163049C40767141 @default.
• W2092163049 hasConceptScore W2092163049C41008148 @default.
• W2092163049 hasConceptScore W2092163049C54355233 @default.
• W2092163049 hasConceptScore W2092163049C552990157 @default.
• W2092163049 hasConceptScore W2092163049C86803240 @default.
• W2092163049 hasIssue "11" @default.
• W2092163049 hasLocation W20921630491 @default.
• W2092163049 hasLocation W20921630492 @default.
• W2092163049 hasLocation W20921630493 @default.
• W2092163049 hasLocation W20921630494 @default.
• W2092163049 hasOpenAccess W2092163049 @default.
• W2092163049 hasPrimaryLocation W20921630491 @default.
• W2092163049 hasRelatedWork W2350280039 @default.
• W2092163049 hasRelatedWork W2353268965 @default.
• W2092163049 hasRelatedWork W2359665887 @default.
• W2092163049 hasRelatedWork W2367091470 @default.
• W2092163049 hasRelatedWork W2369578102 @default.
• W2092163049 hasRelatedWork W2376603524 @default.
• W2092163049 hasRelatedWork W2379486258 @default.
• W2092163049 hasRelatedWork W2384105220 @default.
• W2092163049 hasRelatedWork W286469573 @default.
• W2092163049 hasRelatedWork W30564900 @default.

• next