Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092173694> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2092173694 endingPage "12026" @default.
- W2092173694 startingPage "12019" @default.
- W2092173694 abstract "Recoverin is an N-myristoylated Ca2+-binding protein that serves as a Ca2+-sensor in visual transduction. We studied the dynamics of its Ca2+-dependent membrane association which depends on the myristoyl modification (Ca2+−myristoyl switch) by surface plasmon resonance spectroscopy. Either recoverin or phospholipid vesicles were immobilized on a sensor chip surface, and the respective binding partner was supplied in the mobile phase. Binding of recoverin to artificial liposomes or rod outer segment membranes was strictly dependent on Ca2+ and the myristoyl group. The Ca2+−myristoyl switch was half-maximal between 4.0 and 7.7 μM Ca2+, depending on whether recoverin or liposomes were in the mobile phase. At saturating [Ca2+], the dissociation constant (KD) of recoverin for phospholipid liposomes was approximately 150 μM. The association and dissociation of recoverin to membranes was fast and biphasic (fast and slow components) with time constants on the order of 0.1 s-1 and 0.01 s-1, respectively. Dissociation of the recoverin−membrane complex was 3-fold faster at low than at high free [Ca2+]. We discuss the analogy between the liposome−sensor chip and the disk surface and conclude that a minor fraction of the total recoverin in a rod outer segment is associated with membranes at resting dark levels of free [Ca2+]." @default.
- W2092173694 created "2016-06-24" @default.
- W2092173694 creator A5005730211 @default.
- W2092173694 creator A5057357802 @default.
- W2092173694 date "1997-10-01" @default.
- W2092173694 modified "2023-10-16" @default.
- W2092173694 title "Calcium-Dependent Binding of Recoverin to Membranes Monitored by Surface Plasmon Resonance Spectroscopy in Real Time" @default.
- W2092173694 cites W1513897103 @default.
- W2092173694 cites W1558733231 @default.
- W2092173694 cites W1562947759 @default.
- W2092173694 cites W1583035666 @default.
- W2092173694 cites W2005871591 @default.
- W2092173694 cites W2016764094 @default.
- W2092173694 cites W2037817929 @default.
- W2092173694 cites W2046292720 @default.
- W2092173694 cites W2064693369 @default.
- W2092173694 cites W2071606867 @default.
- W2092173694 cites W2073513922 @default.
- W2092173694 cites W2082411432 @default.
- W2092173694 cites W2115201271 @default.
- W2092173694 cites W2162692258 @default.
- W2092173694 doi "https://doi.org/10.1021/bi970938d" @default.
- W2092173694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9315839" @default.
- W2092173694 hasPublicationYear "1997" @default.
- W2092173694 type Work @default.
- W2092173694 sameAs 2092173694 @default.
- W2092173694 citedByCount "68" @default.
- W2092173694 countsByYear W20921736942012 @default.
- W2092173694 countsByYear W20921736942013 @default.
- W2092173694 countsByYear W20921736942014 @default.
- W2092173694 countsByYear W20921736942016 @default.
- W2092173694 countsByYear W20921736942017 @default.
- W2092173694 countsByYear W20921736942018 @default.
- W2092173694 countsByYear W20921736942019 @default.
- W2092173694 countsByYear W20921736942021 @default.
- W2092173694 countsByYear W20921736942022 @default.
- W2092173694 crossrefType "journal-article" @default.
- W2092173694 hasAuthorship W2092173694A5005730211 @default.
- W2092173694 hasAuthorship W2092173694A5057357802 @default.
- W2092173694 hasConcept C106847996 @default.
- W2092173694 hasConcept C12554922 @default.
- W2092173694 hasConcept C155672457 @default.
- W2092173694 hasConcept C170493617 @default.
- W2092173694 hasConcept C171250308 @default.
- W2092173694 hasConcept C185154212 @default.
- W2092173694 hasConcept C185592680 @default.
- W2092173694 hasConcept C192562407 @default.
- W2092173694 hasConcept C202033177 @default.
- W2092173694 hasConcept C2778918659 @default.
- W2092173694 hasConcept C2780827179 @default.
- W2092173694 hasConcept C2781139644 @default.
- W2092173694 hasConcept C41625074 @default.
- W2092173694 hasConcept C55493867 @default.
- W2092173694 hasConcept C74998103 @default.
- W2092173694 hasConcept C77207865 @default.
- W2092173694 hasConcept C86803240 @default.
- W2092173694 hasConceptScore W2092173694C106847996 @default.
- W2092173694 hasConceptScore W2092173694C12554922 @default.
- W2092173694 hasConceptScore W2092173694C155672457 @default.
- W2092173694 hasConceptScore W2092173694C170493617 @default.
- W2092173694 hasConceptScore W2092173694C171250308 @default.
- W2092173694 hasConceptScore W2092173694C185154212 @default.
- W2092173694 hasConceptScore W2092173694C185592680 @default.
- W2092173694 hasConceptScore W2092173694C192562407 @default.
- W2092173694 hasConceptScore W2092173694C202033177 @default.
- W2092173694 hasConceptScore W2092173694C2778918659 @default.
- W2092173694 hasConceptScore W2092173694C2780827179 @default.
- W2092173694 hasConceptScore W2092173694C2781139644 @default.
- W2092173694 hasConceptScore W2092173694C41625074 @default.
- W2092173694 hasConceptScore W2092173694C55493867 @default.
- W2092173694 hasConceptScore W2092173694C74998103 @default.
- W2092173694 hasConceptScore W2092173694C77207865 @default.
- W2092173694 hasConceptScore W2092173694C86803240 @default.
- W2092173694 hasIssue "40" @default.
- W2092173694 hasLocation W20921736941 @default.
- W2092173694 hasLocation W20921736942 @default.
- W2092173694 hasOpenAccess W2092173694 @default.
- W2092173694 hasPrimaryLocation W20921736941 @default.
- W2092173694 hasRelatedWork W124525801 @default.
- W2092173694 hasRelatedWork W1980040967 @default.
- W2092173694 hasRelatedWork W1999554227 @default.
- W2092173694 hasRelatedWork W2001191747 @default.
- W2092173694 hasRelatedWork W2009240753 @default.
- W2092173694 hasRelatedWork W2321082801 @default.
- W2092173694 hasRelatedWork W2407183842 @default.
- W2092173694 hasRelatedWork W2728476182 @default.
- W2092173694 hasRelatedWork W3119773807 @default.
- W2092173694 hasRelatedWork W3177316806 @default.
- W2092173694 hasVolume "36" @default.
- W2092173694 isParatext "false" @default.
- W2092173694 isRetracted "false" @default.
- W2092173694 magId "2092173694" @default.
- W2092173694 workType "article" @default.