Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092183694> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2092183694 endingPage "556" @default.
- W2092183694 startingPage "550" @default.
- W2092183694 abstract "In Xanthomonas campestris pv. campestris (Xcc), the chromosomally encoded class A β-lactamase (Bla(xcc)) is expressed at a high basal level in the absence of an inducer and its expression is inducible by ampicillin. Like most of the class A β-lactamases, Bla(xcc) cannot digest the β-lactam ring of cefoxitin. However, Xcc exhibits high basal resistance to cefoxitin. A promoter activity assay with P(blaxcc)-lacZ transcriptional fusion from a plasmid and western blotting using anti-Bla(xcc) polyclonal antibodies demonstrated that a sublethal concentration of cefoxitin can induce expression of the bla(xcc) gene. Cefoxitin can be used as an inducer to study bla(xcc) expression in bla(xcc)-deficient mutants such as Xcbla and XcampR. Addition of cefoxitin to the Bla enzyme solution blocks β-lactamase activity, suggesting that cefoxitin is an inhibitor of Bla(xcc). This explains why there is no β-lactamase activity in cefoxitin-induced Xcc. A significant synergistic effect was also observed between cefoxitin and other β-lactam antibiotics. A homology model demonstrated that the methoxy-group in the β-lactam ring of cefoxitin tends to displace the conserved catalytic water molecule into the active cavity of Bla(xcc), thus leading to formation of a stable but inactive acyl-Bla(xcc) intermediate." @default.
- W2092183694 created "2016-06-24" @default.
- W2092183694 creator A5018669587 @default.
- W2092183694 creator A5045410024 @default.
- W2092183694 creator A5054461516 @default.
- W2092183694 creator A5070344536 @default.
- W2092183694 date "2012-09-01" @default.
- W2092183694 modified "2023-09-26" @default.
- W2092183694 title "Cefoxitin is both an inhibitor of class A beta-lactamase of Xanthomonas campestris pv. campestris str. 17 and an inducer of its gene" @default.
- W2092183694 cites W1501315577 @default.
- W2092183694 cites W1677761592 @default.
- W2092183694 cites W1701997017 @default.
- W2092183694 cites W1861263514 @default.
- W2092183694 cites W1978232051 @default.
- W2092183694 cites W1987167645 @default.
- W2092183694 cites W1988921452 @default.
- W2092183694 cites W1990204840 @default.
- W2092183694 cites W1992456283 @default.
- W2092183694 cites W199299476 @default.
- W2092183694 cites W2033123159 @default.
- W2092183694 cites W2047709322 @default.
- W2092183694 cites W2050456292 @default.
- W2092183694 cites W2057808619 @default.
- W2092183694 cites W2063077890 @default.
- W2092183694 cites W2079362194 @default.
- W2092183694 cites W2082560937 @default.
- W2092183694 cites W2094836436 @default.
- W2092183694 cites W2099303284 @default.
- W2092183694 cites W2115692469 @default.
- W2092183694 cites W2143874510 @default.
- W2092183694 cites W2148940705 @default.
- W2092183694 cites W2156593493 @default.
- W2092183694 cites W2157343730 @default.
- W2092183694 doi "https://doi.org/10.1016/j.resmic.2012.08.003" @default.
- W2092183694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22910283" @default.
- W2092183694 hasPublicationYear "2012" @default.
- W2092183694 type Work @default.
- W2092183694 sameAs 2092183694 @default.
- W2092183694 citedByCount "6" @default.
- W2092183694 countsByYear W20921836942013 @default.
- W2092183694 countsByYear W20921836942014 @default.
- W2092183694 countsByYear W20921836942018 @default.
- W2092183694 countsByYear W20921836942019 @default.
- W2092183694 countsByYear W20921836942020 @default.
- W2092183694 countsByYear W20921836942023 @default.
- W2092183694 crossrefType "journal-article" @default.
- W2092183694 hasAuthorship W2092183694A5018669587 @default.
- W2092183694 hasAuthorship W2092183694A5045410024 @default.
- W2092183694 hasAuthorship W2092183694A5054461516 @default.
- W2092183694 hasAuthorship W2092183694A5070344536 @default.
- W2092183694 hasConcept C104317684 @default.
- W2092183694 hasConcept C143065580 @default.
- W2092183694 hasConcept C153911025 @default.
- W2092183694 hasConcept C2776124114 @default.
- W2092183694 hasConcept C2779489039 @default.
- W2092183694 hasConcept C2780679087 @default.
- W2092183694 hasConcept C2780746887 @default.
- W2092183694 hasConcept C523546767 @default.
- W2092183694 hasConcept C54355233 @default.
- W2092183694 hasConcept C55493867 @default.
- W2092183694 hasConcept C86803240 @default.
- W2092183694 hasConcept C89423630 @default.
- W2092183694 hasConceptScore W2092183694C104317684 @default.
- W2092183694 hasConceptScore W2092183694C143065580 @default.
- W2092183694 hasConceptScore W2092183694C153911025 @default.
- W2092183694 hasConceptScore W2092183694C2776124114 @default.
- W2092183694 hasConceptScore W2092183694C2779489039 @default.
- W2092183694 hasConceptScore W2092183694C2780679087 @default.
- W2092183694 hasConceptScore W2092183694C2780746887 @default.
- W2092183694 hasConceptScore W2092183694C523546767 @default.
- W2092183694 hasConceptScore W2092183694C54355233 @default.
- W2092183694 hasConceptScore W2092183694C55493867 @default.
- W2092183694 hasConceptScore W2092183694C86803240 @default.
- W2092183694 hasConceptScore W2092183694C89423630 @default.
- W2092183694 hasIssue "8" @default.
- W2092183694 hasLocation W20921836941 @default.
- W2092183694 hasLocation W20921836942 @default.
- W2092183694 hasOpenAccess W2092183694 @default.
- W2092183694 hasPrimaryLocation W20921836941 @default.
- W2092183694 hasRelatedWork W2018439630 @default.
- W2092183694 hasRelatedWork W2025478358 @default.
- W2092183694 hasRelatedWork W2047897201 @default.
- W2092183694 hasRelatedWork W2092183694 @default.
- W2092183694 hasRelatedWork W2150245203 @default.
- W2092183694 hasRelatedWork W2150953285 @default.
- W2092183694 hasRelatedWork W2246187118 @default.
- W2092183694 hasRelatedWork W2391151410 @default.
- W2092183694 hasRelatedWork W2410812619 @default.
- W2092183694 hasRelatedWork W27899412 @default.
- W2092183694 hasVolume "163" @default.
- W2092183694 isParatext "false" @default.
- W2092183694 isRetracted "false" @default.
- W2092183694 magId "2092183694" @default.
- W2092183694 workType "article" @default.