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- W2092186656 endingPage "849" @default.
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- W2092186656 abstract "Self-splicing group I introns, like other large catalytic RNAs, contain structural domains. Although the crystal structure of one of these domains has been determined by x-ray analysis, its connection to the other major domain that contains the guanosine-binding site has not been known. Site-directed mutagenesis and kinetic analysis of RNA splicing were used to identify a base triple in the conserved core of both a cyanobacterial ( Anabaena ) and a eukaryotic ( Tetrahymena ) group I intron. This long-range interaction connects a sequence adjacent to the guanosine-binding site with the domain implicated in coordinating the 5′ splice site helix, and it thereby contributes to formation of the active site. The resulting five-strand junction, in which a short helix forms base triples with three separate strands in the Tetrahymena intron, reveals exceptionally dense packing of RNA." @default.
- W2092186656 created "2016-06-24" @default.
- W2092186656 creator A5017573996 @default.
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- W2092186656 date "1997-02-07" @default.
- W2092186656 modified "2023-09-26" @default.
- W2092186656 title "Joining the Two Domains of a Group I Ribozyme to Form the Catalytic Core" @default.
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- W2092186656 doi "https://doi.org/10.1126/science.275.5301.847" @default.
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