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- W2092198906 abstract "CaM labeled with a fluorescent triazinylaniline (TA) derivative at Lys-75 shows 2 species upon binding to PMCA or to the CaM-binding peptide from PMCA (C28). The 1st, transient, species is slightly more fluorescent than the free CaM, while the 2nd, stable, species is much less fluorescent. The 1st species can also be emulated in a stable form by binding TA-CaM to a shorter peptide, C20. The fluorescence of TA derivatives is quenched and red-shifted by polarizable solvents such as water. TANMe2 has an emission maximum of 391 nm in a non-polar solvent (toluene), which is red-shifted to 419 nm in ethanol (permittivity = 24.5). The emission maxima of TACaM-C20 and TACaM-C28 are 409 nm and 421 nm respectively. Using the Lippert equation, we find that the effective permittivity that TA sees in TACaM-C20 is about 5 and in TACaM-C28 is about 30. Structures of TACaM-C20 (based on 1CFF) and TACaM-C28 (based on our new NMR data on CaM-C28) were made. The C20 complex has the TA residue surrounded by the extended CaM molecule, in an environment containing relatively little water. In the C28 complex the CaM molecule is collapsed. The surroundings of the TA residue are calculated from these molecular structures of hydrated TA-CaM, and the results are comparable with the experimental fluorescence data. (Supported by grants TW06837 and NS51769 from the NIH)" @default.
- W2092198906 created "2016-06-24" @default.
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- W2092198906 date "2009-02-01" @default.
- W2092198906 modified "2023-09-28" @default.
- W2092198906 title "Collapse of TA-Calmodulin (TACaM) upon Binding to Ca2+ Pump Peptide C28 Exposes the TA Moiety to Water and Quenches Its Fluorescence" @default.
- W2092198906 doi "https://doi.org/10.1016/j.bpj.2008.12.638" @default.
- W2092198906 hasPublicationYear "2009" @default.
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