FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092201501> ?p ?o ?g. }

• W2092201501 abstract "Sessile bivalves of the genus Mytilus are suspension feeders relatively tolerant to a wide range of environmental changes, used as sentinels in ecotoxicological investigations and marketed worldwide as seafood. Mortality events caused by infective agents and parasites apparently occur less in mussels than in other bivalves but the molecular basis of such evidence is unknown. The arrangement of Mytibase, interactive catalogue of 7,112 transcripts of M. galloprovincialis, offered us the opportunity to look for gene sequences relevant to the host defences, in particular the innate immunity related genes. We have explored and described the Mytibase sequence clusters and singletons having a putative role in recognition, intracellular signalling, and neutralization of potential pathogens in M. galloprovincialis. Automatically assisted searches of protein signatures and manually cured sequence analysis confirmed the molecular diversity of recognition/effector molecules such as the antimicrobial peptides and many carbohydrate binding proteins. Molecular motifs identifying complement C1q, C-type lectins and fibrinogen-like transcripts emerged as the most abundant in the Mytibase collection whereas, conversely, sequence motifs denoting the regulatory cytokine MIF and cytokine-related transcripts represent singular and unexpected findings. Using a cross-search strategy, 1,820 putatively immune-related sequences were selected to design oligonucleotide probes and define a species-specific Immunochip (DNA microarray). The Immunochip performance was tested with hemolymph RNAs from mussels injected with Vibrio splendidus at 3 and 48 hours post-treatment. A total of 143 and 262 differentially expressed genes exemplify the early and late hemocyte response of the Vibrio-challenged mussels, respectively, with AMP trends confirmed by qPCR and clear modulation of interrelated signalling pathways. The Mytibase collection is rich in gene transcripts modulated in response to antigenic stimuli and represents an interesting window for looking at the mussel immunome (transcriptomes mediating the mussel response to non-self or abnormal antigens). On this basis, we have defined a new microarray platform, a mussel Immunochip, as a flexible tool for the experimental validation of immune-candidate sequences, and tested its performance on Vibrio-activated mussel hemocytes. The microarray platform and related expression data can be regarded as a step forward in the study of the adaptive response of the Mytilus species to an evolving microbial world." @default.
• W2092201501 created "2016-06-24" @default.
• W2092201501 creator A5005565452 @default.
• W2092201501 creator A5005694591 @default.
• W2092201501 creator A5006562505 @default.
• W2092201501 creator A5019602062 @default.
• W2092201501 creator A5036392113 @default.
• W2092201501 creator A5037546237 @default.
• W2092201501 creator A5044217748 @default.
• W2092201501 creator A5049654977 @default.
• W2092201501 creator A5061485833 @default.
• W2092201501 creator A5063586699 @default.
• W2092201501 creator A5079802146 @default.
• W2092201501 date "2011-01-26" @default.
• W2092201501 modified "2023-10-09" @default.
• W2092201501 title "Insights into the innate immunity of the Mediterranean mussel Mytilus galloprovincialis" @default.
• W2092201501 cites W1500350383 @default.
• W2092201501 cites W1526394832 @default.
• W2092201501 cites W1964561957 @default.
• W2092201501 cites W1965488138 @default.
• W2092201501 cites W1967361018 @default.
• W2092201501 cites W1968948521 @default.
• W2092201501 cites W1970075546 @default.
• W2092201501 cites W1974584611 @default.
• W2092201501 cites W1975334114 @default.
• W2092201501 cites W1978144128 @default.
• W2092201501 cites W1979023388 @default.
• W2092201501 cites W1980799612 @default.
• W2092201501 cites W1982825103 @default.
• W2092201501 cites W1983927947 @default.
• W2092201501 cites W1984687050 @default.
• W2092201501 cites W1986029366 @default.
• W2092201501 cites W1987015602 @default.
• W2092201501 cites W1987979294 @default.
• W2092201501 cites W1988123382 @default.
• W2092201501 cites W1988365727 @default.
• W2092201501 cites W1988390135 @default.
• W2092201501 cites W1990476456 @default.
• W2092201501 cites W1999873256 @default.
• W2092201501 cites W1999881062 @default.
• W2092201501 cites W2000568645 @default.
• W2092201501 cites W2000784221 @default.
• W2092201501 cites W2003675296 @default.
• W2092201501 cites W2003859096 @default.
• W2092201501 cites W2008617293 @default.
• W2092201501 cites W2009112986 @default.
• W2092201501 cites W2016661782 @default.
• W2092201501 cites W2017091317 @default.
• W2092201501 cites W2020310632 @default.
• W2092201501 cites W2020873558 @default.
• W2092201501 cites W2022733092 @default.
• W2092201501 cites W2023932333 @default.
• W2092201501 cites W2025653570 @default.
• W2092201501 cites W2026432913 @default.
• W2092201501 cites W2027732306 @default.
• W2092201501 cites W2027870283 @default.
• W2092201501 cites W2036117253 @default.
• W2092201501 cites W2036334370 @default.
• W2092201501 cites W2036350402 @default.
• W2092201501 cites W2037951645 @default.
• W2092201501 cites W2038950307 @default.
• W2092201501 cites W2038952987 @default.
• W2092201501 cites W2038997703 @default.
• W2092201501 cites W2043169858 @default.
• W2092201501 cites W2043861905 @default.
• W2092201501 cites W2045840200 @default.
• W2092201501 cites W2046047967 @default.
• W2092201501 cites W2046770192 @default.
• W2092201501 cites W2047319594 @default.
• W2092201501 cites W2047695493 @default.
• W2092201501 cites W2049742969 @default.
• W2092201501 cites W2054005605 @default.
• W2092201501 cites W2054506758 @default.
• W2092201501 cites W2054537362 @default.
• W2092201501 cites W2055280799 @default.
• W2092201501 cites W2057456250 @default.
• W2092201501 cites W2062162775 @default.
• W2092201501 cites W2064718789 @default.
• W2092201501 cites W2066176114 @default.
• W2092201501 cites W2067360027 @default.
• W2092201501 cites W2068089057 @default.
• W2092201501 cites W2068128836 @default.
• W2092201501 cites W2071485861 @default.
• W2092201501 cites W2072547324 @default.
• W2092201501 cites W2073626830 @default.
• W2092201501 cites W2074799494 @default.
• W2092201501 cites W2077904041 @default.
• W2092201501 cites W2078328888 @default.
• W2092201501 cites W2079955869 @default.
• W2092201501 cites W2081349327 @default.
• W2092201501 cites W2082371987 @default.
• W2092201501 cites W2083296465 @default.
• W2092201501 cites W2083805191 @default.
• W2092201501 cites W2086125606 @default.
• W2092201501 cites W2086341178 @default.
• W2092201501 cites W2090400305 @default.
• W2092201501 cites W2091080027 @default.
• W2092201501 cites W2091448229 @default.
• W2092201501 cites W2091871958 @default.
• W2092201501 cites W2093988328 @default.

• next