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• W2092201864 abstract "Objectives: Ovarian tissue cryopreservation is now becoming an important issue because of a steadily improvement on long-term survival rates for young people with malignant diseases with the additional need of preserving their fertility. The objectives of this study were: (1) to determine the feasibility of freezing and thawing ovarian tissue and (2) to compare the results of post-thaw effects with two different cryoprotectants: DMSO and propanediol. Design: Prospective and experimental study assessing the viability of ovarian tissue after freezing and thawing with DMSO or propanediol. It was performed in 15 sheep undergoing bilateral ooforectomy for further auto-transplantation. Materials and Methods: Bilateral oophorectomy were performed in 15 Kathadin sheep. The ovarian tissue was placed in α-minimum essential medium (α-MEM). The ovarian cortex was dissected using a scalpel to obtain pieces no thicker than 2 mm. The cortex was then divided into three pieces, one fixed in formaldehyde and sent to histologic evaluation and two frozen in either DMSO (1.5 M) or propanediol (1.5 M). Samples were gently rolled to promote rapid equilibration for 30 minutes at 4°C in 1ml cryogenic vials. Then, the samples were frozen under a slow freezing protocol started at −6°C at a rate of −0.5°C/min. Manual seeding were done al −7°C and a 5 minutes stabilization period was kept under the same temperature. When the temperature reached −40°C the vial were plunged into liquid nitrogen. Next day, a sample of each sheep with both cryoprotectants were thaw in air at room temperature for 2 minutes and then immersed in a water bath at 37°C until the ice had been thawed. The tissue pieces were then immediately transferred to α-MEM and washed three times before fixation with formaldehyde for histologic evaluation. Results: There were no differences between fresh and thawed ovarian tissue after histologic evaluation. There were no observed changes in the tissue components using a light microscope. Freezing and thawing caused no damage to the primordial and primary follicles. Even more, we were able to find several cavitary follicles. These findings were regardless of the cryoprotectant used. Mean number of primordial and primary follicles per high power field over 10 observations were the same in fresh (7.2 ± 3.2) when compared to post thaw specimens with DMSO (6.8 ± 4.1) and propanediol (6.3 ± 2.5). Conclusions: Ovarian tissue cryopreservation can be done without evidence of histologic damage, suggesting viability and possibility of auto-transplantation. DMSO and propanediol had similar results. Further conclusions will be obtained after these specimens are transplanted." @default.
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• W2092201864 title "Effect of Cryopreservation on Sheep Ovarian Tissue Comparing Two Cryoprotectants: Dimethylsulfoxide (DMSO) and 1,2-Propanediol" @default.
• W2092201864 doi "https://doi.org/10.1016/s0015-0282(00)01355-8" @default.
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