FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092244503> ?p ?o ?g. }

• W2092244503 endingPage "578" @default.
• W2092244503 startingPage "562" @default.
• W2092244503 abstract "Aerococcus viridansl-lactate oxidase (avLOX) is a biotechnologically important flavoenzyme that catalyzes the conversion of L-lactate and O₂ into pyruvate and H₂O₂. The enzymatic reaction underlies different biosensor applications of avLOX for blood L-lactate determination. The ability of avLOX to replace O₂ with other electron acceptors such as 2,6-dichlorophenol-indophenol (DCIP) allows the possiblity of analytical and practical applications. The A95G variant of avLOX was previously shown to exhibit lowered reactivity with O₂ compared to wild-type enzyme and therefore was employed in a detailed investigation with respect to the specificity for different electron acceptor substrates. From stopped-flow experiments performed at 20 °C (pH 6.5), we determined that the A95G variant (fully reduced by L-lactate) was approximately three-fold more reactive towards DCIP (1.0 ± 0.1 × 10(6) M(-1) ·s(-1) ) than O₂, whereas avLOX wild-type under the same conditions was 14-fold more reactive towards O₂(1.8 ± 0.1 × 10(6) m(-1) ·s(-1)) than DCIP. Substituted 1,4-benzoquinones were up to five-fold better electron acceptors for reaction with L-lactate-reduced A95G variant than wild-type. A 1.65-Å crystal structure of oxidized A95G variant bound with pyruvate was determined and revealed that the steric volume created by removal of the methyl side chain of Ala95 and a slight additional shift in the main chain at position Gly95 together enable the accomodation of a new active-site water molecule within hydrogen-bond distance to the N5 of the FMN cofactor. The increased steric volume available in the active site allows the A95G variant to exhibit a similar trend with the related glycolate oxidase in electron acceptor substrate specificities, despite the latter containing an alanine at the analogous position." @default.
• W2092244503 created "2016-06-24" @default.
• W2092244503 creator A5001355284 @default.
• W2092244503 creator A5023819817 @default.
• W2092244503 creator A5051203357 @default.
• W2092244503 creator A5059924773 @default.
• W2092244503 creator A5081056309 @default.
• W2092244503 date "2014-12-16" @default.
• W2092244503 modified "2023-10-05" @default.
• W2092244503 title "The Ala95-to-Gly substitution in<i>Aerococcus viridans</i><scp>l</scp>-lactate oxidase revisited - structural consequences at the catalytic site and effect on reactivity with O₂and other electron acceptors" @default.
• W2092244503 cites W1487272172 @default.
• W2092244503 cites W1502554469 @default.
• W2092244503 cites W1507208022 @default.
• W2092244503 cites W1593953517 @default.
• W2092244503 cites W1784475756 @default.
• W2092244503 cites W1969279038 @default.
• W2092244503 cites W1969536662 @default.
• W2092244503 cites W1977681633 @default.
• W2092244503 cites W1978096041 @default.
• W2092244503 cites W1978865842 @default.
• W2092244503 cites W1995118824 @default.
• W2092244503 cites W2000455675 @default.
• W2092244503 cites W2000608387 @default.
• W2092244503 cites W2001641653 @default.
• W2092244503 cites W2007359091 @default.
• W2092244503 cites W2008410684 @default.
• W2092244503 cites W2019119244 @default.
• W2092244503 cites W2026826346 @default.
• W2092244503 cites W2026836885 @default.
• W2092244503 cites W2035069526 @default.
• W2092244503 cites W2036570788 @default.
• W2092244503 cites W2038840577 @default.
• W2092244503 cites W2040505558 @default.
• W2092244503 cites W2043171318 @default.
• W2092244503 cites W2043203894 @default.
• W2092244503 cites W2043764853 @default.
• W2092244503 cites W2044416309 @default.
• W2092244503 cites W2046242399 @default.
• W2092244503 cites W2047131411 @default.
• W2092244503 cites W2048145086 @default.
• W2092244503 cites W2049785072 @default.
• W2092244503 cites W2050299609 @default.
• W2092244503 cites W2054943036 @default.
• W2092244503 cites W2055557373 @default.
• W2092244503 cites W2058657840 @default.
• W2092244503 cites W2058960367 @default.
• W2092244503 cites W2067020936 @default.
• W2092244503 cites W2072431472 @default.
• W2092244503 cites W2091870640 @default.
• W2092244503 cites W2101762173 @default.
• W2092244503 cites W2104473127 @default.
• W2092244503 cites W2110494948 @default.
• W2092244503 cites W2124026197 @default.
• W2092244503 cites W2160525763 @default.
• W2092244503 cites W2170520204 @default.
• W2092244503 cites W2317809844 @default.
• W2092244503 cites W2323563792 @default.
• W2092244503 cites W2325713750 @default.
• W2092244503 cites W2394678214 @default.
• W2092244503 cites W2405441062 @default.
• W2092244503 doi "https://doi.org/10.1111/febs.13162" @default.
• W2092244503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25423902" @default.
• W2092244503 hasPublicationYear "2014" @default.
• W2092244503 type Work @default.
• W2092244503 sameAs 2092244503 @default.
• W2092244503 citedByCount "12" @default.
• W2092244503 countsByYear W20922445032015 @default.
• W2092244503 countsByYear W20922445032016 @default.
• W2092244503 countsByYear W20922445032018 @default.
• W2092244503 countsByYear W20922445032020 @default.
• W2092244503 countsByYear W20922445032021 @default.
• W2092244503 countsByYear W20922445032022 @default.
• W2092244503 countsByYear W20922445032023 @default.
• W2092244503 crossrefType "journal-article" @default.
• W2092244503 hasAuthorship W2092244503A5001355284 @default.
• W2092244503 hasAuthorship W2092244503A5023819817 @default.
• W2092244503 hasAuthorship W2092244503A5051203357 @default.
• W2092244503 hasAuthorship W2092244503A5059924773 @default.
• W2092244503 hasAuthorship W2092244503A5081056309 @default.
• W2092244503 hasConcept C121332964 @default.
• W2092244503 hasConcept C142724271 @default.
• W2092244503 hasConcept C161790260 @default.
• W2092244503 hasConcept C185592680 @default.
• W2092244503 hasConcept C201194858 @default.
• W2092244503 hasConcept C204787440 @default.
• W2092244503 hasConcept C26873012 @default.
• W2092244503 hasConcept C2776910235 @default.
• W2092244503 hasConcept C2778417172 @default.
• W2092244503 hasConcept C2779892579 @default.
• W2092244503 hasConcept C2900893 @default.
• W2092244503 hasConcept C41183919 @default.
• W2092244503 hasConcept C55493867 @default.
• W2092244503 hasConcept C71240020 @default.
• W2092244503 hasConcept C71924100 @default.
• W2092244503 hasConcept C75473681 @default.
• W2092244503 hasConceptScore W2092244503C121332964 @default.
• W2092244503 hasConceptScore W2092244503C142724271 @default.
• W2092244503 hasConceptScore W2092244503C161790260 @default.

• next