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• W2092247380 abstract "Highly elevated partial pressures of oxygen achievable during hyperbaric oxygenation (HBO) have been shown to reduce leukocyte sequestration following ischemia/reperfusion injury suggesting a clinical role for HBO in treatment of various disease states characterized by transient ischemia. Previous studies have suggested that this effect may be due to inhibition of β2-integrin function. In this study the effect of HBO on various CD11b/CD18 (Mac-1) mediated neutrophil functions was investigated in healthy human subjects. HBO 3.0 ATA, 23 m reduced adhesion 50% at 2 h with return to pre-HBO levels by 6 h. Homotypic aggregation, a Mac-1 dependent function, under fluid shear following stimulation with f-MLP was reduced from 20±2.6 to 3.4±1.0% 2 h after HBO. However, HBO did not inhibit adhesion to IL-1β stimulated HUVEC. Mac-1 mediated oxidative burst induced by opsonized zymosan was reduced 38.2±10.6% (P<0.05) by HBO. However, oxidative burst induced by PMA or f-MLP was not affected. HBO did not alter the distribution of neutrophils displaying morphologies associated with stimulation (ruffled, bipolar, uropod) over a 24 h period after HBO nor did HBO change the percentages of mature versus immature cells. Taken together these findings demonstrate that HBO specifically inhibits Mac-1 mediated functions." @default.
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• W2092247380 date "2002-09-02" @default.
• W2092247380 modified "2023-09-27" @default.
• W2092247380 title "Hyperbaric oxygen exposure temporarily reduces Mac-1 mediated functions of human neutrophils" @default.
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• W2092247380 doi "https://doi.org/10.1016/s0165-2478(02)00068-8" @default.
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