Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092280503> ?p ?o ?g. }
- W2092280503 endingPage "748" @default.
- W2092280503 startingPage "741" @default.
- W2092280503 abstract "Hypsizygus marmoreus is one of the major edible mushrooms in East Asia. As no efficient transformation method, the molecular and genetics studies were hindered. The glyceraldehyde-3-phosphate dehydrogenase (GPD) gene of H. marmoreus was isolated and its promoter was used to drive the hygromycin B phosphotransferase (HPH) and enhanced green fluorescent protein (EGFP) in H. marmoreus . Agrobacterium tumefaciens -mediated transformation (ATMT) was successfully applied in H. marmoreus . The transformation parameters were optimized, and it was found that co-cultivation of bacteria with protoplast at a ratio of 1000:1 at a temperature of 26 °C in medium containing 0.3 mM acetosyringone resulted in the highest transformation efficiency for Agrobacterium strain. Besides, three plasmids, each carrying a different promoter (from H. marmoreus , Ganoderma lucidum and Lentinula edodes ) driving the expression of an antibiotic resistance marker, were also tested. The construct carrying the H. marmoreus gpd promoter produced more transformants than other constructs. Our analysis showed that over 85% of the transformants tested remained mitotically stable even after five successive rounds of subculturing. Putative transformants were analyzed for the presence of hph gene by PCR and Southern blot. Meanwhile, the expression of EGFP in H. marmoreus transformants was detected by fluorescence imaging. This ATMT system increases the transformation efficiency of H. marmoreus and may represent a useful tool for molecular genetic studies in this mushroom species." @default.
- W2092280503 created "2016-06-24" @default.
- W2092280503 creator A5011090841 @default.
- W2092280503 creator A5023856843 @default.
- W2092280503 creator A5033020983 @default.
- W2092280503 creator A5065417233 @default.
- W2092280503 creator A5067196763 @default.
- W2092280503 creator A5067728379 @default.
- W2092280503 creator A5074788667 @default.
- W2092280503 creator A5082306224 @default.
- W2092280503 creator A5089205591 @default.
- W2092280503 date "2014-09-01" @default.
- W2092280503 modified "2023-09-24" @default.
- W2092280503 title "An efficient Agrobacterium-mediated transformation method for the edible mushroom Hypsizygus marmoreus" @default.
- W2092280503 cites W1963923999 @default.
- W2092280503 cites W1968422424 @default.
- W2092280503 cites W1981707478 @default.
- W2092280503 cites W1995756803 @default.
- W2092280503 cites W2000800268 @default.
- W2092280503 cites W2006892257 @default.
- W2092280503 cites W2009531811 @default.
- W2092280503 cites W2011760613 @default.
- W2092280503 cites W2014235002 @default.
- W2092280503 cites W2020689048 @default.
- W2092280503 cites W2023526422 @default.
- W2092280503 cites W2027741551 @default.
- W2092280503 cites W2028275573 @default.
- W2092280503 cites W2029633127 @default.
- W2092280503 cites W2035089399 @default.
- W2092280503 cites W2036245156 @default.
- W2092280503 cites W2042551683 @default.
- W2092280503 cites W2042653627 @default.
- W2092280503 cites W2043168235 @default.
- W2092280503 cites W2052086287 @default.
- W2092280503 cites W2054051667 @default.
- W2092280503 cites W2058482032 @default.
- W2092280503 cites W2067206496 @default.
- W2092280503 cites W2069575416 @default.
- W2092280503 cites W2079007471 @default.
- W2092280503 cites W2091308083 @default.
- W2092280503 cites W2092612150 @default.
- W2092280503 cites W2093008319 @default.
- W2092280503 cites W2096879929 @default.
- W2092280503 cites W2102713581 @default.
- W2092280503 cites W2107365509 @default.
- W2092280503 cites W2108411329 @default.
- W2092280503 cites W2135344138 @default.
- W2092280503 cites W2140289048 @default.
- W2092280503 cites W2164261080 @default.
- W2092280503 doi "https://doi.org/10.1016/j.micres.2014.01.004" @default.
- W2092280503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24612605" @default.
- W2092280503 hasPublicationYear "2014" @default.
- W2092280503 type Work @default.
- W2092280503 sameAs 2092280503 @default.
- W2092280503 citedByCount "41" @default.
- W2092280503 countsByYear W20922805032015 @default.
- W2092280503 countsByYear W20922805032016 @default.
- W2092280503 countsByYear W20922805032017 @default.
- W2092280503 countsByYear W20922805032018 @default.
- W2092280503 countsByYear W20922805032019 @default.
- W2092280503 countsByYear W20922805032020 @default.
- W2092280503 countsByYear W20922805032021 @default.
- W2092280503 countsByYear W20922805032022 @default.
- W2092280503 countsByYear W20922805032023 @default.
- W2092280503 crossrefType "journal-article" @default.
- W2092280503 hasAuthorship W2092280503A5011090841 @default.
- W2092280503 hasAuthorship W2092280503A5023856843 @default.
- W2092280503 hasAuthorship W2092280503A5033020983 @default.
- W2092280503 hasAuthorship W2092280503A5065417233 @default.
- W2092280503 hasAuthorship W2092280503A5067196763 @default.
- W2092280503 hasAuthorship W2092280503A5067728379 @default.
- W2092280503 hasAuthorship W2092280503A5074788667 @default.
- W2092280503 hasAuthorship W2092280503A5082306224 @default.
- W2092280503 hasAuthorship W2092280503A5089205591 @default.
- W2092280503 hasConcept C104317684 @default.
- W2092280503 hasConcept C153911025 @default.
- W2092280503 hasConcept C173749725 @default.
- W2092280503 hasConcept C178050479 @default.
- W2092280503 hasConcept C204241405 @default.
- W2092280503 hasConcept C22744801 @default.
- W2092280503 hasConcept C2776162087 @default.
- W2092280503 hasConcept C2776236347 @default.
- W2092280503 hasConcept C2776474821 @default.
- W2092280503 hasConcept C2779656422 @default.
- W2092280503 hasConcept C2780058085 @default.
- W2092280503 hasConcept C2780208070 @default.
- W2092280503 hasConcept C2780874738 @default.
- W2092280503 hasConcept C33161422 @default.
- W2092280503 hasConcept C40767141 @default.
- W2092280503 hasConcept C54355233 @default.
- W2092280503 hasConcept C59822182 @default.
- W2092280503 hasConcept C86803240 @default.
- W2092280503 hasConcept C89423630 @default.
- W2092280503 hasConceptScore W2092280503C104317684 @default.
- W2092280503 hasConceptScore W2092280503C153911025 @default.
- W2092280503 hasConceptScore W2092280503C173749725 @default.
- W2092280503 hasConceptScore W2092280503C178050479 @default.
- W2092280503 hasConceptScore W2092280503C204241405 @default.