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- W2092302358 abstract "We have developed an improved assay for the production of collagen by fibroblasts. Early passage adult mouse lung fibroblasts, established and maintained in serum-free culture, were employed as the target cells. An enzyme immunoassay was used for detection of type I collagen deposited on the substratum, permitting adaptation of the technique to cultures in 96-well microplates. Approximately two-fold enhancement of collagen deposition was induced by exposure to a concentration of 3 ng/ml of transforming growth factor-β1 or of 100 ng/ml of insulin-like growth factor-1 for 48 hr." @default.
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- W2092302358 date "1994-01-01" @default.
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- W2092302358 title "Immunoenzymatic Detection of Collagen Production by Pulmonary Fibroblasts in Serum-Free Culture" @default.
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- W2092302358 doi "https://doi.org/10.3109/10520299409106303" @default.
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