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- W2092309240 abstract "The use of microsatellite markers in large-scale genetic studies is limited by its low throughput and high cost and labor requirements. Here, we provide a panel of 45 multiplex PCRs for fast and cost-efficient genome-wide fluorescence-based microsatellite analysis in grapevine. The developed multiplex PCRs panel (with up to 15-plex) enables the scoring of 270 loci covering all the grapevine genome (9 to 20 loci/chromosome) using only 45 PCRs and sequencer runs. The 45 multiplex PCRs were validated using a diverse grapevine collection of 207 accessions, selected to represent most of the cultivated Vitis vinifera genetic diversity. Particular attention was paid to quality control throughout the whole process (assay replication, null allele detection, ease of scoring). Genetic diversity summary statistics and features of electrophoretic profiles for each studied marker are provided, as are the genotypes of 25 common cultivars that could be used as references in other studies." @default.
- W2092309240 created "2016-06-24" @default.
- W2092309240 creator A5008867644 @default.
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- W2092309240 date "2015-02-05" @default.
- W2092309240 modified "2023-09-25" @default.
- W2092309240 title "Whole-genome genotyping of grape using a panel of microsatellite multiplex PCRs" @default.
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- W2092309240 doi "https://doi.org/10.1007/s11295-015-0843-4" @default.
- W2092309240 hasPublicationYear "2015" @default.
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