Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092320171> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2092320171 endingPage "304" @default.
- W2092320171 startingPage "293" @default.
- W2092320171 abstract "Subtilisin BPN' is an extracellular serine protease from Bacillus amyloliquefaciens that requires an N-terminal 77 amino acid pro-sequence for correct folding of the catalytic domain. We have expressed an inactive, stable pro-subtilisin variant in Escherichia coli and show that it has structural properties similar to native subtilisin in terms of its near- and far-UV circular dichroism spectra, its compactness, and its capacity to bind calcium ions stoichiometrically. Unlike subtilisin, the pro-subtilisin variant unfolds reversibly with guanidinium chloride, and unfolding occurs via a folding intermediate. This intermediate is similar to the metastable intermediate state recently found for folding of subtilisin in the absence of the pro-sequence. The intermediate state has native-like secondary but little tertiary structure, and has a compactness between that of the native and unfolded state. Pro-subtilisin folds from the intermediate to the folded state in a single co-operative transition mediated by the pro-sequence. The isolated pro-sequence does not appear from its circular dichroism and 1H-NMR spectrum to have enough intrinsic stabilizing interactions to fold autonomously. However, the difference circular dichroism spectra of the pro-subtilisin variant and native subtilisin suggest that it is folded in the context of the pro-subtilisin molecule. The inability of the pro-subtilisin variant to bind a polypeptide inhibitor supports further the hypothesis that the pro-sequence interacts with subtilisin in the region where the active site is exposed. Our results suggest that the interactions provided by the pro-sequence are important only late on the folding pathway of pro-subtilisin and stabilize the transition state for folding. Kinetic analysis of the refolding reaction in the presence and absence of the pro-sequence reveal this stabilization to be in excess of 7.5 kcal/mol; folding is accelerated more than five orders of magnitude." @default.
- W2092320171 created "2016-06-24" @default.
- W2092320171 creator A5030415981 @default.
- W2092320171 creator A5068711489 @default.
- W2092320171 creator A5085433812 @default.
- W2092320171 date "1993-09-01" @default.
- W2092320171 modified "2023-10-02" @default.
- W2092320171 title "Folding of Subtilisin BPN': Role of the Pro-sequence" @default.
- W2092320171 doi "https://doi.org/10.1006/jmbi.1993.1507" @default.
- W2092320171 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8377204" @default.
- W2092320171 hasPublicationYear "1993" @default.
- W2092320171 type Work @default.
- W2092320171 sameAs 2092320171 @default.
- W2092320171 citedByCount "107" @default.
- W2092320171 countsByYear W20923201712012 @default.
- W2092320171 countsByYear W20923201712013 @default.
- W2092320171 countsByYear W20923201712014 @default.
- W2092320171 countsByYear W20923201712015 @default.
- W2092320171 countsByYear W20923201712016 @default.
- W2092320171 countsByYear W20923201712017 @default.
- W2092320171 countsByYear W20923201712022 @default.
- W2092320171 countsByYear W20923201712023 @default.
- W2092320171 crossrefType "journal-article" @default.
- W2092320171 hasAuthorship W2092320171A5030415981 @default.
- W2092320171 hasAuthorship W2092320171A5068711489 @default.
- W2092320171 hasAuthorship W2092320171A5085433812 @default.
- W2092320171 hasConcept C104317684 @default.
- W2092320171 hasConcept C119599485 @default.
- W2092320171 hasConcept C127413603 @default.
- W2092320171 hasConcept C133571119 @default.
- W2092320171 hasConcept C167625842 @default.
- W2092320171 hasConcept C181199279 @default.
- W2092320171 hasConcept C185592680 @default.
- W2092320171 hasConcept C204328495 @default.
- W2092320171 hasConcept C2776414213 @default.
- W2092320171 hasConcept C2776545253 @default.
- W2092320171 hasConcept C2776714187 @default.
- W2092320171 hasConcept C2777807008 @default.
- W2092320171 hasConcept C2779811187 @default.
- W2092320171 hasConcept C55493867 @default.
- W2092320171 hasConcept C71240020 @default.
- W2092320171 hasConcept C75599170 @default.
- W2092320171 hasConcept C8010536 @default.
- W2092320171 hasConceptScore W2092320171C104317684 @default.
- W2092320171 hasConceptScore W2092320171C119599485 @default.
- W2092320171 hasConceptScore W2092320171C127413603 @default.
- W2092320171 hasConceptScore W2092320171C133571119 @default.
- W2092320171 hasConceptScore W2092320171C167625842 @default.
- W2092320171 hasConceptScore W2092320171C181199279 @default.
- W2092320171 hasConceptScore W2092320171C185592680 @default.
- W2092320171 hasConceptScore W2092320171C204328495 @default.
- W2092320171 hasConceptScore W2092320171C2776414213 @default.
- W2092320171 hasConceptScore W2092320171C2776545253 @default.
- W2092320171 hasConceptScore W2092320171C2776714187 @default.
- W2092320171 hasConceptScore W2092320171C2777807008 @default.
- W2092320171 hasConceptScore W2092320171C2779811187 @default.
- W2092320171 hasConceptScore W2092320171C55493867 @default.
- W2092320171 hasConceptScore W2092320171C71240020 @default.
- W2092320171 hasConceptScore W2092320171C75599170 @default.
- W2092320171 hasConceptScore W2092320171C8010536 @default.
- W2092320171 hasIssue "2" @default.
- W2092320171 hasLocation W20923201711 @default.
- W2092320171 hasLocation W20923201712 @default.
- W2092320171 hasOpenAccess W2092320171 @default.
- W2092320171 hasPrimaryLocation W20923201711 @default.
- W2092320171 hasRelatedWork W149869174 @default.
- W2092320171 hasRelatedWork W1976007673 @default.
- W2092320171 hasRelatedWork W1982656769 @default.
- W2092320171 hasRelatedWork W2002182575 @default.
- W2092320171 hasRelatedWork W2065384285 @default.
- W2092320171 hasRelatedWork W2085694096 @default.
- W2092320171 hasRelatedWork W2092320171 @default.
- W2092320171 hasRelatedWork W4207042532 @default.
- W2092320171 hasRelatedWork W996828143 @default.
- W2092320171 hasRelatedWork W2106807130 @default.
- W2092320171 hasVolume "233" @default.
- W2092320171 isParatext "false" @default.
- W2092320171 isRetracted "false" @default.
- W2092320171 magId "2092320171" @default.
- W2092320171 workType "article" @default.