Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092332610> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2092332610 endingPage "210" @default.
- W2092332610 startingPage "204" @default.
- W2092332610 abstract "Thermostable pullulanase was purified to homogeneity on sodium dodecyl sulfate-polyacrylamide gel from the culture supernatant of Bacillus stearothermophilus TRS128. However, multiformity of the pullulanase was suggested by activity staining on a pullulan-reactive red plate. The thermostability of the enzyme was tested. In the presence of Ca2+, the optimum temperature of the pullulanase was 75°C, and nearly 100% of the enzyme activity was retained even after treatment at 68°C for 60 min. Since the thermostable pullulanase gene (pulT) has been cloned, the nucleotide sequence was determined. Although the DNA sequence revealed only one large open reading frame, two possible pairs of SD sequence and initiation codon were found in the frame. To analyze the regulatory region, several mutations (deletion, insertion and substitution of nucleotides) were introduced in the flanking region of pulT, using site-directed mutagenesis. A putative promoter, SD sequence and initiation codon were inferred. The pulT gene was composed of 1974 bases and 658 amino acid residues (molecular weight 75,375). The deduced amino acid sequence of the thermostable pullulanase exhibited a fairly low homology with that of the thermolabile pullulanase from Klebsiella aerogenes. However, four consensus sequences containing catalytic and/or substrate binding sites for amylolytic enzymes were also found in the thermostable pullulanase and the thermolabile enzyme." @default.
- W2092332610 created "2016-06-24" @default.
- W2092332610 creator A5001759427 @default.
- W2092332610 creator A5002050470 @default.
- W2092332610 creator A5015755327 @default.
- W2092332610 date "1990-01-01" @default.
- W2092332610 modified "2023-10-15" @default.
- W2092332610 title "Characteristics of thermostable pullulanase from Bacillus stearothermophilus and the nucleotide sequence of the gene" @default.
- W2092332610 cites W1482797004 @default.
- W2092332610 cites W1505429636 @default.
- W2092332610 cites W1517079798 @default.
- W2092332610 cites W1530477060 @default.
- W2092332610 cites W1551920028 @default.
- W2092332610 cites W1555021163 @default.
- W2092332610 cites W1678106742 @default.
- W2092332610 cites W1697346670 @default.
- W2092332610 cites W173588649 @default.
- W2092332610 cites W1838414788 @default.
- W2092332610 cites W1839791681 @default.
- W2092332610 cites W1874425932 @default.
- W2092332610 cites W1910070568 @default.
- W2092332610 cites W1918349277 @default.
- W2092332610 cites W1981777358 @default.
- W2092332610 cites W1983162858 @default.
- W2092332610 cites W1986002586 @default.
- W2092332610 cites W2012272263 @default.
- W2092332610 cites W2028954926 @default.
- W2092332610 cites W2052774650 @default.
- W2092332610 cites W2100837269 @default.
- W2092332610 cites W2104176325 @default.
- W2092332610 cites W2114624600 @default.
- W2092332610 cites W2120024227 @default.
- W2092332610 cites W2340576404 @default.
- W2092332610 cites W4254839445 @default.
- W2092332610 doi "https://doi.org/10.1016/0922-338x(90)90213-g" @default.
- W2092332610 hasPublicationYear "1990" @default.
- W2092332610 type Work @default.
- W2092332610 sameAs 2092332610 @default.
- W2092332610 citedByCount "46" @default.
- W2092332610 countsByYear W20923326102013 @default.
- W2092332610 countsByYear W20923326102014 @default.
- W2092332610 countsByYear W20923326102015 @default.
- W2092332610 countsByYear W20923326102016 @default.
- W2092332610 countsByYear W20923326102018 @default.
- W2092332610 countsByYear W20923326102019 @default.
- W2092332610 countsByYear W20923326102020 @default.
- W2092332610 countsByYear W20923326102022 @default.
- W2092332610 crossrefType "journal-article" @default.
- W2092332610 hasAuthorship W2092332610A5001759427 @default.
- W2092332610 hasAuthorship W2092332610A5002050470 @default.
- W2092332610 hasAuthorship W2092332610A5015755327 @default.
- W2092332610 hasConcept C104317684 @default.
- W2092332610 hasConcept C153911025 @default.
- W2092332610 hasConcept C167625842 @default.
- W2092332610 hasConcept C181199279 @default.
- W2092332610 hasConcept C2779155592 @default.
- W2092332610 hasConcept C2779423947 @default.
- W2092332610 hasConcept C55493867 @default.
- W2092332610 hasConcept C84148353 @default.
- W2092332610 hasConcept C86803240 @default.
- W2092332610 hasConceptScore W2092332610C104317684 @default.
- W2092332610 hasConceptScore W2092332610C153911025 @default.
- W2092332610 hasConceptScore W2092332610C167625842 @default.
- W2092332610 hasConceptScore W2092332610C181199279 @default.
- W2092332610 hasConceptScore W2092332610C2779155592 @default.
- W2092332610 hasConceptScore W2092332610C2779423947 @default.
- W2092332610 hasConceptScore W2092332610C55493867 @default.
- W2092332610 hasConceptScore W2092332610C84148353 @default.
- W2092332610 hasConceptScore W2092332610C86803240 @default.
- W2092332610 hasIssue "4" @default.
- W2092332610 hasLocation W20923326101 @default.
- W2092332610 hasOpenAccess W2092332610 @default.
- W2092332610 hasPrimaryLocation W20923326101 @default.
- W2092332610 hasRelatedWork W1767767984 @default.
- W2092332610 hasRelatedWork W1894156927 @default.
- W2092332610 hasRelatedWork W1921711018 @default.
- W2092332610 hasRelatedWork W1983195014 @default.
- W2092332610 hasRelatedWork W2004320955 @default.
- W2092332610 hasRelatedWork W2024063904 @default.
- W2092332610 hasRelatedWork W2073773093 @default.
- W2092332610 hasRelatedWork W2102895353 @default.
- W2092332610 hasRelatedWork W2118921831 @default.
- W2092332610 hasRelatedWork W2401542020 @default.
- W2092332610 hasVolume "69" @default.
- W2092332610 isParatext "false" @default.
- W2092332610 isRetracted "false" @default.
- W2092332610 magId "2092332610" @default.
- W2092332610 workType "article" @default.