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- W2092352563 abstract "Among new fluoroquinolone derivatives, the N-cyclopropylanalogs Ciprofloxacin (CIPRO) and ofloxacin (OFLO) have recently been found to have a high in vitro activity against a variety of mycobacteria (2). In this study, we have investigated their action against intracellularly growing Mycobacterium tuberculosis. For this purpose, the J-774 macrophage cell line was infected with the H37Rv type strain of M. tuberculosis, and drugs (at their serum level concentrations obtainable in healthy individuals (= 5 [µg/ml; 8) were added after 2 days of intracellular growth of the bacteria. The bacterial growth during the experimentation was followed both electron microscopically and also by lysing the macrophages at various time intervals, and enumerating the bacterial viable counts after plating the lysate on appropriate media. Both drugs at the concentrations used did not affect macrophage viability (as assessed by trypan blue staining), and were highly bactericidal against the virulent tubercle bacilli multiplying in our J-774 macrophage model. Kürzlich wurde festgestellt, daß aus der Reihe der neuen Fluorchinolonderivate die N-cyclopropylanalogen Ciprofloxacin (CIPRO) and Ofloxacin (OFLO) eine hohe in vitro-Aktivität gegen verschiedene Mycobakterien aufweisen (2). In der vorliegenden Arbeit haben wir ihre Wirkung gegen intrazellulär wachsende Mycobacterium tuberculosis untersucht. Zu diesem Zweck wurde die Makrophagen-Zellinie J-774 mit dem Typstamm H37Rv von M. tuberculosis infiziert. Nach 2 Tagen intrazellulären Wachstums der Bakterien wurden die Arzneimittel in einer der Serumspiegeln bei Gesunden entsprechenden Konzentration von 5 µg/ml (8) zugegeben. Das Bakterienwachstum während des Versuchs wurde sowohl elektronenmikroskopisch als auch durch Lysis der Makrophagen in verschiedenen Zeitabständen and Keimzahlbestimmung nach Ausstrich des Lysats auf geeignete Nährboden verfolgt. Bei den verwendeten Konzentrationen beeinflußten beide Arzneimittel die Lebensfähigkeit der Makrophagen nicht (was durch Trypanblau-Anfärbung ermittelt wurde) and zeigten eine hohe bakterizide Wirkung gegen die sich in unserem J-774-Makrophagen-Modell vermehrenden virulenten Tuberkelbazillen." @default.
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- W2092352563 date "1990-06-01" @default.
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- W2092352563 title "Intracellular Bactericidal Activity of Ciprofloxacin and Ofloxacin Against Mycobacterium tuberculosis H37Rv Multiplying in the J-774 Macrophage Cell Line" @default.
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- W2092352563 doi "https://doi.org/10.1016/s0934-8840(11)80249-5" @default.
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